Ecofriendly nanomaterials for controlling gray mold of table grapes and maintaining postharvest quality
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Biodegradable antifungal nanomaterials are a recent novel measure against plant pathogens. In the present investigation, the synthesis and characterization of some ecofriendly nanomaterials, including silica, chitosan, and copper nanoparticles (NPs) and their combination, were carried out. Their fungicidal activity was studied in vitro and in vivo against Botrytis cinerea, the causal agent of gray mold on table grapes. In addition, the effect of those nanomaterials on physical and chemical properties of grape (TSS, TA, TSS/TA ratio and berries colour) were evaluated. Scanning electron microscopy (SEM) and analysis of DNA-binding profile were used to better understand their mechanism of action. SEM showed that chitosan and silica NPs caused inhibition of hyphal growth and/or alteration of hyphal morphology such as cell wall disruption, withering, and excessive septation. NPs interacted with DNA isolated from fungal mats: the highest concentration of chitosan and silica NPs affected DNA integrity and led to a significant degradation. A single application of chitosan or silica NPs at veraison stage was able to reduce gray mold of table grapes. Although further large scale trials are needed, the promising results of this research suggest nanomaterials compounds, i.e. silica and chitosan NPs, as effective antifungal agents for the control of gray mold of table grapes.
KeywordsNanomaterials Gray mold Grape quality Scanning electron microscope Postharvest
This research was supported by the International Foundation for Science, Stockholm, Sweden, through a grant to Ms. Ayat F. Hashim (F5853).
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest. The manuscript was prepared under compliance with ethical standards.
Animal studies and human participants
This article does not contain any studies with human participants or animal performed by any of the authors.
- Abd-Elsalam, K. A., Vasil’kov, Y. A., Said-Galiev, E. E., Rubina, M. S., Khokhlov, A. R., Naumkin, A. V., Shtykova, E. V., & Alghuthaymi, M. A. (2018). Bimetallic blends and chitosan nanocomposites: Novel antifungal agents against cotton seedling damping-off. European Journal of Plant Pathology, 151, 57–72.Google Scholar
- Abourida, M., & Harb, F. (2014). Synthesis and characterization of amorphous silica Nanoparitcles from aqueous silicates using cationic surfactants. Journal of Metals, Materials and Minerals, 24, 37–42.Google Scholar
- Ahmed, A. I. S. (2017). Chitosan and silver nanoparticles as control agents of some Faba bean spot diseases. Journal of Plant Pathology and Microbiology, 8, 421.Google Scholar
- Barnett, H. L., & Hunter, B. B. (1986). Illustrated genera of imperfect Fungi (4th ed.). New York: Macmillan Publishing Co. 218 pp.Google Scholar
- Bernardos, A., Marina, T., Žáˇcek, P., Pérez-Esteve, É., Martínez-Máñez, R., Lhotka, M., Kouřimská, L., Pulkrábek, J., & Klouček, P. (2015). Antifungal effect of essential oil components against Aspergillus niger when loaded into silica mesoporous supports. Journal of the Science of Food and Agriculture, 95, 2824–2831.CrossRefGoogle Scholar
- Bowen, P., Menzies, J., Ehret, D., Samuels, L., & Glass, A. D. M. (1992). Soluble silicon sprays inhibit powdery mildew development on grape leaves. Journal of the American Society for Horticultural Science, 117, 906–912.Google Scholar
- Dang, T. M. D., Le, T. T. T., Fribourg-Blanc, E., & Dang, M. C. (2011). Synthesis and optical properties of copper nanoparticles prepared by a chemical reduction method. Advances in Natural Sciences: Nanoscience and Nanotechnology, 2, 015009.Google Scholar
- Dann, E. K., & Muir, S. (2002). Peas grown in media with elevated plant-available silicon levels have higher activities of chitinase and β-1,3-glucanase, are less susceptible to a fungal leaf spot pathogen and accumulate more foliar silicon. Australasian Plant Pathology, 31, 9–13.CrossRefGoogle Scholar
- Hernández-Lauzardo, A., Velázquez, M., & Guerra-Sánchez, M. (2011). Current status of action mode and effect of chitosan against phytopathogens fungi. African Journal of Microbiology Research, 5, 4243–4247.Google Scholar
- Joselito, D., & Soytong, K. (2014). Construction and characterization of copolymer nanomaterials loaded with bioactive compounds from Chaetomium species. Journal of Agricultural Technology, 10(4), 823–831.Google Scholar
- Menzies, J., Bowen, P., Ehret, D., & Glass, A. D. M. (1992). Foliar application of potassium silicate reduce severity of powdery mildew on cucumber, muskmelon, and zucchini squash. Journal of the American Society for Horticultural Science, 117, 902–905.Google Scholar
- Mohammadi, A., Hashemi, M., & Hosseini, S. M. (2015). Nanoencapsulation of Zataria multiflora essential oil preparation and characterization with enhanced antifungal growth for controlling Botrytis cinerea, the causal agent of gray mould disease. Innovative Food Science & Emerging Technologies, 14, 78–84.Google Scholar
- Nair, R. & Kumar, D.S. (2013). Plant Diseases—Control and Remedy Through Nanotechnology. pp. 231–244. Book Crop Improvement Under Adverse Conditions Edited by Narendra Tuteja and Sarvajeet Singh Gill.Google Scholar
- Saharan, V., Sharma, G., Yadav, M., Choudhary, M. K., Sharma, S. S., Pal, A., Raliya, R., & Biswas, P. (2015). Synthesis and in vitro antifungal efficacy of cu–chitosan nanoparticles against pathogenic fungi of tomato. International Journal of Biological Macromolecules, 75, 346–353.CrossRefGoogle Scholar
- Sanford, P. A. (2003). Commercial sources of chitin and chitosan and their utilization. In K. M. Varum, A. Domard, & O. SmidsrØd (Eds.), Advances in chitin science (Vol. 6, pp. 35–42). Trondheim: NTNU.Google Scholar
- Suhartono, D. (2015). Preparation of chitosan material and its antifungal activity for bamboo. International Journal of Science and Research, 6, 1586–1590.Google Scholar
- Usman, M. S., El Zowalaty, M. E., Shameli, K., Zainuddin, N., Salama, M., & Ibrahim, N. A. (2013). Synthesis, characterization, and antimicrobial properties of copper nanoparticles. International Journal of Nanomedicine, 18, 4467–4479.Google Scholar
- Youssef, K., Hashim, A. F., Margarita, R., Alghuthaymi, M. A., & Abd-Elsalam, K. A. (2017). Antifungal efficacy of chemically-produced copper nanoparticles against Penicillium digitatum and Fusarium solani on Citrus fruit. The Philippine Agricultural Scientist, 100(1), 69–78.Google Scholar