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Developing a methodology for identifying brown rot resistance in stone fruit

  • Núria Baró-Montel
  • Rosario Torres
  • Carla Casals
  • Neus Teixidó
  • Joan Segarra
  • Josep UsallEmail author
Article

Abstract

Assays were conducted to understand the effect of various factors involved with screening for resistance to Monilinia fructicola in stone fruit. First, the effect of maturity heterogeneity was determined in a set of fruit graded into three apparent maturity classes according to the IAD index for measurements taken with a DA-Meter. Second, different conidia concentrations, incubation times and M. fructicola strains were evaluated to optimize the methodology. Furthermore, the effects of fruit disinfection on M. fructicola, Penicillium expansum and Rhizopus stolonifer growth were studied. Finally, the developed methodology was applied to a set of commercial varieties of stone fruit to determine its suitability for identifying the level of susceptibility to brown rot. The results obtained indicated significant differences between wounded and non-wounded fruit, inoculum concentration and incubation time. The effect of strain aggressiveness was also confirmed, whereas, in general, no significant differences were observed among the fruit collected at the same harvest date. Our results also show that disinfection of fruit prior to inoculation had an effect on the infection process. In addition, the use of this methodology in commercial varieties of stone fruit allowed distinctions to be made among the levels of susceptibility to brown rot. Hence, the developed methodology could be applied to resistance screening in breeding programmes, as well as studying the genetic basis of brown rot resistance.

Keywords

Monilinia fructicola Prunus persica Disease resistance Peach Nectarine 

Notes

Acknowledgements

Authors are grateful to the Spanish Government for their financial support by national projects AGL2014-55287-C02-02-R and AGL2017-84389-C2-1-R from Ministry of Economy, Industry and Competitiveness (MINECO), to the Catalan Government (Generalitat de Catalunya) for the PhD grant 2016FI_B 00442 (Baró-Montel, N.) and for the funding received from CERCA Programme / Generalitat de Catalunya.

Compliance with ethical standards

Conflicts of interest

The authors declare that they have no conflicts of interest.

Research involving human participants and/or animals

This study does not include any trial with human and/or animals.

Informed consent

Informed consent was obtained from all of the individual participants included in the study.

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Copyright information

© Koninklijke Nederlandse Planteziektenkundige Vereniging 2018

Authors and Affiliations

  1. 1.IRTA, XaRTA-Postharvest, Edifici FruitcentreParc Científic i Tecnològic Agroalimentari de LleidaLleidaSpain
  2. 2.Department of Crop and Forest SciencesUniversity of LleidaLleidaSpain

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