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Identification and characterization of complete genome sequence of Alfalfa mosaic virus infecting Gynostemma pentaphyllum

  • Shuang Song
  • Huan Liu
  • Junhua Zhang
  • Chunqing Pan
  • Zhengnan Li
Article
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Abstract

Gynostemma pentaphyllum is a widely cultivated Chinese medicinal herb which has broad-spectrum activities. In this study, G. pentaphyllum plants with typical symptom of mosaic were found in Shaanxi province of China. The infection of Alfalfa mosaic virus (AMV) was identified by small RNA sequencing and confirmed by specific RT-PCR. We determined the complete genomic sequence of AMV-Gyn (GenBank No. MH332897-MH332899). The 3643 nt RNA1 and 2598 nt RNA2 encoded P1 protein of 1126 aa and P2 protein of 791 aa, respectively. The 2040 nt RNA3 encoded MP of 300 aa and CP of 221 aa. Phylogenetic analysis based on sequences of RNA3 and CP gene clustered AMV-Gyn into phylogroup I with the closest evolutionary relationship with AMV-Manfredi (KC881010). No recombination event was detected. To our knowledge, this is the first report of a virus infecting G. pentaphyllum.

Keywords

AMV Gynostemma pentaphyllum Small RNA sequencing Genome 

Notes

Funding

This study was funded by the “Young Talents” Project of Northeast Agricultural University (No. 17QC05) and University Nursing Program for Young Scholars with Creative Talents in Heilongjiang Province (No. uNPYSCT-2018157).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.

Informed consent

Informed consent was obtained from all individual participants included in the study.

Supplementary material

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Copyright information

© Koninklijke Nederlandse Planteziektenkundige Vereniging 2018

Authors and Affiliations

  • Shuang Song
    • 1
  • Huan Liu
    • 3
  • Junhua Zhang
    • 1
  • Chunqing Pan
    • 1
  • Zhengnan Li
    • 2
  1. 1.Department of Plant Protection, College of AgricultureNortheast Agricultural UniversityHarbinChina
  2. 2.College of Horticulture and Plant ProtectionInner Mongolia Agricultural UniversityHuhhotChina
  3. 3.State Key Laboratory of Crop Stress Biology for Arid Areas, College of Plant ProtectionNorthwest A&F UniversityYanglingChina

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