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European Journal of Plant Pathology

, Volume 153, Issue 3, pp 947–955 | Cite as

Diversity and geographic distribution of fungal strains infecting field-grown common bean (Phaseolus vulgaris L.) in Tunisia

  • Yosra Sendi
  • Samir Ben Romdhane
  • Ridha Mhamdi
  • Moncef MrabetEmail author
Article
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Abstract

A collection of 103 fungal strains was established from infected common bean plants (Phaseolus vulgaris L.) field-grown in three geographic regions from Tunisia and known for their long history in bean culture; Boucharray, Chatt-Mariem, and Metline. The fungal strain collection was established from common bean root and aerial parts. The pathogenicity test carried out on germinated seedlings showed that among the fungal collection, 41% of fungal strains were assigned to be highly pathogenic. In fact, serious cases of seedling damping-off, as well as a significant reduction in root and shoot biomass in cv. Coco blanc were noticed (up to 90% biomass reduction) considering fungal strains from the three prospected localities. The identification of fungal isolates belonging to this high pathogenicity class, based on the internal transcribed spacer region (ITS), showed a wide generic and specific diversity among common bean pathogenic fungi in Tunisia. Fusarium spp. strains were dominant and represented 67% of the characterized fungal collection. Fungal genera including Alternaria (22%), Rhizoctonia (4%), Ascomycota (4%), Macrophomina (10%) and Phoma (4%) were also reported. The highest richness levels were found in the Chatt-Mariem and Boucharray regions, showing the highest generic and interspecific diversity. In this work, we revealed also a variability in the abundance and geographic distribution of fungal species between the three prospected regions. Fungal strains infecting common bean in Metline were represented exclusively by Fusarium oxysporum. However, the genus Fusarium represented about 66% of fungal strains recovered from Boucharray, and only 20% from Chatt-Mariem. The genus Alternaria represented 11% and 40% of total fungal isolates in Boucharray and Chatt-Mariem, respectively, and was isolated only from the foliar parts of diseased common bean plants. The present work represents an important database that should be considered for surveying common bean fungal diseases.

Keywords

Common bean Fungi Pathogenicity Internal transcribed spacer Diversity 

Notes

Acknowledgements

This work was supported by the Ministry of Higher Education and Scientific Research of Tunisia under Grant 2015-2018 “Improvement of Legume Production”for the Laboratory of Legumes, Centre of Biotechnology of Borj-Cédria.

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Copyright information

© Koninklijke Nederlandse Planteziektenkundige Vereniging 2018

Authors and Affiliations

  • Yosra Sendi
    • 1
    • 2
  • Samir Ben Romdhane
    • 2
  • Ridha Mhamdi
    • 2
  • Moncef Mrabet
    • 2
    Email author
  1. 1.Faculty of Sciences of TunisUniversity of Tunis El ManarTunisTunisia
  2. 2.Laboratory of Legumes, Centre of Biotechnology of Borj-Cédria (CBBC)Hammam-LifTunisia

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