Effect of mint oil against Botrytis cinerea on table grapes and its possible mechanism of action
This paper assessed the antifungal effects of sage oil, lavender oil, mint oil, and tea tree oil on the postharvest fungal pathogen, Botrytis cinerea, which causes gray molds. The change of morphology, physiological, and biochemical characteristics about fungal hyphae and conidia were determined. As results show, all four oils can effectively inhibit the growth of B.cinerea and the antifungal effects are dose dependent. The best antifungal effect has been found from mint oil. According to in vitro studies, volatile vapor worked better than direct contact. With volatile vapor, the growth of B.cinerea was inhibited completely at 2 for all four oils. Mint oil at 500 μL/L and its volatile vapor at 25 could inhibit both conidia germination and disease incidence significantly in vivo. Observations by using scanning electron microscope and transmission electron microscope revealed that, mint oil could destroy the ultrastructure of hyphae and conidia, resulting in markedly shriveling and crinkling of the hyphae and conidia. It could also thicken and disrupt cell wall, causing cellular nucleic acids and proteins divulged with the damage of the cell wall. The chemical composition analysis of mint oil using GC/MS revealed that its main components were cyclohexanol, cyclohexanone, and some alkenes and alkanes. The majority of the components were effective antifungal agents. The content of volatile cyclohexanol and cyclohexanone were found to be 39.79% and 22.24% respectively.
KeywordsEssential oil Antifungal agent B.Cinerea Grapes
This work was financially supported by National Natural Sciences Foundation of China (31260402, 31460411, 21362028 and 31671904), the Fundamental Research Funds for the Central Universities (GK201603095), Agricultural science and technology innovation and research (2016NY-184 and 2016NY-195), Oversea Scholarship Program of Shaanxi Normal University, and One Hundred Person Project of Shaanxi Province.
Conflict of Interest
The authors declare that they have no conflict of interest.
All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.
This article does not contain any studies with animals performed by any of the authors.
Informed consent was obtained from all individual participants included in the study.
- Adegoke, G. O., Iwahashi, H., Komatsu, Y., Obuchi, K., & Iwahashi, Y. (2000). Inhibition of food spoilage yeasts and aflatoxigenic moulds by monoterpenes of the spice Aframomum danielli. Flavour and Fragrance Journal, 15(3), 147–150. https://doi.org/10.1002/1099-1026(200005/06)15:3<147::AID-FFJ883>3.0.CO;2-0.CrossRefGoogle Scholar
- Aloui, H., Khwaldia, K., Licciardello, F., Mazzaglia, A., Muratore, G., Hamdi, M., & Restuccia, C. (2014). Efficacy of the combined application of chitosan and locust bean gum with different citrus essential oils to control postharvest spoilage caused by Aspergillus flavus in dates. International Journal of Food Microbiology, 170, 21–28.CrossRefPubMedGoogle Scholar
- De Bosch, F. V., Paveley, N., Shaw, M., Hobbelen, P., & Oliver, R. (2011). The dose rate debate: Does the risk of fungicide resistance increase or decrease with dose? Plant Pathology, 60, 597–606.Google Scholar
- Castillo, S., Navarro, D., Zapata, P. J., Guillén, F., Valero, D., Serrano, M., & Martínez-Romero, D. (2010). Antifungal efficacy of Aloevera in vitro and its use as a preharvest treatment to maintain postharvest table grape quality. Postharvest Biology and Technology, 57(3), 183–188.CrossRefGoogle Scholar
- Russo, A., Formisano, C., Rigano, D., Senatore, F., Delfine, S., Cardile, V., Rosselli, S., & Bruno, M. (2013). Chemical composition and anticancer activity of essential oils of Mediterranean sage (Salvia officinalis L.) grown in different environmental conditions. Food and Chemical Toxicology, 55, 42–47.CrossRefPubMedGoogle Scholar
- Sikkema, J., De Bont, J.A., & Poolman, B. (1995). Mechanisms of membrane toxicity of hydrocarbons. Micro Biology and Molecular Biology Reviews, 59(2) 201–222.Google Scholar
- Teper-Bamnolker, P., Dudai, N., Fischer, R., Belausov, E., Zemach, H., Shoseyov, O., & Eshel, D. (2010). Mint essential oil can induce or inhibit potato sprouting by differential alteration of apical meristem. Planta, 232(1), 179–186. https://doi.org/10.1007/s00425-010-1154-5.CrossRefPubMedGoogle Scholar