European Journal of Plant Pathology

, Volume 150, Issue 1, pp 213–222 | Cite as

Isolation and characterization of Zhihengliuella aestuarii B18 suppressing clubroot on Brassica juncea var. tumida Tsen

  • Yuanli Luo
  • Daiwen DongEmail author
  • Zhiqin Gou
  • Xuyi Wang
  • Huan Jiang
  • Yufang Yan
  • Chaojun Wu
  • Changyong ZhouEmail author


Mustard clubroot, caused by Plasmodiophora brassicae, is a serious disease that affects Brassica juncea var. tumida Tsen, a mustard plant that is the raw material for a traditional fermented food manufactured in the Chongqing Municipality, People’s Republic of China. To find antagonistic bacteria for P. brassicae, 124 bacteria were obtained from the rhizosphere soil of B. juncea var. tumida grown in Fuling, Chongqing. Isolates were preliminarily chosen by evaluating the inhibition rate of the P. brassicae resting spore germination. The biocontrol effects of three antagonistic bacteria against clubroot on B. juncea var. tumida were evaluated in a greenhouse experiment. B18 showed the highest control efficiency, at 63.4% in the greenhouse test. In a field trial, B18 was also effective in controlling clubroot, but only at a 49.7% efficiency rate. According to 16S rDNA sequence analysis, strain B18 had a 100% sequence similarity with type strain Zhihengliuella aestuarii DY66T (EU939716). Based on morphological, cultural, physiological and biochemical characteristics, the DNA G + C content, polar lipids, fatty acids, cell wall analysis, as well as DNA–DNA hybridization, strain B18 was identified as Z. aestuarii B18. Thus, the isolate B18 might have a potential biocontrol application for clubroot. We report for the first time that Z. aestuarii B18 can control clubroot.


Antagonistic bacterial Biocontrol Brassica juncea Var. Tumida Tsen Clubroot Plasmodiophora brassicae Zhihengliuella aestuarii 



This work was financially supported by the Natural Science Foundation of Chongqing Science and Technology Commission (grant number: cstc 2013jcyjA80038) and the Special Fund for Post-Doctoral Research Project of Chongqing (grant number: Xm2015046)

Authors’ contributions

CYZ and YLL conceived and designed the study. YLL, DWD, ZQG, HJ, XYW, YFY and CJW collected samples and performed the experiment. YLL carried out the data analysis. YLL and CYZ contributed to the writing of the manuscript.

Compliance with ethical standards

Competing interests

The authors declare that they have no potential conflicts of interest.

Informed consent

All authors read and approved the final manuscript.


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Copyright information

© Koninklijke Nederlandse Planteziektenkundige Vereniging 2017

Authors and Affiliations

  • Yuanli Luo
    • 1
    • 2
  • Daiwen Dong
    • 2
    Email author
  • Zhiqin Gou
    • 2
  • Xuyi Wang
    • 2
  • Huan Jiang
    • 2
  • Yufang Yan
    • 2
  • Chaojun Wu
    • 2
  • Changyong Zhou
    • 1
    Email author
  1. 1.Citrus Research Institute of Chinese Academy of Agricultural ScienceSouthwest UniversityChongqingPeople’s Republic of China
  2. 2.Southeast Chongqing Academy of Agricultural SciencesChongqingPeople’s Republic of China

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