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European Journal of Plant Pathology

, Volume 150, Issue 1, pp 167–178 | Cite as

Association of Pantoea ananatis and Pantoea agglomerans with leaf spot disease on ornamental plants of Araceae Family

  • R. Yazdani
  • N. Safaie
  • M. Shams-BakhshEmail author
Article

Abstract

During a survey in 2011–2012, three ornamental plants of Araceae namely Aglaonema nitidum, Syngonium podophyllum and Dieffenbachia amoena showing foliar disease symptoms were collected from central region of Iran. Infected plants exhibited spots on their leaves which appeared as yellow and water-soaked with chlorotic haloes and necrotic center. To investigate the etiology of this disorder, symptomatic leaves were collected from affected plants and six bacterial strains (B2Y, J3Y, SY, E60Y, E68Y and E5MM) were isolated and identified as Pantoea ananatis or P. agglomerans based on morphological, physiological, biochemical and molecular characters. The pathogenicity tests of the isolates demonstrated that they were not host specific. Furthermore, 16S rRNA gene sequencing revealed that the strains were phylogenetically closely related to genus Pantoea. Multilocus sequence analysis (MLSA) of concatenated partial atpD, gyrB and rpoB gene sequences of the six isolates showed a high similarity of B2Y, J3Y, and SY strains to P. ananatis and also of E60Y, E5MM and E68Y strains to P. agglomerans. These results were confirmed by phylogenetic analysis. To the best of our knowledge, this is the first report of leaf spot and necrosis of A. nitidum, S. podophyllum and D. amoena caused by the genus Pantoea.

Keywords

16S rDNA Bacterial leaf spot Biochemical Pathogenicity 

Notes

Acknowledgements

We gratefully acknowledge financial support from Tarbiat Modares University, Tehran, Iran.

Author contributions

Razieh Yazdani carried out all experiments, drafting the manuscript;

Naser Safaie gave advice;

Masoud Shams-bakhsh supervised the project, conception and design of study, edited the manuscript. All authors read and approved the final manuscript.

Compliance with ethical standards

The result of this work has not been published previously and is not under consideration elsewhere.

Funding

This research was funded by Tarbiat Modares University, Tehran, Iran.

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

10658_2017_1264_MOESM1_ESM.jpg (3.3 mb)
Fig. S1 Bayesian 50% majority rule consensus tree inferred from 90 sequences of partial gyrB under the GTR + I + G model. At nodes are given posterior probabilities values >50%. The new sequences are in bold. (JPEG 3333 kb)
10658_2017_1264_MOESM2_ESM.jpg (3.5 mb)
Fig. S2 Bayesian 50% majority rule consensus tree inferred from 97 sequences of partial rpoB under the GTR + I + G model. At nodes are given posterior probabilities values >50%. The new sequences are in bold. (JPEG 3604 kb)
10658_2017_1264_MOESM3_ESM.jpg (3.2 mb)
Fig. S3 Bayesian 50% majority rule consensus tree inferred from 103 sequences of partial atpD under the GTR + I + G model. At nodes are given posterior probabilities values >50%. The new sequences are in bold. (JPEG 3324 kb)

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Copyright information

© Koninklijke Nederlandse Planteziektenkundige Vereniging 2017

Authors and Affiliations

  1. 1.Department of Plant Pathology, Faculty of AgricultureTarbiat Modares UniversityTehranIran

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