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European Journal of Plant Pathology

, Volume 150, Issue 1, pp 105–113 | Cite as

Development and characterization of EST-derived SSR markers in the cereal cyst nematode Heterodera avenae

  • X. Wang
  • J. Ma
  • H. Liu
  • R. Liu
  • H. LiEmail author
Article

Abstract

The cereal cyst nematode Heterodera avenae is a pathogen of cereal crops and causes high yield losses worldwide. In this study, a collection of 37,348 expressed sequence tags (ESTs) of H. avenae was mined for EST-based simple sequence repeat (SSR) markers, which resulted in the identification of 5604 SSRs. A total of 210 pairs of SSR primers were further developed and used for validation of the amplification rate and assessment of the polymorphism. Eight SSR markers were finally identified and analyzed using 96 individual cysts of H. avenae sampled from three provinces in China. These loci were found to be moderately polymorphic with 3–8 alleles per locus. The observed and expected heterozygosity across the three populations ranged from 0.000 to 0.594 and from 0.000 to 0.731, respectively. The polymorphism information content (PIC) was medium and ranged from 0.080 to 0.562, with a mean of 0.409. The FST ranged from 0.1034 to 0.1550, indicating moderate genetic differentiation among the three H. avenae populations. These EST-SSR markers can be used to study population genetic diversity and the dispersal route of H. avenae in China.

Keywords

Microsatellite markers Phytoparasitic nematode Polymorphism analysis Population genetics 

Notes

Acknowledgements

This work was supported by the Natural Science Foundation of China (Grant No. 31471751) and the Special Fund for Agro-scientific Research in the Public Interest (Grant No. 201503114).

Compliance with ethical standards

Conflict of interest

The authors declare no conflict of interest.

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Copyright information

© Koninklijke Nederlandse Planteziektenkundige Vereniging 2017

Authors and Affiliations

  1. 1.Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Department of Plant PathologyNanjing Agricultural UniversityNanjingPeople’s Republic of China

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