, Volume 28, Issue 9, pp 1115–1125 | Cite as

Impact of insect growth regulators on biology and behavior of Chrysoperla carnea (Stephens) (Neuroptera: Chrysopidae)

  • Mubasshir SohailEmail author
  • Syed Saboor Khan
  • Raza Muhammad
  • Qadeer Ahmed Soomro
  • Muhammad Usman Asif
  • Bhai Khan Solangi


This study investigated the effects of four insect growth regulators (IGRs) on biology and behavior of Chrysoperla carnea. IGRs were sprayed on eggs, larvae (~24-h old), and pupae at recommended doses along with their relatively low and high dose. Eggs, larval, and pupal survival were assessed along with effects on fecundity and fertility of C. carnea adults emerged when pupae were treated. IOBC (International Organization for Biological and Integrated Control) proposed toxicity scale was used to categorize the IGRs. Concerning the eggs lufenuron, pyriproxyfen, and diflubenzuron were categorized as slightly harmful (class 2), whereas buprofezin was categorized as moderately harmful (class 3). Lufenuron and diflubenzuron were classified as slightly harmful (class 2) to C. carnea larvae, while pyriproxyfen and buprofezin were categorized as harmless (class 1). Buprofezin did not affect the locomotion behavior of the larvae as time proportion spent in the treated and untreated zone was equal, while all others were affected significantly. Regarding the pupae, pyriproxyfen and buprofezin were found slightly harmful (class 2) and moderately harmful (class 3), respectively, and considerably reduced fecundity and fertility of the C. carnea adults. Lufenuron and diflubenzuron did not affect significantly when pupae were treated. Based on combined effect, the IGRs lufenuron and diflubenzuron did nott influence the significantly on population parameters comparatively. This could be helpful for the use of IGRs in integration with C. carnea towards their conservation in agroecosystem.


Survival Green lacewing Toxicity Locomotion behavior Biological control 



This work was support by International Atomic Energy Agency under the project No. 18583.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

This article does not describe any studies involving human participants performed by the authors. All applicable international, national and/or institutional guidelines for the care and use of animals were followed.


  1. Agüero F, Arturo M, Neves OJ, Manuel P, Cremonez G, Sergio P (2014) Pyriproxyfen and diflubenzuron effects on the reproduction of Nezara viridula (L.)(Hemiptera: Pentatomidae). Investig Agrar 16:99–106Google Scholar
  2. Bompard A, Jaworski CC, Bearez P, Desneux N (2013) Sharing a predator: can an invasive alien pest affect the predation on a local pest? Popul Ecol 55:433–440CrossRefGoogle Scholar
  3. Bueno A, Freitas S (2004) Effect of the insecticides abamectin and lufenuron on eggs and larvae of Chrysoperla externa under laboratory conditions. BioControl 49:277–283CrossRefGoogle Scholar
  4. Campos MR, Picanco MC, Martins JC, Tomaz AC, Guedes RNC (2011) Insecticide selectivity and behavioral response of the earwig Doru luteipes. Crop Prot 30:1535–1540CrossRefGoogle Scholar
  5. Carvalho GA, Carvalho CF, Souza B, Ulhao JL (2002) Selectivity of insecticides to Chrysoperla externa (Hagen)(Neuroptera: Chrysopidae). Neotrop Entomol 31:615–621CrossRefGoogle Scholar
  6. Chen TY, Liu TX (2002) Susceptibility of immature stages of Chrysoperla rufilabris (Neuroptera: Chrysopidae) to pyriproxyfen, a juvenile hormone analog. J Appl Entomol 126:125–129CrossRefGoogle Scholar
  7. de Castro AA, Legaspi JC, de Souza Tavares W(2018) Lethal and behavioral effects of synthetic and organic insecticides on Spodoptera exigua and its predator Podisus maculiventris PloS ONE 13:e0206789CrossRefGoogle Scholar
  8. Desneux N, Decourtye A, Delpuech JM (2007) The sublethal effects of pesticides on beneficial arthropods. Annu Rev Entomol 52:81–106CrossRefGoogle Scholar
  9. Doane DP, Seward LE (2011) Measuring skewness: a forgotten statistic? J Stat Educ 19:1–18CrossRefGoogle Scholar
  10. Garzón A, Medina P, Amor F, Viñuela E, Budia F (2015) Toxicity and sublethal effects of six insecticides to last instar larvae and adults of the biocontrol agents Chrysoperla carnea (Stephens)(Neuroptera: Chrysopidae) and Adalia bipunctata (L.) (Coleoptera: Coccinellidae). Chemosphere 132:87–93CrossRefGoogle Scholar
  11. Guo W, Wu Z, Song J (2014) Juvenile hormone-receptor complex acts on Mcm4 and Mcm7 to promote polyploidy and vitellogenesis in the migratory locust. PLoS Genet 10:e1004702CrossRefGoogle Scholar
  12. Gusmão MR, Picanço M, Leite GL, Moura MF (2000) Seletividade de inseticidas a predadores de pulgões. Hortic Bras 18:130–133CrossRefGoogle Scholar
  13. He Y, Zhao J, Zheng Y, Desneux N, Wu K (2012) Lethal effect of imidacloprid on the coccinellid predator Serangium japonicum and sublethal effects on predator voracity and on functional response to the whitefly Bemisia tabaci. Ecotoxicology 21:1291–1300CrossRefGoogle Scholar
  14. Hussain D, Ali A, Tariq R, Mushtaq-ul-Hassan M, Saleem M (2012) Comparative toxicity of some new chemistry insecticides on Chrysoperla carnea (Stephens) under laboratory conditions. J Agric Res 50:509–515Google Scholar
  15. Jalali MA, Van Leeuwen T, Tirry L, De Clercq P (2009) Toxicity of selected insecticides to the two-spot ladybird Adalia bipunctata. Phytoparasitica 37:323–326CrossRefGoogle Scholar
  16. Khuhro NH, Biondi A, Desneux N, Zhang L, Zhang Y, Chen H (2014) Trade-off between flight activity and life-history components in Chrysoperla sinica. BioControl 59:19–227CrossRefGoogle Scholar
  17. Liu TX, Chen TY (2000) Effects of the chitin synthesis inhibitor buprofezin on survival and development of immatures of Chrysoperla rufilabris (Neuroptera: Chrysopidae). J Econ Entomol 93:234–239CrossRefGoogle Scholar
  18. Lozowicka B (2015) Health risk for children and adults consuming apples with pesticide residue. Sci Total Environ 502:184–198CrossRefGoogle Scholar
  19. Luna RF, Bestete LR, Torres JB, da Silva-Torres CSA (2018) Predation and behavioral changes in the neotropical lacewing Chrysoperla externa (Hagen) (Neuroptera: Chrysopidae) exposed to lambda-cyhalothrin. Ecotoxicology 27:689–702CrossRefGoogle Scholar
  20. Ma XB, Lin FC, Zhao Y (2015) An adjustment to the Bartlett’s test for small sample size. Commun Stat Simul Comput 44:257–269CrossRefGoogle Scholar
  21. Mansoor MM, Shad SA (2019) Resistance of green lacewing, Chrysoperla carnea (Stephens), to buprofezin: Cross resistance patterns, preliminary mechanism and realized heritability. Biol Control 129:123–127CrossRefGoogle Scholar
  22. Medina P, Budia F, Del Estal P, Vineula E (2003) Effects of three modern insecticides, pyriproxyfen, spinosad and tebufenozide, on survival and reproduction of Chrysoperla carnea adults. Ann Appl Biol 142:55–61CrossRefGoogle Scholar
  23. Mohammadi M, Dastjerdi H, Golmohammadi GR, Hassanpour M (2014) Investigation on the effects of lufenuron and pyridalyl insecticides on the developmental stages of the green lacewing (Chrysoperla carnea Steph.) under laboratory conditions. Plant Prot Sci 1:30–40Google Scholar
  24. Nasreen A, Ashfaq M, Mustafa G, Khan RR (2007) Mortality rates of five commercial insecticides on Chrysoperla carnea (Stephens)(Chrysopidae: Neuroptera). Pak J Agric Sci 44:266–271Google Scholar
  25. Nation S, James L (2015) Insect physiology and biochemistry. CRC Press, New York, NYCrossRefGoogle Scholar
  26. Parliament E (2009) Directive 2009/128/EC of the European Parliament and of the Council of 21 October 2009 establishing a framework for Community action to achieve the sustainable use of pesticides. Off J Eur Union 309:71–86Google Scholar
  27. Pasini RA, Grützmacher AD, de Bastos Pazini J, de Armas FS, Bueno FA, Pires SN (2018) Side effects of insecticides used in wheat crop on eggs and pupae of Chrysoperla externa and Eriopis connexa. Phytoparasitica 46:115–125CrossRefGoogle Scholar
  28. Reddy PP (2016) Selective pesticides in IPM. In: Smith J (ed) Sustainable crop protection under protected cultivation. Springer, Singapore, p 121–131CrossRefGoogle Scholar
  29. Rezaei M, Talebi K, Naveh V, Kavousi A (2007) Impacts of the pesticides imidacloprid, propargite, and pymetrozine on Chrysoperla carnea (Stephens)(Neuroptera: Chrysopidae): IOBC and life table assays. BioControl 52:385–398CrossRefGoogle Scholar
  30. Rimoldi F, Schneider MI, Ronco AE (2008) Susceptibility of Chrysoperla externa eggs (Neuroptera: Chrisopidae) to conventional and biorational insecticides. Environ Entomol 37:1252–1257CrossRefGoogle Scholar
  31. Rugno GR, Zanardi OZ, Parra JRP, Yamamoto PT (2018) Lethal and sublethal toxicity of insecticides to the lacewing Ceraeochrysa Cubana. Neotrop Entomol 48:1–9Google Scholar
  32. Rugno GR, Zanardi OZ, Yamamoto PT (2015) Are the pupae and eggs of the lacewing Ceraeochrysa cubana (Neuroptera: Chrysopidae) tolerant to insecticides? J Econ Entomol 108:2630–2639CrossRefGoogle Scholar
  33. Sohail M, Nasar MH, Muhammad R, Soomro QA, Asif MU, Maari JM (2019) Resistance potential of Chrysoperla carnea (stephens) to insecticides used against sucking complex of cotton. Int J Ecotoxicol Ecobiol 4:1–7CrossRefGoogle Scholar
  34. Sohail M, Soomro QA, Muhammad R, Asif MU, Akbar W, Ismail M (2018) Eggs exposure to low temperature reasons dwindled viability and impeded biology in green lacewing (Chrysoperla carnea Stephen). Int J Biosci 12:206–214Google Scholar
  35. Stark JD, Vargas R, Banks JE (2007) Incorporating ecologically relevant measures of pesticide effect for estimating the compatibility of pesticides and biocontrol agents. J Econ Entomol 100:1027–1032CrossRefGoogle Scholar
  36. Tiwari S, Clayson PJ, Kuhns EH, Stelinski LL (2012) Effects of buprofezin and diflubenzuron on various developmental stages of Asian citrus psyllid, Diaphorina citri. Pest Manag Sci 68:1405–1412CrossRefGoogle Scholar
  37. Castilhos RV, Grützmacher AD, Siqueira PRB, de Moraes ÍL, Gauer CJ (2014) Seletividade de agrotóxicos utilizados em pessegueiro sobre ovos e pupas do predador Chrysoperla externa. Ciênc Rural 44:1921–1928CrossRefGoogle Scholar
  38. Yu C, Fu M, Lin R (2014) Toxic effects of hexaflumuron on the development of Coccinella septempunctata. Environ Sci Pollut R 21:1418–1424CrossRefGoogle Scholar
  39. Zotti MJ, Grutzmacher AD, Lopes IH, Smagghe G (2013) Comparative effects of insecticides with different mechanisms of action on Chrysoperla externa (Neuroptera: Chrysopidae): Lethal, sublethal and dose–response effects. Insect Sci 20:43–752Google Scholar

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© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Plant Protection DivisionNuclear Institute of AgricultureTando JamPakistan
  2. 2.Department of Plant ProtectionSindh Agriculture UniversityTando JamPakistan

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