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Acute toxicity of copper to the larval stage of three species of ambystomatid salamanders

  • Scott M. WeirEmail author
  • Shuangying Yu
  • David E. Scott
  • Stacey L. Lance
Article

Abstract

Copper (Cu) appears to be consistently more toxic to anuran species relative to other vertebrate taxa. There are limited Cu toxicity data for salamanders; of the few studies conducted on salamanders, most examined Cu effects on the embryonic, but not the larval, stage. We performed acute toxicity experiments, to quantify LC50s, on Harrison stage 46 larvae (free swimming hatchlings with egg yolk completely absorbed) of three ambystomatid salamander species. Each LC50 experiment used exposure concentrations of 10, 20, 30, 40, 50, and 60 µg/L with 10 replicates per concentration each containing one larva. We found very high toxicity for all species compared to previously published research on the embryo stage. Specifically, the 4-d LC50s for Ambystoma tigrinum and A. opacum were 35.3 and 18.73 µg/L, respectively. The same Cu concentrations caused similar toxicity to A. talpoideum (LC50 = 47.88 µg/L), but exposures required up to 48 d to elicit the same level of mortality. A time-to-event analysis indicated that time to mortality was significantly affected by Cu concentration. Additionally, for A. talpoideum, we observed that elevated levels of Cu decreased growth rate. Comparisons with previously reported Cu toxicity for embryos suggest that, as with fish, Cu may be more toxic to larval salamander stages than for embryos. Further, our data suggest that Cu is an important environmental contaminant that deserves increased scrutiny on the potential for population-level effects where contamination has occurred in wetlands and streams inhabited by salamanders.

Keywords

Amphibians Metals Mortality LC50 Caudata Sublethal effect 

Notes

Acknowledgements

We thank A. Coleman for assistance in the lab and field collections. Animals were collected under SCDNR permit #G-09-03 following IACUC procedures (A2012-12-003-Y3-A3) from the University of Georgia. We thank three anonymous reviewers for comments that improved the manuscript.

Funding

This research was partially supported by United States Department of Energy under Award Numbers DE­FC09­07SR22506 and DE-EM0004391 to the University of Georgia Research Foundation, and was also made possible by the status of the Savannah River Site as a National Environmental Research Park, as well as the protection of research wetlands in the SRS Set-Aside Program. Project funding was provided by the DOE National Nuclear Security Administration.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

All applicable international, national, and/or institutional guidelines for the care and use of animals were followed. All procedures performed in studies involving animals were in accordance with the ethical standards of the institution or practice at which the studies were conducted.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Scott M. Weir
    • 1
    • 2
    Email author
  • Shuangying Yu
    • 1
    • 3
  • David E. Scott
    • 1
  • Stacey L. Lance
    • 1
  1. 1.Savannah River Ecology LaboratoryUniversity of GeorgiaAikenUSA
  2. 2.Department of BiologyQueens University of CharlotteCharlotteUSA
  3. 3.Sciences DivisionCentral Piedmont Community CollegeCharlotteUSA

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