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Acute exposure to urban air pollution impairs olfactory learning and memory in honeybees

  • Ryan J. LeonardEmail author
  • Thomas J. Pettit
  • Peter Irga
  • Clare McArthur
  • Dieter F. Hochuli
Article

Abstract

While the ecological effects of pesticides have been well studied in honeybees, it is unclear to what extent other anthropogenic contaminants such as air pollution may also negatively affect bee cognition and behaviour. To answer this question, we assessed the impacts of acute exposure to four ecologically relevant concentrations of a common urban air pollutant—diesel generated air pollution on honeybee odour learning and memory using a conditioned proboscis extension response assay. The proportion of bees that successfully learnt odours following direct air pollution exposure was significantly lower in bees exposed to low, medium and high air pollutant concentrations, than in bees exposed to current ambient levels. Furthermore, short- and long-term odour memory was significantly impaired in bees exposed to low medium and high air pollutant concentrations than in bees exposed to current ambient levels. These results demonstrate a clear and direct cognitive cost of air pollution. Given learning and memory play significant roles in foraging, we suggest air pollution will have increasing negative impacts on the ecosystem services bees provide and may add to the current threats such as pesticides, mites and disease affecting colony fitness.

Keywords

Air pollution Honeybee Pollinator Proboscis extension reflex response Behaviour 

Notes

Acknowledgements

The authors thank T. Latty and C. Perry for feedback during manuscript writing.

Funding

Authors (RJL, TJP, PI, CM and DFH) received no funding for this project.

Authors’ contributions

RJL, TP, PI, CM and DFH conceived the project; RJL collected the data; TP and PI conducted chemical. analyses; RJL, TP, CM and DF wrote the manuscript. All authors gave final approval for publication.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

All applicable international, national, and/or institutional guidelines for the care and use of animals were followed.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Ryan J. Leonard
    • 1
    Email author
  • Thomas J. Pettit
    • 2
  • Peter Irga
    • 3
  • Clare McArthur
    • 1
  • Dieter F. Hochuli
    • 1
  1. 1.School of Life and Environmental SciencesThe University of SydneySydneyAustralia
  2. 2.School of Life SciencesUniversity of Technology SydneySydneyAustralia
  3. 3.School of Civil and Environmental EngineeringUniversity of Technology SydneySydneyAustralia

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