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Ecotoxicology

, Volume 28, Issue 7, pp 763–770 | Cite as

Cytotoxicity of Piper aduncum (Piperaceae) essential oil in brown stink bug Euschistus heros (Heteroptera: Pentatomidae)

  • Jamile F. S. Cossolin
  • Mônica J. B. Pereira
  • Luis C. Martínez
  • Leonardo M. Turchen
  • Muhammad Fiaz
  • Hakan Bozdoğan
  • José Eduardo SerrãoEmail author
Article

Abstract

Euschistus heros (F.) (Hemiptera: Pentatomidae) is a soybean pest in Brazil, controlled with synthetic chemical insecticides, which may be harmful to the environment and humans, as well as to select pest resistant strains. The research for new pest control strategies such as the use of plant essential oils has been increased due to the selectivity and biodegradation of these molecules. The objective was to evaluate the cytological changes in the salivary glands, fat body and midgut of E. heros exposed to different concentrations of essential oil of Piper aduncum L. (Piperales: Piperaceae), which the main compounds were identified as myristicin 30.03%, aromadendrene 9.20%, dillapiole 8.43%, α-serinene 7.31%, tridecane 6.26%, γ-elemene 4.58% and o-cymene 4.20%. The essential oil of P. aduncum was toxic for E. heros with LD50 = 36.23 mg per insect and LD90 = 50.42 mg per insect. Cytological changes such as tissue disruption, increase in mitochondria population, and glycogen and lipid depletion occur in the fat body cells, whereas salivary glands and midgut are not affected by this essential oil. Results suggest that P. aduncum essential oil causes fat body cellular stress, which may compromise some physiological processes for the insect survival.

Keywords

Botanical insecticide Fat body Midgut Pest control Salivary glands Toxicity 

Notes

Acknowledgements

We thank to Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) (Brazil), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) (Brazil), Fundação de Amparo à Pesquisa do Estado de Mato Grosso (FAPEMAT) (Brazil), Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG) (Brazil), and Núcleo de Microscopia e Microanálise from Universidade Federal de Viçosa.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

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© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Departament of General BiologyUniversidade Federal de ViçosaViçosaBrazil
  2. 2.Departament of AgronomyUniversidade Estadual do Mato GrossoTangará da SerraBrazil
  3. 3.Departament of EntomologyUniversidade Federal de ViçosaViçosaBrazil
  4. 4.Department of Plant and Animal Production, Kirsehir Vocational School of Technicial SciencesKirsehir Ahi Evran UniversityKirsehirTurkey

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