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Ecotoxicology

, Volume 28, Issue 7, pp 744–753 | Cite as

Oral acute toxicity and impact of neonicotinoids on Apis mellifera L. and Scaptotrigona postica Latreille (Hymenoptera: Apidae)

  • Cynthia R. O. JacobEmail author
  • José B. Malaquias
  • Odimar Z. Zanardi
  • Carina A. S. Silva
  • Jessica F. O. Jacob
  • Pedro T. Yamamoto
Article

Abstract

Wild and managed bees are essential for crop pollination and food production. However, the widespread use of insecticides such as neonicotinoids may affect the survival, development, behavior, and maintenance of bee colonies. Therefore, in this study we evaluated the impacts of three neonicotinoid insecticides on the survival and walking abilities of the Africanized honeybee A. mellifera and stingless bee S. postica. A. mellifera was more susceptible than S. postica to all neonicotinoids tested. The median lethal concentrations LC50 values estimated for acetamiprid, imidacloprid, and thiacloprid were 189.62, 22.78, and 142.31 ng µL–1 of diet for A. mellifera, and 475.94, 89.11, and 218.21 ng µL–1 of diet for S. postica, respectively. All tested neonicotinoids affected the speed, distance traveled, duration and frequency of resting, and continuous mobility of both bee species. The results showed that in spite of the different susceptibility to compounds with cyano and nitro radicals, the behavioral variables showed different levels of commitment according to the molecule insecticide and bee species. These results contribute not only to the understanding of the effects of neonicotinoid insecticides on A. mellifera and S. postica, but also to help in the development of protocols that aim to reduce the impact of these insecticides in Neotropical environments.

Keywords

Africanized honeybee Stingless bee Susceptibility Motor impairment Conservation 

Notes

Acknowledgements

The authors gratefully acknowledge the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior—Brasil (CAPES)—Finance Code 001, for financial support and scholarships. The authors thank Paulo César Candelori for assistance with the collect A. mellifera combs and are grateful to Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP), for the resources provided to the Laboratory of Insect Ecology (FAPESP process: 2014/16607-9) and for the second author (FAPESP process: 2018/20435-5, 2017/05953-7, 2015/20380-8).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors. The authors agree with the publication of the manuscript in this form.

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Authors and Affiliations

  1. 1.Department of Entomology and Acarology“Luiz de Queiroz” College of Agriculture/University of São Paulo (ESALQ/USP)PiracicabaBrazil
  2. 2.Department of EntomologyFund for Citrus Protection (FUNDECITRUS)AraraquaraBrazil

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