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Involvement of glutamate receptors in regulating calcium influx in rice seedlings under Cr exposure

  • Yan-Hong Li
  • Xiao-Zhang YuEmail author
  • Ling-Yun Mo
  • Yu-Juan Lin
  • Qing Zhang
Article

Abstract

Glutamate receptors (GLRs) are ligand-gated Ca2+-permeable channels that govern and modulate the dynamic influx of cytosolic Ca2+ in plants. The present study investigated the interaction of OsGLR3 gene expression with subcellular Ca distribution in rice seedlings exposed to chromium (Cr) solution containing Cr(III) or Cr(VI). The results displayed that the accumulation of Ca was evaluated or higher in shoots compared to roots under Cr exposure, and a similar pattern of subcellular Ca distribution was observed between rice tissues exposed to Cr(III) and Cr(VI). Real-time quantitative polymerase chain reaction (qRT-PCR) analysis revealed that eight OsGLR3 isogenes were distinctly expressed in different rice tissues at different levels of Cr exposures. This differential expressions could possible be due to the uptake variations, subcellular distribution and chemical speciation of the two Cr species. Notably, distinct expression patterns of OsGLR3 genes were found between Cr(III) and Cr(VI) exposures, suggesting that different regulation strategies are used to mediate Ca influx in rice materials under different Cr exposures. These results demonstrated a full picture of Cr-induced transcriptional alterations in OsGLR3 expression levels in rice seedlings, and provided suggestive evidence for further investigation on specific OsGLR3 genes participated in the regulation of cytosolic Ca2+ concentrations under Cr exposure.

Keywords

Calcium Chromium Glutamate receptors PCR Rice Subcellular distribution 

Notes

Acknowledgements

This work was financially supported by the Natural Science Foundation of Guangxi (No: 2018GXNSFDA281024).

Compliance with ethical standards

Conflict of interest

X-Z Yu has received the grants from the Natural Science Foundation of Guangxi. The other authors, Y-H Li, L-Y Mo, Y-J Lin, Q Zhang declare that they have no conflict of interest.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.

Supplementary material

10646_2019_2062_MOESM1_ESM.doc (44.4 mb)
Suppl Mtrls (Fig. S1–2)

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Yan-Hong Li
    • 1
  • Xiao-Zhang Yu
    • 1
    Email author
  • Ling-Yun Mo
    • 1
  • Yu-Juan Lin
    • 1
  • Qing Zhang
    • 1
  1. 1.College of Environmental Science & EngineeringGuilin University of TechnologyGuilinChina

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