Comparative study of phytotoxicity and genotoxicity of soil contaminated with biodiesel, diesel fuel and petroleum
The worldwide spillage of fossil fuels causes an ever-increasing environmental concern due to their resistance to biodegradation and toxicity. The diesel fuel is one of the derivative forms of petroleum that is widely used in the world. Its composition has many aromatic compounds and long hydrocarbons chains, both persistent and hazardous, thus requiring complex microbial dynamics to achieve full biodegradation. At this point, biodiesel has advantages because it is produced from renewable sources. It also has a relatively fast biodegradation. Biodiesel formulation chemically varies according to the raw material used for its production. While vegetable oils tend to have homogeneous proportions of linoleic and oleic fatty acids, animal fats have an heterogeneous distribution of stearic, palmitic and oleic fatty acids. As some studies have already detected the toxic potential of biodiesel from vegetable oil, this study sought information on the phytotoxic and genotoxic potential of animal fat-based biodiesel and compare it with fossil fuel as diesel fuel and crude petroleum. The impacts on the microbial activity of soils contaminated with biodiesel, diesel fuel and crude petroleum were performed by the dehydrogenase activity. Phytotoxicity tests were performed with Eruca sativa seeds and genotoxicity bioassays with Allium cepa seeds. The results showed a rapid assimilation of biodiesel by the autochthonous soil microorganisms. Soil contaminated with either diesel or crude petroleum inhibited the root and hypocotyl elongation of E. sativa. Overall, petroleum contaminated soils showed higher genotoxic potential. Biodiesel from animal fat was rapidly assimilated by soil microorganisms and did not present significant phytotoxic or genotoxic potential, but significantly reduced the mitotic index of A. cepa roots. Our results showed that biodiesel from animal fat have rapid biodegradability. Biodiesel also led to less impacts during seed development and lower genotoxic potential when compared to crude petroleum and diesel fuel. In addition, biodiesel from animal fat does not present the same toxicity demonstrated by biodiesel from soybean-based biodiesel described in current literature.
KeywordsAllium cepa Soil dehydrogenase activity Biodegradation Animal fat biofuel Hydrocarbons Chromosomal abnormalities
This study was financially supported by FAPESP [Grant number 2013/13813-0].
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
This article does not contain any studies with humans or with animals participants performed by any of the authors.
- Alef K (1995) Dehydrogenase activity. In: Alef K, Nannipieri P (ed) Methods in applied soil microbiology and biochemistry, 1st edn. Academic Press, Michigan, p 228–231Google Scholar
- Bamgbose IA, Anderson TA (2015) Ecotoxicology and environmental safety phytotoxicity of three plant-based biodiesels, unmodified castor oil, and diesel fuel to alfalfa (Medicago sativa L.), lettuce (Lactuca sativa L.), radish (Raphanus sativus), and wheatgrass (Triticum aesti). Ecotoxicol Environ Saf 122:268–274. https://doi.org/10.1016/j.ecoenv.2015.08.003 CrossRefGoogle Scholar
- Chabauty F, Pot V, Bourdat-Deschamps M, Bernet N, Labat C, Benoit P (2016) Transport of organic contaminants in subsoil horizons and effects of dissolved organic matter related to organic waste recycling practices. Environ Sci Pollut Res 23:6907–6918. https://doi.org/10.1007/s11356-015-5938-9 CrossRefGoogle Scholar
- EIA-U.S. Energy Information Administration (2017) Monthly biodiesel production report. Washington, DC. https://www.eia.gov/biofuels/biodiesel/production/biodiesel.pdf
- Guarino C, Spada V, Sciarrillo R (2017) Assessment of three approaches of bioremediation (Natural Attenuation, Landfarming and Bioagumentation e Assistited Landfarming) for a petroleum hydrocarbons contaminated soil. Chemosphere 170:10–16. https://doi.org/10.1016/j.chemosphere.2016.11.165 CrossRefGoogle Scholar
- Lahel A, Fanta AB, Sergienko N, Shakya M, Estefanía ML, Behera SK, Rene ER, Park H (2016) Effect of process parameters on the bioremediation of diesel contaminated soil by mixed microbial consortia. Int Biodeterior Biodegrad 113:375–385. https://doi.org/10.1016/j.ibiod.2016.05.005 CrossRefGoogle Scholar
- Leme DM, Grummt T, Palma D, Oliveira D, Sehr A, Renz S, Reinel S, Ferraz ERA, Rosa M, Marchi R, Canto M, Julião G, Marin-morales MA (2012) Genotoxicity assessment of water soluble fractions of biodiesel and its diesel blends using the Salmonella assay and the in vitro MicroFlow Ò kit (Litron) assay. Chemosphere 86:512–520. https://doi.org/10.1016/j.chemosphere.2011.10.017 CrossRefGoogle Scholar
- Njoku KL, Akinola MO, Ige TO (2009a) Comparative effects of diesel fuel and spent lubricating oil on the growth of Zea mays (maize). Am Eurasia J Sustain Agric 3:428–434Google Scholar
- Njoku KL, Akinola MO, Taiwo BG (2009b) Effect of gasoline diesel fuel mixture on the germination and the growth of Vigna unguiculata (Cowpea). Afr J Environ Sci Technol 3:12Google Scholar
- OECD-Guideline for the testing of chemicals (2003) Terrestrial plant test: 208: Seedling emergence and seedling growth test. http://www.oecd.org/chemicalsafety/testing/33653757.pdf
- Pereira SA, Araújo VQ, Reboucas MV, Vieira FSV, De Almeida MVA, Chinalia FA, Nascimento IA (2012) Toxicity of biodiesel, diesel and biodiesel/diesel blends: Comparative sub-lethal effects of water-soluble fractions to microalgae species. Bull Environ Contam Toxicol 88:234–238. https://doi.org/10.1007/s00128-011-0430-9 CrossRefGoogle Scholar
- Sobrero MC, Ronco A (2004) Ensayo de toxicidad aguda con semillas de lechuga Lactuca sativa. In: Castillo G (ed.) Ensayos toxicológicos y métodos de evaluación de calidad de aguas. Estandarización, intercalibración, resultados y aplicaciones. IDRC, IMTA, Canadá, p 202Google Scholar
- Souza TS, Hencklein FA, Angelis DF, Gonçalves RA, Fontanetti CS (2009) The Allium cepa bioassay to evaluate landfarming soil, before and after the addition of rice hulls to accelerate organic pollutants biodegradation. Ecotoxicol Environ Saf 72:1363–1368. https://doi.org/10.1016/j.ecoenv.2009.01.009 CrossRefGoogle Scholar
- U.S. EPA-United States Environmental Protection Agency (1996) Ecological effects test guidelines: seed germination/root elongation toxicity test.Google Scholar