Environmental Biology of Fishes

, Volume 102, Issue 8, pp 1057–1068 | Cite as

Have a break or keep going - behavioral and metabolic overwintering strategies of two invasive species of the river Rhine, Germany

  • Rodrigo Fortes Silva
  • Katja Heubel
  • Marie-Victoria Simon
  • Jost BorcherdingEmail author


Winter conditions, extended periods of low temperature and limited food availability, impose pressures to develop strategies to cope with such unfavorable environmental conditions. Overwintering strategies may embrace both behavioral and physiological adaptations and result in differential responses. To address the effects of low temperature and feeding treatments (feeding or starving) in two common invasive goby species of the River Rhine, the round goby (Neogobius melanostomus) and the monkey goby (Neogobius fluviatilis) we monitored locomotor activity and measured oxygen consumption at 5 °C in monthly intervals for 90 days. Under still warm water conditions at 15 °C as a baseline, locomotor activity did not differ between the two species, but N. melanostomus had a higher metabolic rate. During the course of the 5 °C cold overwintering period, the behavioral responses were strikingly different between the two species: N. melanostomus maintained higher activity levels regardless of the feeding treatment, while N. fluviatilis showed a lower activity with a strong response to the feeding treatment. After 90 days of simulated winter condition, the starved groups of both species increased their locomotor activity. Oxygen consumption on the other hand showed no differences between fed and starved N. fluviatilis. In contrast, N. melanostomus had a stronger response to temperature per se and for starving individuals consistently lower metabolic rates. We conclude that the two locally most abundant fish species adopted two different overwintering strategies. N. fluviatilis applies a low energy expenditure strategy resulting in a high survival rate even under starvation. N. melanostomus applies a more risky high energy expenditure strategy with increased activity levels under extended periods of starvation.


Energetic costs Temperature Oxygen consumption Gobiidae Coping style Competition 



Thanks are due to our colleagues Svenja Storm, Sebastian Wantia, and Lara Reinartz for their help with fieldwork, to Laura Mehner for oxygen measurement suggestions. We appreciate the time spent by two anonymous referees on revising this manuscript and thank them for their extensive and useful comments which strengthened this manuscript substantially. To CNPq by Brazilian Government in collaboration with German Academic Exchange Service (DAAD) for granting post-doctoral scholarships to R. Fortes-Silva (number 205749/2014-3). The study was supported by an internal grant from the University of Köln/Germany to JB and a grant from the Volkswagen foundation (# 92 002) to KH.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

All applicable international, national, and/or institutional guidelines for the care and use of animals were followed. All procedures performed in studies involving animals were in accordance with the ethical standards of the institution or practice at which the studies were conducted. This article does not contain any studies with human participants performed by any of the authors.

Supplementary material

10641_2019_890_MOESM1_ESM.pdf (65 kb)
ESM 1 (PDF 65.2 kb)


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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  • Rodrigo Fortes Silva
    • 1
    • 2
  • Katja Heubel
    • 1
    • 3
  • Marie-Victoria Simon
    • 1
  • Jost Borcherding
    • 1
    Email author
  1. 1.Institute for Zoology, General Ecology & Limnology, Ecological Research Station ReesUniversity of CologneCologneGermany
  2. 2.Laboratory of Feeding Behavior and Fish Nutrition (AquaUFRB), Faculty of Fish Engineering (NEPA), Center of Agricultural Sciences, Environmental and Biological (CCAAB)Campus Cruz das Almas, Federal University of Bahia (UFRB)BahiaBrazil
  3. 3.Research and Technology Centre (FTZ)Kiel UniversityBüsumGermany

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