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7-Chloroquinoline-1,2,3-triazoyl carboxamides induce cell cycle arrest and apoptosis in human bladder carcinoma cells

  • Mariana S. Sonego
  • Natália V. Segatto
  • Lucas Damé
  • Mariana Fronza
  • Carolina B. Gomes
  • Thais Larré Oliveira
  • Fabiana Kömmling Seixas
  • Lucielli Savegnago
  • Kyle M. Schachtschneider
  • Diego Alves
  • Tiago CollaresEmail author
PRECLINICAL STUDIES
  • 16 Downloads

Summary

In the present study, the antitumoral properties of a series of 7-chloroquinoline-1,2,3-triazoyl-carboxamides (QTCA) were investigated by analyzing their cytotoxic activities against human bladder cells (5637; grade II carcinoma). In addition, their effects on cell viability, cell cycle arrest mechanisms, apoptosis induction, in silico molecular docking, and detection of pro-apoptotic and anti-apoptotic proteins were evaluated. The cytotoxicity assay identified major dose- and time-dependent cytotoxic effects in 5637 cells after they were exposed to treatment with QTCA, only minimal effects were observed on normal cells. A live/dead assay confirmed that significant cell death, arrest in the G0/G1 phase and apoptosis were associated with treatment by 1-(7-Chloroquinolin-4-yl)-5-methyl-N-phenyl-1H-1,2,3-triazole-4-carboxamide (QTCA-1) and 1-(7-Chloroquinolin-4-yl)-N-(4-fluorophenyl)-5-methyl-1H-1,2,3-triazole-4-carboxamide (QTCA-4). The in silico results indicated that these compounds acted through different mechanisms for the induction of cell cycle arrest and apoptosis. Western blotting confirmed the binding of the QTCAs to pro- and anti-apoptotic proteins. In conclusion, QTCA-1 and QTCA-4 are promising candidates for inducing cytotoxicity, cell cycle arrest, and apoptosis in human bladder cancer cells.

Keywords

Anticancer Bladder cancer cytotoxicity Quinoline derivatives Apoptosis Cell cycle arrest 

Notes

Author contributions

Material preparation, data collection and analysis were performed by Mariana S. Sonego, Natália V. Segatto, Lucas Damé, Mariana Fronza, Carolina B. Gomes, Thais Larré Oliveira. Study conception and design were supervised by Fabiana Kömmling Seixas, Lucielli Savegnago, Kyle M. Schachtschneider, Diego Alves and Tiago Collares. The first draft of the manuscript was written by Mariana S. Sonego and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.

Funding information

This study was financed in part by the Coordenação de Aperfeiçoamento de Pessoal de Nivel Superior - Brasil (CAPES)–Finance Code 001.

Compliance with ethical standards

Conflict of interest

The authors declare that there is no conflict of interest regarding the publication of this article.

Ethical approval

This article does not contain any studies involving the use of human participants or animals.

Informed consent

For this type of study, formal consent is not required.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Mariana S. Sonego
    • 1
  • Natália V. Segatto
    • 1
  • Lucas Damé
    • 1
  • Mariana Fronza
    • 2
  • Carolina B. Gomes
    • 3
  • Thais Larré Oliveira
    • 1
  • Fabiana Kömmling Seixas
    • 1
  • Lucielli Savegnago
    • 2
  • Kyle M. Schachtschneider
    • 4
    • 5
    • 6
  • Diego Alves
    • 3
  • Tiago Collares
    • 1
    Email author
  1. 1.Laboratório de Biotecnologia do Câncer, Programa de Pós-Graduação em Biotecnologia (PPGB), Centro de Desenvolvimento Tecnológico (CDTec)Universidade Federal de PelotasCapão do LeãoBrazil
  2. 2.Grupo de Pesquisa em Neurobiotecnologia (GPN), Centro de Desenvolvimento Tecnológico (CDTec)Universidade Federal de PelotasPelotasBrazil
  3. 3.Laboratório de Síntese Orgânica Limpa (LASOL), CCQFAUniversidade Federal de PelotasCapão do LeãoBrazil
  4. 4.Department of RadiologyUniversity of Illinois at ChicagoChicagoUSA
  5. 5.Department of Biochemistry & Molecular GeneticsUniversity of Illinois at ChicagoChicagoUSA
  6. 6.National Center for Supercomputing ApplicationsUniversity of Illinois at Urbana-ChampaignUrbanaUSA

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