Transplantation of rat embryonic stem cell-derived retinal cells restores visual function in the Royal College of Surgeons rats
Aim of study
To evaluate the feasibility of transplantation of embryonic stem cell (ESC)-derived retinal cells in the treatment of retinal degeneration.
Materials and methods
Rat ESCs were isolated and induced into retinal progenitor cells (RPCs) in vitro, which were subsequently induced into retinal pigment epithelium cells (RPEs) and photoreceptors (PRCs). All cells were identified by Western blot detection of their specific markers. RPEs and PRCs were, respectively, injected into the retina of Royal College of Surgeons (RCSs) rats. Control group was injected with PBS. Post-transplantation visual function was determined by electroretinography (ERG). The histology of the whole eye was compared by H&E staining.
RPEs and PRCs were successfully derived from rat ESCs through the two-step differentiation as indicated by the presence of ESC- (Oct-3/4, Nanog, TRA-1-60 and TRA-1-81), RPC- (Rx, Mitf, Pax6 and Chx10), RPE- (RPE65 and keratin) and PRC-specific markers (blue opsin, red/green opsin, recoverin and rhodopsin) in Western blot. The amplitude of ERG a- and b-wave in RPE- and PRC-transplanted groups at week 2 and 10 after transplantation was markedly higher compared with PBS controls. Retinal injury and vascular stress response was not detected in any of the RCS rats after transplantation.
The developed stepwise protocol can derive retinal cells from ESCs. Transplantation of these retinal cells can restore visual function of RCS rats. Our study provides evidence for potential clinical application of ESC-based cell therapy for retinal degeneration.
KeywordsRetinal degenerative diseases Embryonic stem cell Retinal progenitor cell Retinal pigment epithelium cell Photoreceptor RCS rats
This study was supported by Scientific and Technological Landing Project of Higher Education in Jiangxi Province (12004044).
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
Statement on the welfare of animals
This study was approved by the Research Ethics Committee at the Second Affiliated Hospital of Nanchang University, and was performed in strict accordance with the University's ethical standards.
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