Down-Regulation of LINC00460 Represses Metastasis of Colorectal Cancer via WWC2

  • Bao Yuan
  • Jing Yang
  • Hong Gu
  • Chaoqun MaEmail author
Original Article



Colorectal cancer (CRC) is one of the most prevalent cancers and a common cause of cancer-related death. Long noncoding RNAs have been reported to play an essential role in the development of CRC.


This study aimed to investigate the possible function of LINC00460 in CRC.


Initially, microarray-based gene expression profiling of CRC was employed to identify differentially expressed genes. Next, the expression of LINC00460 was examined and the cell line presenting with the highest LINC00460 expression was selected for subsequent experimentation. Then, the interaction among LINC00460, ERG, and WWC2 was identified. The effect of LINC00460 on proliferation, migration, invasion, and epithelial–mesenchymal transition (EMT)-related factors as well as tumorigenicity of transfected cells was examined with gain- and loss-of-function experiments.


LINC00460 was robustly induced while WWC2 was poorly expressed in CRC. In addition, LINC00460 could down-regulate WWC2 through interaction with ERG, which led to promoted invasion, migration, and EMT of CRC cells in addition to tumor growth in vivo. Besides, down-regulation of LINC00460 exerted inhibitory effect on these biological activities.


Taken together, the key findings of the current study provided evidence suggesting that silencing of LINC00460 could potentially suppress EMT of CRC cells by increasing WWC2 via ERG, and highlighting that knockdown of LINC00460 could serve as a therapeutic target for CRC treatment.


LINC00460 WWC2 Colorectal cancer Migration Epithelial–mesenchymal transition 



We acknowledge and appreciate our colleagues for their valuable efforts and comments on this paper.

Author’s contribution

Jing Yang designed the study. Chaoqun Ma and Hong Gu collated the data, designed and developed the database, carried out data analyses, and produced the initial draft of the manuscript. Bao Yuan contributed to drafting the manuscript. All authors have read and approved the final submitted manuscript.

Compliance with Ethical Standards

Conflict of interests

They authors declare that they have no conflict of interests.

Ethics statement

The current study was approved by the Ethics Committee of Affiliated Hospital of Nanjing University of Chinese Medicine. Signed informed consents were obtained from all participants. Nude mice were enrolled for in vivo studies and were cared for according to the Guide for the Care and Use of Laboratory Animals published by the National Institutes of Health. All efforts were made to minimize the suffering of the included animals.

Supplementary material

10620_2019_5801_MOESM1_ESM.eps (6.2 mb)
Supplementary Fig. 1 LINC00460 over-expression promotes proliferation, migration, invasion, and EMT of LoVo cells. A, expression patterns of LINC00460 in LoVo cells treated with over-expressed or silenced LINC00460 detected by RT-qPCR. B, the proliferation of LoVo cells treated with over-expressed or silenced LINC00460 detected by EdU assay. C, the migration of LoVo cells treated with over-expressed or silenced LINC00460 detected by Transwell assay. D, the invasion of LoVo cells treated with over-expressed or silenced LINC00460 detected by Transwell assay. In panel A–D, * p < 0.05 vs. the blank group or the GapmeR-NC group. E, Western blot analysis of E-cadherin, N-cadherin, Snail, and TWSIT1 protein patterns in LoVo cells treated with over-expressed or silenced LINC00460. The above data were all measurement data and expressed as mean ± standard error. Data among multiple groups were compared by one-way ANOVA. The experiment was repeated three times independently. (EPS 6341 kb)


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of Anorectal SurgeryJiangyin Hospital Affiliated to Nanjing University of Chinese MedicineJiangyinPeople’s Republic of China
  2. 2.Department of General SurgeryJiangyin Hospital Affiliated to Nanjing University of Chinese MedicineJiangyinPeople’s Republic of China
  3. 3.Department of General SurgeryAffiliated Hospital of Nanjing University of Chinese MedicineNanjingPeople’s Republic of China

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