miR-9-5p Suppresses Malignant Biological Behaviors of Human Gastric Cancer Cells by Negative Regulation of TNFAIP8L3
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MicroRNA is essential for the malignant progression of human gastric cancer (GC), which is a leading cause of cancer deaths. However, the mechanism is still not so clear.
In our present research, we investigated the effect of miR-9-5p in GC.
We detected miR-9-5p expression in human gastric epithelial cell (GES-1) and GC cells (AGS, BGC-823, MKN-45, and MGC-803), plasma of normal or GC patients, as well as orthotopic xenograft mouse models by real-time PCR. The migration ability was detected by Transwell assays after miR-9-5p mimic or inhibitor transfection in GC cells.
Our results showed that miR-9-5p expression in GC cells and plasma was significantly decreased. miR-9-5p inhibited migration of GC cells by regulating TNFAIP8L3 directly. Low expression of miR-9-5p in GC patients hardly suppressed the migration mediated by TNFAIP8L3.
miR-9-5p, as a potential tumor suppressor gene, is closely related to the malignant progression of GC. Exploring the regulation between miR-9-5p and TNFAIP8L3 may provide a novel strategy for GC treatment.
KeywordsmiR-9-5p TNFAIP8L3 Gastric cancer Migration 3′UTR
This research was supported by the Youth Research Project of Health and Family Planning Commission of Fujian Province, China (No. 2015-2-48), Natural Science Foundation of Guangdong Province, China (No. 2018A0303130302), Medical Research Fund of Guangdong Province, China (No. A2018011), and Science Foundation for The Excellent Youth Scholars of Jinan University, China (Nos. 89018021 and 21618302).
WML, GHS, and DYS conceived the project. WML and GHS designed the experiments; YYF, YS, ZHL, XXH, JYL, and WH performed the experiments; and YS and WML wrote the paper.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
Research involving human participants and/or animals and informed consent
All samples were obtained with patients’ informed consent. The Ethics Committee of Zhongshan Hospital, Xiamen University and The First Affiliated Hospital, Jinan University approved this study. This study does not involve animals.