WDR34 Activates Wnt/Beta-Catenin Signaling in Hepatocellular Carcinoma
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Wnt ligand binding initiates the interaction between Frizzled and Dvl proteins. However, the regulation of Frizzled–Dvl proteins interaction remains largely unknown.
The present study aims to elucidate the regulation of Frizzled–Dvl interaction by WDR34.
The protein levels of WDR34 in hepatocellular carcinoma (HCC) tissues were examined by western blot and immunohistochemistry. The effects of WDR34 on the growth and migration of HCC cells were examined using MTT assay and Boyden chamber assay. The interaction between Frizzled and Dvl was evaluated by immunoprecipitation and GST pull-down assay.
In this study, we have shown that WDR34, the binding protein of Frizzled (Fz) activated beta-catenin/TCF signaling by enhancing the interaction between Fz and Dvl2. WDR34 was found to up-regulate in HCC tissues, and its expression was negatively correlated with the survival of HCC patients. WDR34 promoted the growth, colony formation and migration of HCC cells. However, knocking down the expression of WDR34 inhibited the growth, colony formation and migration of HCC cells.
Taken together, this study demonstrated the oncogenic roles of WDR34 in the progression of HCC and suggested that WDR34 might be a therapeutic target for HCC.
KeywordsHepatocellular carcinoma Beta-catenin/TCF signaling WDR34 Cell growth and migration
Shaochuang Wang designed this project. Xiaoling Luo, Yuting Liu, Shijie Ma, Lei Liu and Rui Xie performed the experiments.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
Human and animal rights
All applicable international, national and/or institutional guidelines for the care and use of animals were followed. All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Declaration of Helsinki and its later amendments or comparable ethical standards.
- 12.Jiao Y, Ban KC, Cao J, Yue HY, Luo Y, Su JJ. Expression and exon 3 mutation of beta-catenin in human hepatocellular carcinoma. Ai Zheng. 2007;26:1085–1089.Google Scholar