NAFLD Induction Delays Postoperative Liver Regeneration of ALPPS in Rats
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Associating liver partition and portal vein ligation (ALPPS) is a promising two-step hepatectomy that is beneficial for accumulative regeneration of the future liver remnant (FLR) and avoids postoperative liver failure.
Our study aimed to evaluate whether nonalcoholic fatty liver disease affected the liver regeneration induced by ALPPS.
Sprague-Dawley rats fed a high-fat diet were used to construct the NAFLD model. ALPPS were performed, and blood and future liver remnant samples were collected at postoperative days 1 (POD1), POD3, and POD7.
The hepatic regeneration rate (HRR) of ALPPS was higher than that of portal vein ligation (PVL) at POD3 and POD7 (p < 0.05), and the number of Ki-67-positive hepatocytes (POD3) and CD68-positive Kupffer cells (POD7) per visual field was higher in the ALPPS group than in the PVL group (p < 0.05). The serum TNF-α, hepatocyte growth factor protein, and the serum IL-6 level were higher in the ALPPS group than in the PVL group at POD3 and POD7. Compared with those of the standard laboratory diet (SLD)-fed rats, the rats with NAFLD exhibited a decrease in the HRR, Ki-67-positive hepatocytes, and CD68-positive Kupffer cells in the FLR. The number of CD68-positive Kupffer cells was lower in rats with NAFLD than that in SLD-fed rats; noteworthily, the serum level of IL-6 and TNF-α changed dramatically after surgeries.
NAFLD induction delayed liver regeneration induced by the ALPPS procedure, which might be associated with hepatocyte proliferation and the number of Kupffer cells.
KeywordsNAFLD ALPPS Liver regeneration Hepatocyte proliferation Kupffer cells
Portal vein ligation
Standard laboratory diet
Hepatocyte growth factor
Left median lobe
Left lateral lobe
Postoperative liver failure
Portal branch embolization
Associating liver partition and portal vein ligation
Nonalcoholic fatty liver disease
This work was supported by the Fund for Science and Technology Development of Jilin Province (20160101060JC, 20150101108JC); the National Key R&D Program of China (2017YFD0502200, 2016YFD0501302); the Project of the Education Department of Jilin Province (No. 2016444).
Compliance with ethical standards
Conflict of interest
All authors declare no conflicts of interest.
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