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Digestive Diseases and Sciences

, Volume 58, Issue 3, pp 706–714 | Cite as

RETRACTED ARTICLE: Effect of miR-451 on the Biological Behavior of the Esophageal Carcinoma Cell Line EC9706

  • Tao Wang
  • Wen-qiao Zang
  • Min Li
  • Na Wang
  • Yu-ling ZhengEmail author
  • Guo-qiang ZhaoEmail author
Original Article

Abstract

Background

MicroRNAs play important roles in coordinating a variety of cellular processes. Abnormal expression of miRNAs has been linked to several cancers. However, the functional role of miR-451 in esophageal squamous cell carcinoma remains unclear.

Aims

The present study explored the effects of miR-451 on the biological behavior of the esophageal carcinoma cell line EC9706.

Methods

Synthetic miR-451 mimics were transfected into EC9706 cells using Lipofectamine™ 2000. The expression of miR-451 was analyzed by RT–PCR and the expressions of Bcl-2, AKT and phosphorylated AKT were analyzed by Western blotting. The MTT assay, soft agar colony formation assay, transwell assay and FACS were used to assess the effect of miR-451 on EC9706 cell proliferation, invasion, metastasis and apoptosis. Tumor growth was assessed by subcutaneous inoculation of cells into BALB/c nude mice.

Results

In comparison to the controls, a significant increase in the expression of miR-451 was associated with significantly decreased expressions of Bcl-2, AKT and p-AKT, and a significant increase in the apoptosis rate. The number of cell clones was significantly decreased by miR-451 expression, which also caused the inhibition of cell proliferation. The average number of cells penetrating the matrigel was significantly lower than the controls. Injection of miR-451 inhibited tumor growth in a xenograft model.

Conclusions

Upregulated expression of miR-451 induced apoptosis and suppressed cell proliferation, invasion and metastasis in the esophageal carcinoma cell line EC9706. In addition, injection of miR-451 inhibited tumor growth in a xenograft model of esophageal cancer.

Keywords

Esophageal neoplasms miR-451 Cell proliferation Apoptosis Neoplasm invasiveness 

Notes

Conflict of interest

None.

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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  1. 1.Department of TumorThe First Affiliated Hospital of Zhengzhou UniversityZhengzhouPeople’s Republic of China
  2. 2.Department of Hemato-TumorThe First Affiliated Hospital of Henan University of TCMZhengzhouPeople’s Republic of China
  3. 3.Department of Microbiology and Immunology, College of Basic Medical SciencesZhengzhou UniversityZhengzhouPeople’s Republic of China

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