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Digestive Diseases and Sciences

, Volume 49, Issue 11–12, pp 1731–1737 | Cite as

Effect of Glucagon-like Peptide-2 (GLP-2) on Diurnal SGLT1 Expression

  • Anthony P. Ramsanahie
  • Urs V. Berger
  • Michael J. Zinner
  • Edward E. Whang
  • David B. RhoadsEmail author
  • Stanley W. Ashley
Article

Abstract

Glucagon-like peptide 2 (GLP-2) is a 33-amino acid gut peptide that leads to villus hyperplasia and altered gene expression.We examined the effect of chronically administered GLP-2 on diurnal gene expression rhythms using the Na+/glucose cotransporter 1 (SGLT1) as the index. Animals were treated with [Gly2]GLP-2 (twice daily; 1μg/g body weight) or vehicle (control) for 10 days. Rats were killed at either 3 hr or 9 hr after light onset (ZT3 and ZT9, respectively), an interval during which SGLT1 expression exhibits a robust induction. SGLT1 mRNA expression was assessed by Northern blotting and in situ hybridization. SGLT1 protein was examined by immunofluorescence and Western blotting. Tissues from GLP-2-treated rats had increased villus height, crypt depth, and proliferation index (P < 0.05). GLP-2 administration did not alter the diurnal increase in mRNA levels of SGLT1, GLUT2, or GLUT5. However, in GLP-2-treated rats, the SGLT1 protein amount increased at both ZT3 and ZT9. Moreover, SGLT1 was preferentially localized to the apical membranes in this group. GLP-2 does not adversely affect the diurnal expression rhythm of SGLT1 and appears to increase membrane expression of the protein. These biological actions of GLP-2 may contribute to its therapeutic value in intestinal diseases.

gastrointestinal hormones monosaccharide transport proteins intestinal epithelium diurnal rhythm gene expression regulation 

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Copyright information

© Springer Science+Business Media, Inc. 2004

Authors and Affiliations

  • Anthony P. Ramsanahie
    • 1
  • Urs V. Berger
    • 2
  • Michael J. Zinner
    • 1
  • Edward E. Whang
    • 1
  • David B. Rhoads
    • 3
    Email author
  • Stanley W. Ashley
    • 1
  1. 1.Department of Surgery, Brigham and Women’s HospitalHarvard Medical SchoolBoston
  2. 2.UB–In SituNatick
  3. 3.Pediatric Endocrine Unit, MassGeneral Hospital for ChildrenHarvard Medical SchoolBostonUSA

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