Sambucus nigra L. ameliorates UVB-induced photoaging and inflammatory response in human skin keratinocytes
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Sambucus nigra L. (Elderberry) is widely used as a dietary supplement in functional food and possesses many pharmacological activities to prevent ailments, such as the colds and fever, diabetes and cancer. However, research on its skin anti-aging effect is still limited. Here, we evaluated the recovery effects of elderberry extract (EB) in UVB-irradiated human skin keratinocytes (HaCaTs) and investigated whether EB represents a potential therapeutic agent against skin photoaging and inflammation. In this study, EB showed good efficiency on scavenging free radicals and dose-dependently reduced reactive oxygen species (ROS) generation. EB notably decreased UVB-induced matrix metalloproteinase-1 (MMP-1) expression and inflammatory cytokine secretion through the inhibition of mitogen-activated protein kinases/activator protein 1 (MAPK/AP-1) and nuclear factor-κB (NF-κB) signaling pathways, blocking extracellular matrix (ECM) degradation and inflammation in UVB-irradiated HaCaTs. In addition, EB improved nuclear factor E2-related factor 2/heme oxygenase-1 (Nrf2/HO-1) signaling to increase oxidative defense capacity, and enhanced transforming growth factor beta (TGF-β) signaling activation to promote procollagen type I synthesis, relieving UVB-induced skin cell damage. These results indicated that EB has the potential to ameliorate UVB-induced skin photoaging and inflammation.
KeywordsElderberry UVB irradiation HaCaTs Photoaging ROS MMP-1
This work was supported by the Snow White Factory Inc., Republic of Korea.
Compliance with ethical standards
Conflicts of interest
The authors declare that they have no conflicts of interest.
- Park B, Hwang E, Seo SA, Zhang MY, Park SY, Yi TH (2017) Dietary Rosa damascena protects against UVB-induced skin aging by improving collagen synthesis via MMPs reduction through alterations of c-Jun and c-Fos and TGF-β1 stimulation mediated smad2/3 and smad7. J Funct Foods 36:480–489CrossRefGoogle Scholar
- Piao MJ, Zhang R, Lee NH, Hyun JW (2012) Phloroglucinol attenuates ultraviolet B radiation-induced matrix metalloproteinase-1 production in human keratinocytes via inhibitory actions against mitogen-activated protein kinases and activator protein-1. Photochem Photobiol 88:381–388CrossRefGoogle Scholar
- Porée B et al (2008) Interleukin-6 (IL-6) and/or soluble IL-6 receptor down-regulation of human type II collagen gene expression in articular chondrocytes requires a decrease of Sp1· Sp3 ratio and of the binding activity of both factors to the COL2A1 promoter. J Biol Chem 283:4850–4865CrossRefGoogle Scholar
- Quan TH, Qin ZP, Xia W, Shao Y, Voorhees JJ, Fisher GJ (2009) Matrix-degrading metalloproteinases in photoaging. In: Journal of investigation dermatology symposium proceedings. pp 20–24Google Scholar