Boron mediated 2D and 3D cultures of adipose derived mesenchymal stem cells
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Boron (B), which is a beneficial bioactive element for human, has an increasing interest in tissue engineering for the last 5 years. However, the effective B concentration in cell culture is still unknown. The aim of the present study is to investigate in vitro osteogenic potential of mesenchymal stem cells, isolated from adipose tissue (AdMSCs), on boron containing 2D and 3D cell cultures. At first, the effects of B concentrations between 1 and 20 μg/mL were evaluated on the survival and osteogenic differentiation of AdMSCs cultured on 2D cell cultures. The 3D cultures were established by using chitosan (Ch) scaffolds prepared by freeze-drying and Ch scaffolds combined with hydroxyapatite (HAp) and B containing hydroxyapatite (B-HAp) that are produced by microwave-induced biomimetic method. The proliferation and osteogenic differentiation of AdMSCs on Ch, HAp/Ch and B-HAp/Ch scaffolds were investigated by in vitro cell culture studies. The results were evaluated with respect to cell viability, bone related ECM gene expressions, and cellular morphology. It was demonstrated that cellular functions of AdMSCs were enhanced by boron in both 2D and 3D cultures. Especially, B-HAp/Ch scaffolds, which have both osteoinductive and osteoconductive properties based on presence of B and HAp in its structure, promoted adhesion, proliferation and osteogenic differentiation of AdMSCs.
KeywordsBoron Chitosan Hydroxyapatite Adipose derived mesenchymal stem cells Bone tissue engineering
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
- Asghari Sana F, Çapkın Yurtsever M, Kaynak Bayrak G, Tunçay EÖ, Kiremitçi AS, Gümüşderelioğlu M (2017) Spreading, proliferation and differentiation of human dental pulp stem cells on chitosan scaffolds immobilized with RGD or fibronectin. Cytotechnology 69:617–630. https://doi.org/10.1007/s10616-017-0072-9 CrossRefGoogle Scholar
- Çapkın M, Çakmak S, Kurt FÖ, Gümüşderelioğlu M, Şen BH, Türk BT, Deliloğlu-Gürhan Sİ (2012) Random/aligned electrospun PCL/PCL-collagen nanofibrous membranes: comparison of neural differentiation of rat AdMSCs and BMSCs. Biomed Mater 7:045013. https://doi.org/10.1088/1748-6041/7/4/045013 CrossRefGoogle Scholar
- Doğan A, Demirci S, Çağlayan AB, Kılıç E, Günal MY, Uslu Ü, Cumbul A, Şahin F (2014) Sodium pentaborate pentahydrate and pluronic containing hydrogel increases cutaneous wound healing in vitro and in vivo. Biol Trace Elem Res 162:72–79. https://doi.org/10.1007/s12011-014-0104-7 CrossRefGoogle Scholar
- Dominici M, Le Blanc K, Mueller I, Slaper-Cortenbach I, Marini F, Krause D, Deans R, Keating A, Dj Prockop, Horwitz E (2006) Minimal criteria for defining multipotent mesenchymal stromal cells. The international society for cellular therapy position statement. Cytotherapy 8:315–317. https://doi.org/10.1080/14653240600855905 CrossRefGoogle Scholar
- Movahedi Najafabadi B, Abnosi MH (2016) Boron induces early matrix mineralization via calcium deposition and elevation of alkaline phosphatase activity in differentiated rat bone marrow mesenchymal stem cells. Cell J 18:62–73Google Scholar
- Rodbell M (1964) The metabolism of isolated fat cells. Compr Physiol 239:375–380Google Scholar
- Wu C, Miron R, Sculean A, Kaskel S, Doert T, Schulze R, Zhang Y (2011) Proliferation, differentiation and gene expression of osteoblasts in boron-containing associated with dexamethasone deliver from mesoporous bioactive glass scaffolds. Biomaterials 32:7068–7078. https://doi.org/10.1016/j.biomaterials.2011.06.009 CrossRefGoogle Scholar