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Cytotechnology

, Volume 71, Issue 2, pp 573–581 | Cite as

Alkali-soluble pectin suppresses IgE production in human myeloma cell line in vitro

  • Akira Iwamoto
  • Yuichi InoueEmail author
  • Hirofumi Tachibana
  • Hiroharu Kawahara
JAACT Issue
  • 43 Downloads

Abstract

We found that strawberry (Fragaria x ananassa) extract has an IgE production suppressive activity and its oral administration improved skin manifestation in atopic dermatitis model mice. In present study, we identified an active substance using the IgE-producing human myeloma cell line U266. Gel filtration experiment indicated that the IgE suppressor was more than 6 kDa in molecular size. In addition, its pectinase treatment inhibited the activity, suggesting that the active substance in strawberry extract is pectin. Among solutions of water-(WP), hexametaphosphate-(HXP), acid-(HP) and alkali soluble pectin (OHP) extracted from strawberry, only OHP suppressed IgE production, and their suppressive activity was cancelled by pectinase treatment. In addition, OHP extracted from apple also inhibited IgE production. Furthermore, OHP also suppressed IgE production and did not affect IgG and IgM production in human peripheral blood mononuclear cells in an in vitro immunization condition. From these results, we concluded that OHP was an IgE suppressor in strawberry extract.

Keywords

IgE Allergy Strawberry Pectin U266 PBMC 

Abbreviations

AIS

Alcohol-insoluble residue

BSA

Bovine serum albumin

ELISA

Enzyme-linked immunosorbent assay

FBS

Fetal bovine serum

FcεRI

High-affinity IgE receptor

GATA3

GATA binding protein 3

HP

Acid-soluble pectin

HRP

Horseradish peroxidase

HXP

Hexametaphosphate-soluble pectin

IFN

Interferon

Ig

Immunoglobulin

IL

Interleukin

IVI

In vitro immunization

MDP

Muramyl dipeptide

OHP

Alkali-soluble pectin

PBMCs

Peripheral blood mononuclear cells

PBS

Phosphate buffered saline

Th

Helper T

WP

Water-soluble pectin

Notes

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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  • Akira Iwamoto
    • 1
  • Yuichi Inoue
    • 2
    Email author
  • Hirofumi Tachibana
    • 1
  • Hiroharu Kawahara
    • 2
  1. 1.Division of Applied Biological Chemistry, Department of Bioscience and Biochemistry, Faculty of AgricultureKyushu UniversityFukuokaJapan
  2. 2.Department of Creative EngineeringNational Institute of Technology, Kitakyushu CollegeKitakyushuJapan

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