, Volume 71, Issue 2, pp 573–581 | Cite as

Alkali-soluble pectin suppresses IgE production in human myeloma cell line in vitro

  • Akira Iwamoto
  • Yuichi InoueEmail author
  • Hirofumi Tachibana
  • Hiroharu Kawahara


We found that strawberry (Fragaria x ananassa) extract has an IgE production suppressive activity and its oral administration improved skin manifestation in atopic dermatitis model mice. In present study, we identified an active substance using the IgE-producing human myeloma cell line U266. Gel filtration experiment indicated that the IgE suppressor was more than 6 kDa in molecular size. In addition, its pectinase treatment inhibited the activity, suggesting that the active substance in strawberry extract is pectin. Among solutions of water-(WP), hexametaphosphate-(HXP), acid-(HP) and alkali soluble pectin (OHP) extracted from strawberry, only OHP suppressed IgE production, and their suppressive activity was cancelled by pectinase treatment. In addition, OHP extracted from apple also inhibited IgE production. Furthermore, OHP also suppressed IgE production and did not affect IgG and IgM production in human peripheral blood mononuclear cells in an in vitro immunization condition. From these results, we concluded that OHP was an IgE suppressor in strawberry extract.


IgE Allergy Strawberry Pectin U266 PBMC 



Alcohol-insoluble residue


Bovine serum albumin


Enzyme-linked immunosorbent assay


Fetal bovine serum


High-affinity IgE receptor


GATA binding protein 3


Acid-soluble pectin


Horseradish peroxidase


Hexametaphosphate-soluble pectin








In vitro immunization


Muramyl dipeptide


Alkali-soluble pectin


Peripheral blood mononuclear cells


Phosphate buffered saline


Helper T


Water-soluble pectin



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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  • Akira Iwamoto
    • 1
  • Yuichi Inoue
    • 2
    Email author
  • Hirofumi Tachibana
    • 1
  • Hiroharu Kawahara
    • 2
  1. 1.Division of Applied Biological Chemistry, Department of Bioscience and Biochemistry, Faculty of AgricultureKyushu UniversityFukuokaJapan
  2. 2.Department of Creative EngineeringNational Institute of Technology, Kitakyushu CollegeKitakyushuJapan

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