, Volume 71, Issue 1, pp 345–350 | Cite as

Cytotoxic and antioxidant properties of essential oil of Centaurea behen L. in vitro

  • Fatih Çağlar ÇelikezenEmail author
  • Şükrü Hayta
  • Özlem Özdemir
  • Hasan Türkez
Original Article


Centaurea species of Asteraceae family are widely use in traditional medicine. Despite wide medicinal use of Centaurea sp., there is limited knowledge concerning Centaurea behen toxicity. Therefore, in this study, it is aimed to determine cytotoxic and oxidative effects of essential oil of C. behen on human blood cell cultures. 3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyltetrazolium bromide (MTT) and lactate dehydrogenase (LDH) release assays were performed to determine cytotoxic effects. In addition, total antioxidant capacity (TAC) and total oxidative status (TOS) were examined to determine oxidative potentials. The results indicated that all tested concentrations of essential oil of C. behen were cytotoxic and led to decreases of cell viability in both assays. Besides, C. behen led to significant increases of TOS levels and decreases of TAC levels. As a conclusion, the present study showed for the first time the cytotoxic and oxidant effects of essential oil of C. behen on cultured human whole blood cells.


Centaurea behen L. Asteraceae Cytotoxicity Oxidative effect Peripheral human blood 


Compliance withe ethical standards

Conflict of interest

No potential conflict of interest was reported by the authors.


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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  • Fatih Çağlar Çelikezen
    • 1
    Email author
  • Şükrü Hayta
    • 2
  • Özlem Özdemir
    • 3
  • Hasan Türkez
    • 3
    • 4
  1. 1.Department of Chemistry, Faculty of Science and LettersBitlis Eren UniversityBitlisTurkey
  2. 2.Department of Environmental EngineeringBitlis Eren UniversityBitlisTurkey
  3. 3.Department of Molecular Biology and GeneticsErzurum Technical UniversityErzurumTurkey
  4. 4.Department of Pharmacy“G. d’Annunzio” University of Chieti-PescaraChieti ScaloItaly

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