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Cytotechnology

, Volume 71, Issue 1, pp 345–350 | Cite as

Cytotoxic and antioxidant properties of essential oil of Centaurea behen L. in vitro

  • Fatih Çağlar ÇelikezenEmail author
  • Şükrü Hayta
  • Özlem Özdemir
  • Hasan Türkez
Original Article
  • 82 Downloads

Abstract

Centaurea species of Asteraceae family are widely use in traditional medicine. Despite wide medicinal use of Centaurea sp., there is limited knowledge concerning Centaurea behen toxicity. Therefore, in this study, it is aimed to determine cytotoxic and oxidative effects of essential oil of C. behen on human blood cell cultures. 3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyltetrazolium bromide (MTT) and lactate dehydrogenase (LDH) release assays were performed to determine cytotoxic effects. In addition, total antioxidant capacity (TAC) and total oxidative status (TOS) were examined to determine oxidative potentials. The results indicated that all tested concentrations of essential oil of C. behen were cytotoxic and led to decreases of cell viability in both assays. Besides, C. behen led to significant increases of TOS levels and decreases of TAC levels. As a conclusion, the present study showed for the first time the cytotoxic and oxidant effects of essential oil of C. behen on cultured human whole blood cells.

Keywords

Centaurea behen L. Asteraceae Cytotoxicity Oxidative effect Peripheral human blood 

Notes

Compliance withe ethical standards

Conflict of interest

No potential conflict of interest was reported by the authors.

References

  1. Al-Easa HS, Rizk AM (1992) Constituents of Centaurea species. Qatar Univ Sci J 12:27–57Google Scholar
  2. Bach SM, Fortuna MA, Attarian R, de Trimarco JT, Catalán CA, Av-Gay Y, Bach H (2011) Antibacterial and cytotoxic activities of the sesquiterpene lactones cnicin and onopordopicrin. Nat Prod Commun 6:163–166Google Scholar
  3. Çelik K, Toğar B, Turkez H, Taşpınar N (2014) In vitro cytotoxic, genotoxic and oxidative effects of acyclic sesquiterpene farnesene. Turk J Biol 38:253–259CrossRefGoogle Scholar
  4. Chen H, Yoshioka H, Kim GS, Jung JE, Okami N, Sakata H, Maier CM, Narasimhan P, Goeders CE, Chan PH (2011) Oxidative stress in ischemic brain damage: mechanisms of cell death and potential molecular targets for neuroprotection. Antioxid Redox Signal 14:1505–1517CrossRefGoogle Scholar
  5. Chougule P, Pawar R, Limaye D, Joshi YM, Kadam V (2012) In-Vitro antioxidant activity of ethanolic extract of Centaurea behen. J Appl Pharm Sci 2:106–110CrossRefGoogle Scholar
  6. Chougule P, Yadunath J, Ashish J (2014) Protective effect of ethanol extract of Centaurea behen Linn in. carbon tetra chloride-induced hepatitis in rats. Int J Pharm Pharm Sci 6:197–200Google Scholar
  7. Conforti F, Menichini F, Loizzo MR, Statti AG, Rapisarda A, Menichini F, Houghton PJ (2008) Antioxidant, a-amylase inhibitory and brine-shrimp toxicity studies on Centaurea centaurium L. methanolic root extract. Nat Prod Res 22:1457–1466CrossRefGoogle Scholar
  8. Csupor-Loffler B, Hajdu Z, Rethy B, Zupko I, Mathe I, Redei T, Falkay G, Hohmann J (2009) Antiproliferative activity of Hungarian asteraceae species against human cancer cell lines. Part II. Phytother Res 23:1109–1115CrossRefGoogle Scholar
  9. Davis PH, Mill RR, Tan K (1988) Flora of Turkey and the east aegean islands. Edinburgh University Press, EdinburgGoogle Scholar
  10. Duan X, Wen Z, Shen H, Shen M, Chen G (2016) Intracerebral hemorrhage, oxidative stress, and antioxidant therapy. Oxid Med Cell Longev.  https://doi.org/10.1155/2016/1203285 Google Scholar
  11. Erel SB, Karaalp C, Bedir E, Kaehlig H, Glasl S, Khan S, Krenn L (2011) Secondary metabolites of Centaurea calolepis and evaluation of cnicin for anti-inflammatory, antioxidant, and cytotoxic activities. Pharm Biol 49:840–849CrossRefGoogle Scholar
  12. Erel SB, Demir S, Nalbantsoy A, Ballar P, Khan S, Karabay Yavasoglu NU, Karaalp C (2014) Bioactivity screening of five Centaurea species and in vivo anti-inflammatory activity of C. athoa. Pharm Biol 52:775–781CrossRefGoogle Scholar
  13. Erol-Dayi Ö, Pekmez M, Bona M, Aras-Perk A, Arda N (2011) Total phenolic contents, antioxidant activities and cytotoxicity of three Centaurea species: C. calcitrapa subsp. calcitrapa, C. Ptosimopappa and C. spicata. Free Radic Antioxid 1:31–36CrossRefGoogle Scholar
  14. Escher GB, Santos JS, Rosso ND, Marques MB, Azevedo L, Vieira do Carmo AM, Daguer H, Molognoni L, Do Prado-Silva L, Sant’Ana AS, Da Silva MC, Granato D (2018) Chemical study, antioxidant, anti-hypertensive, and cytotoxic/cytoprotective activities of Centeurea cyanus L. petals aqueous extract. Food Chem Toxicol 118:439–453CrossRefGoogle Scholar
  15. Esmaeili A, Khodadadi E (2012) Volatile compounds of essential oil Centaurea behen L. grown in Iran. J Paramed Sci 3:8–11Google Scholar
  16. Evans HJ, O’Riordan ML (1975) Human peripheral blood lymphocytes for the analysis of chromosome aberrations in mutagen tests. Mutat Res 31:135–148CrossRefGoogle Scholar
  17. Flamini G, Stoppelli G, Morelli I, Ertugrul K, Dural H, Tugay O, Demirelma H (2004) Secondary metabolites from Centaurea isaurica from Turkey and their chemotaxonomical significance. Biochem Systemat Ecol 32:553–557CrossRefGoogle Scholar
  18. Funk VA, Bayer RJ, Keeley S, Chan R, Watson L, Gemeinholzer B, Schilling E, Panero JL, Baldwin BG, Garcia- Jacas N, Susanna A, Jansen RK (2005) Everywhere but Antarctica: using a super tree to understand the diversity and distribution of the Compositae. Biol Skr 55:343–374Google Scholar
  19. Garcia-Jacas N, Susanna A, Mozaffarian V, Ilarslan R (2000) The natural delimitation of Centaurea (Asteraceae: Cardueae): ITS sequence analysis of the Centaurea jacea group. Plant Systemat Evol 223:185–199CrossRefGoogle Scholar
  20. Geyikoğlu F, Turkez H (2006) Protective effect of sodium selenite against the genotoxicity of aflatoxin B-1 in human whole blood cultures. Braz Arch Biol Technol 49:393–398CrossRefGoogle Scholar
  21. Geyikoğlu F, Turkez H, Keles MS (2005) The role of fruit juices in the prevention of aluminum sulphate toxicity in human blood in vitro. Fresenius Environ Bull 14:878–883Google Scholar
  22. Gonzalez AG, Barrera JB, Garcia TZ, Rosas FE (1984) Sesquiterpene lactones from Centaurea species. Phytochemistry 23:2071–2072CrossRefGoogle Scholar
  23. Granato D, Calado VMA, Jarvis B (2014) Observations on the use of statistical methods in food science and technology. Food Res Int 55:137–149CrossRefGoogle Scholar
  24. Hussain SM, Hess KL, Gearhart JM, Geiss KT, Schlager JJ (2005) In vitro toxicity of nanoparticles in BRL 3A rat liver cells. Toxicol In Vitro 19:975–983CrossRefGoogle Scholar
  25. Karamenderes C, Khan S, Tekwani BL, Jacob MR, Khan IA (2006) Antiprotozoal and antimicrobial activities of Centaurea species growing in Turkey. Pharm Biol 44:534–539CrossRefGoogle Scholar
  26. Kaskoos RA (2013) In-vitro a-glucosidase inhibition and antioxidant activity of methanolic extract of Centaurea calcitrapa from Iraq. Am J Essent Oils Nat Prod 1:122–125Google Scholar
  27. Kilic O (2013) Essential oil compounds of three Centaurea L. taxa from Turkey and their chemotaxonomy. J Med Plants Res 7:1344–1350Google Scholar
  28. Kubacey TM, Haggag EG, El-Toumy Sayed AA, El-A Amany A, Ibrahim M, Youns MM (2012) Biological activity and flavonoids from Centaurea alexanderina leaf extract. J Pharm Res 5:3352–3361Google Scholar
  29. Kurian A, Sankar A (2007) Medicinal plants. Horticulture Science Series. New India Publishing, New DelhiGoogle Scholar
  30. Lau A, Wang Y, Chiu JF (2008) Reactive oxygen species: current knowledge and applications in cancer research and therapeutic. J Cell Biochem 104:657–667.  https://doi.org/10.1002/(ISSN)1097-4644 CrossRefGoogle Scholar
  31. Lewerenz V, Hanelt S, Nastevska C, El-Bahay C, Rouhrdanz E, Kahl R (2003) Antioxidants protect primary rat hepatocyte cultures against acetaminophen-induced DNA strand breaks but not against acetaminophen-induced cytotoxicity. Toxicology 191:179–187CrossRefGoogle Scholar
  32. López-Rodriguez M, Garcia VP, Zater H, Benayache S, Benayache F (2009) Cynaratriol, a sesquiterpene lactones from Centaurea musimomum. Acta Cryst E65:867–868Google Scholar
  33. Medjroubi K, Benayache F, Bermejo J (2005) Sesquiterpene lactones from Centaurea musimomum. Antiplasmodial and cytotoxic activities. Fitoterapia 76:744–745CrossRefGoogle Scholar
  34. Mishio T, Honma T, Iwasshina T (2006) Yellow flavonoid in Centaurea ruthenica as flower pigments. Biochem Systemat Ecol 34:180–184CrossRefGoogle Scholar
  35. Newman DJ, Cragg GM (2016) Natural products as sources of new drugs from 1981 to 2014. J Nat Prod 79:629–661CrossRefGoogle Scholar
  36. Pires Tania CSP, Dias MI, Barrosa L, Calhelhaa RC, Alves MJ, Oliveira M, Beatriz PP, Buelga CS, Ferreira Isabel CFR (2018) Edible flowers as sources of phenolic compounds with bioactive potential. Food Res Int 105:580–588CrossRefGoogle Scholar
  37. Sarker SD, Savchenko T, Whiting P, Sik V, Dinan LN (1997) Moschamine, cis-moschamine, moschamine, moschamindole and moschamindolol: four novel indole alkaloids from Centaurea moschata. Nat Prod Lett 9:189–199CrossRefGoogle Scholar
  38. Seghiri R, Boumaza O, Mekkiou R, Benayache S, Mosset P, Quintana J, Estevez F, Leon F, Bermejo J, Benayache F (2009) A flavonoid with cytotoxic activity and other constituents from Centaurea africana. Phytochem Lett 2:114–118CrossRefGoogle Scholar
  39. Türkez H, Şişman T (2007) Anti-genotoxic effect of hydrated sodium calcium aluminosilicate on genotoxicity to human lymphocytes induced by aflatoxin b1. Toxicol Ind Health 23:83–89.  https://doi.org/10.1177/0748233707076738 CrossRefGoogle Scholar
  40. Ugur A, Duru ME, Ceylan O, Sarac N, Varol O, Kivrak I (2009a) Chemical composition, antimicrobial and antioxidant activities of Centaurea ensiformis Hub.-Mor. (Asteraceae), a species endemic to Muğla (Turkey). Nat Prod Res 23:149–167CrossRefGoogle Scholar
  41. Ugur A, Sarac N, Ceylan O, Duru ME (2009b) Chemical composition of endemic Centaurea austro-anatolica and studies of its antimicrobial activity againstmulti-resistant bacteria. Acta Pharm 59:463–472CrossRefGoogle Scholar
  42. Ugur A, Sarac N, Ceylan O, Duru ME (2010) Antimicrobial activity and chemical composition of endemic Centaurea cariensis subsp. niveo-tomentosa. Nat Prod Res 24:861–872CrossRefGoogle Scholar
  43. Uzunhisarcıklı ME, Teksen M, Dogan E (2005) Centaurea marashica (Asteraceae) a new species from Turkey. Ann Bot Fenn 42:309–312Google Scholar
  44. Wang T, Jiang A, Zhang J, Jing F (2010) Apoptosis induction by E2F decoy DNA of the prostate cancer cell line. Braz Arch Biol Technol 53:327–334CrossRefGoogle Scholar
  45. Yeum K-J, Russell MR, Krinsky IN, Adlini G (2004) Biomarkers of antioxidant capacity in hydrophilic and lipophilic compartments of human plasma. Arch Biochem Biophys 430:97–103CrossRefGoogle Scholar

Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  • Fatih Çağlar Çelikezen
    • 1
    Email author
  • Şükrü Hayta
    • 2
  • Özlem Özdemir
    • 3
  • Hasan Türkez
    • 3
    • 4
  1. 1.Department of Chemistry, Faculty of Science and LettersBitlis Eren UniversityBitlisTurkey
  2. 2.Department of Environmental EngineeringBitlis Eren UniversityBitlisTurkey
  3. 3.Department of Molecular Biology and GeneticsErzurum Technical UniversityErzurumTurkey
  4. 4.Department of Pharmacy“G. d’Annunzio” University of Chieti-PescaraChieti ScaloItaly

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