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Isolation and characterization of ovine umbilical cord-derived mesenchymal stem cells

  • Sirguleng Zhao
  • Li Tao
  • Yunyun Tian
  • Dapeng Tai
  • Pengxia LiuEmail author
  • Dongjun LiuEmail author
Original Article

Abstract

Mesenchymal stem cells (MSCs) are able to self-renew and have multi-lineage differentiation potential. However, studies on ovine umbilical cord-derived MSCs (UC-MSCs) are limited. Our study aimed to isolate and characterize ovine UC-MSCs. We successfully isolated ovine UC-MSCs and defined their surface marker profile using immunofluorescence analysis. Ovine UC-MSCs were found to be positive for cell surface markers CD13, CD29, CD44, CD90, and CD106, and negative for cell surface marker CD45. Assessment of the proliferation potential of ovine UC-MSCs showed that from day 3 of cultivation a plateau phase was reached. And compare to passage 10, 15, 20 cells, passage 5 cells proliferating the fastest. Differentiation of ovine UC-MSCs into adipocytes, osteocytes, and chondrocytes was also demonstrated by staining for tissue-specific markers and using quantitative real-time polymerase chain reaction for specific marker gene expression. This study demonstrates the existence of a MSC population within the ovine umbilical cord, which maintained a normal karyotype up to passage 20.

Keywords

Isolation Characterization Mesenchymal stem cells Ovine Umbilical cord 

Notes

Acknowledgements

This work was supported by the National Natural Science Foundation of China (No. 31101698) and the Inner Mongolia Natural Science Foundation for Distinguished Young Scholars Development (No. 2011JQ03). We are grateful to all who contributed to this work at the Department of Biology of Inner Mongolia University.

Author’s contributions

The experiment was designed by PL and DL, performed and analyzed by SZ, LT, YT, and DT. The manuscript was written by SZ and PL.

Complain with ethical standards

Conflict of interest

The authors declare that they have no competing interests.

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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  1. 1.State Key Laboratory of Reproductive Regulation & Breeding of Grassland Livestock, School of Life SciencesInner Mongolia UniversityHohhotP.R. China

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