Aldehyde dehydrogenase activity of Wharton jelly mesenchymal stromal cells: isolation and characterization
Mesenchymal stromal cells (MSCs) are promising tools in regenerative medicine and targeted therapies. Although different origins have been described, there is still huge need to find a valuable source harboring specific subpopulations of MSCs with precise therapeutic functions. Here, we isolated by fluorescence activated cell sorting technique, two populations of Wharton’s jelly (WJ)-MSCs based on their aldehyde dehydrogenase (ALDH) activity. Two different ALDH activities (low vs. high) were thus observed. We then analyzed their gene expression profile for stemness, phenotype, response to hypoxia, angiogenesis, hematopoietic support, immunomodulation and multilineage differentiation abilities (osteogenesis, adipogenesis, and chondrogenesis). According to ALDH activity, many differences in the mRNA expression of these populations were noticed. In conclusion, we provide evidences that WJ harbors two distinct populations of MSCs with different ALDH activity. These populations seem to display specific functional competences that may be interesting for concise therapeutic applications.
KeywordsWharton jelly mesenchymal stromal cells Aldehyde dehydrogenase activity Fluorescence activated cell sorting Transcriptome analysis
We thank “Télévie” (FNRS) for the financial support.
Conceived and designed the experiments: MN, LL. Performed the experiments: MN, LL. Analyzed the data: EC, MN, LD. Contributed reagents/materials/analysis tools: LD, LVG. Wrote the paper: EC, MN, LL.
Mehdi Najar is awardee of a “Télévie” post-doctoral fellowship and Emerence Crompot is awardee of Ph.D. Grant “Télévie” (F.N.R.S).
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
This study was approved by the Bordet Institute Ethics Committee (Belgium) and conducted in accordance with the Declaration of Helsinki (1964). All donors and/or their parents gave written informed consent.
Availability of data and materials
All data generated or analyzed during this study are included in this published article (and its supplementary information files).
- Atouf F, Provost NM, Rosenthal FM (2013) Standards for ancillary materials used in cell- and tissue-based therapies. MBA Sunday, September 1, 2013. http://www.bioprocessintl.com/upstream-processing/biochemicals-raw-materials/standards-for-ancillary-materials-used-in-cell-and-tissue-based-therapies-346300/
- Batsali AK, Pontikoglou C, Koutroulakis D et al (2017) Differential expression of cell cycle and WNT pathway-related genes accounts for differences in the growth and differentiation potential of Wharton’s jelly and bone marrow-derived mesenchymal stem cells. Stem Cell Res Ther 8:102PubMedPubMedCentralCrossRefGoogle Scholar
- Briquet A, Dubois S, Bekaert S et al (2010) Prolonged ex vivo culture of human bone marrow mesenchymal stem cells influences their supportive activity toward NOD/SCID-repopulating cells and committed progenitor cells of B lymphoid and myeloid lineages. Haematologica 95:47–56PubMedCrossRefPubMedCentralGoogle Scholar
- Fayyad-Kazan M, Najar M, Fayyad-Kazan H, Raicevic G, Lagneaux L (2017) Identification and evaluation of new immunoregulatory genes in mesenchymal stromal cells of different origins: comparison of normal and inflammatory conditions. Med Sci Monit Basic Res 23:87–96PubMedPubMedCentralCrossRefGoogle Scholar
- Mandelli F, Lauria F, Majolino I (1999) Autologous transplantation with peripheral blood stem cells in chronic lymphocytic leukemia. Hematol Cell Ther 41:117–125Google Scholar
- Moreb JS, Ucar D, Han S et al (2012) The enzymatic activity of human aldehyde dehydrogenases 1A2 and 2 (ALDH1A2 and ALDH2) is detected by Aldefluor, inhibited by diethylaminobenzaldehyde and has significant effects on cell proliferation and drug resistance. Chem Biol Interact 195:52–60PubMedCrossRefPubMedCentralGoogle Scholar
- Reppel L, Schiavi J, Charif N et al (2015) Chondrogenic induction of mesenchymal stromal/stem cells from Wharton’s jelly embedded in alginate hydrogel and without added growth factor: an alternative stem cell source for cartilage tissue engineering. Stem Cell Res Ther 6:260PubMedPubMedCentralCrossRefGoogle Scholar
- Stanko P, Kaiserova K, Altanerova V, Altaner C (2014) Comparison of human mesenchymal stem cells derived from dental pulp, bone marrow, adipose tissue, and umbilical cord tissue by gene expression. Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub 158:373–377PubMedCrossRefPubMedCentralGoogle Scholar
- Su Y, Shen X, Chen J et al (2017) Differentially expressed genes in PPARgamma-deficient MSCs. Mol Cell EndocrinolGoogle Scholar