Expanding knowledge on Russula alatoreticula, a novel mushroom from tribal cuisine, with chemical and pharmaceutical relevance
Since antiquity, numerous macrofungi are being worshiped as food and natural medicine especially in Asian tribal communities. Recent investigation has correlated these medicinal properties with bioactive components including phenols and flavonoids. However, research on mushrooms is not satisfactory; as several traditionally prized members remain undiscovered or poorly explored yet. This backdrop tempted us to unveil secondary metabolites empowered with therapeutics from an ethnic delicacy, Russula alatoreticula that was justified as a novel macrofungus in our previous publication. Accordingly, methanol extract was prepared from dried basidiocarps that was found to be enriched with phenolic compounds (pyrogallol > cinnamic acid > p-coumaric acid) and ascorbic acid. As a result, the fraction exhibited strong antioxidant activity evident by the ability of quenching free radicals, chelating Fe2+ ion and reducing components with EC50 of 263–2382 µg/ml. Besides, effective antibacterial potential against six investigated microbes was also noticed where MIC value ranged from 99 to 2673.74 µg/ml. Furthermore, the extract revealed promising anticancer property as it induced apoptosis of Hep3B cell (IC50 358.57 µg/ml) by imparting morphological changes, interfering cell cycle, depleting MMP and alleviating ROS through Bax, Bcl2, caspases 9 and 3 intrinsic mitochondrial pathway. Overall study implied that the immense bioactive potential of R. alatoreticula could possibly be utilized as a good source of natural supplement to combat against free radicals, pathogenic bacteria and hepatocellular carcinoma as well as in food safety industry.
KeywordsAntibacterial potential HPLC Hep3B liver cancer Novel species Phenolics Antioxidant activity
Authors would like to acknowledge the facilities provided by Department of Botany (UGC-CAS Phase VI, VII), University of Calcutta and DST-FIST for instrumental support.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interests.
- Acharya K, Bera I, Khatua S, Rai M (2015a) Pharmacognostic standardization of Grifola frondosa: a well-studied medicinal mushroom. Pharm Lett 7(7):72–78Google Scholar
- Acharya K, Khatua S, Ray S (2017b) Quality assessment and antioxidant study of Pleurotus djamor (Rumph. ex Fr.) Boedijn. J Appl Pharm Sci 7(6):105–110Google Scholar
- Biswal D, Pramanik NR, Chakrabarti S, Drew MGB, Acharya K, Chandra S (2017) Syntheses, crystal structures, DFT calculations, protein interaction and anticancer activities of water soluble dipicolinic acid-imidazole based oxidovanadium(IV) complexes. Dalton Trans 46:16682–16702. https://doi.org/10.1039/C7DT02903A CrossRefPubMedGoogle Scholar
- Boeing JS, Barizão ÉO, Silva BC, Montanher PF, Almeida VC, Visentainer JV (2014) Evaluation of solvent effect on the extraction of phenolic compounds and antioxidant capacities from the berries: application of principal component analysis. Chem Cent J 8:48. https://doi.org/10.1186/s13065-014-0048-1 CrossRefPubMedPubMedCentralGoogle Scholar
- Chatterjee S, Chatterjee A, Chandra S, Khatua S, Saha GK, Acharya K (2016) Tricholoma giganteum ameliorates benzo[α]pyrene-induced lung cancer in mice. Int J Curr Pharm Sci Rev Res 7(5):283–290Google Scholar
- Gursoy N, Sarikurkeu C, Tepe B, Solak MH (2010) Evaluation of antioxidant activities of 3 edible mushrooms: Ramaria flava (Schaef.: Fr.) Quél., Rhizopogon roseolus (Corda) T.M. Fries., and Russula delica Fr. Food Sci Biotechnol 19(3):691–696. https://doi.org/10.1007/s10068-010-0097-8 CrossRefGoogle Scholar
- Khatua S, Paul S, Acharya K (2013) Mushroom as the potential source of new generation of antioxidant: a review. Res J Pharm Technol 6(5):496–505Google Scholar
- Khatua S, Ghosh S, Acharya K (2017b) A simplified method for microtiter based analysis of in vitro antioxidant activity. Asian J Pharm 11(2):S327–S335Google Scholar
- Khatua S, Ghosh S, Acharya K (2017c) Chemical composition and biological activities of methanol extract from Macrocybe lobayensis. J Appl Pharm Sci 7(10):144–151Google Scholar
- Kosanic M, Rankovic B, Dasic M (2013) Antioxidant and antimicrobial properties of mushrooms. Bulg J Agric Sci 19(5):1040–1046Google Scholar
- Kouassi KA, Kouadio EJP, Djè KM, Dué AE, Kouamé LP (2016) Edible ectomycorrhizal mushrooms Russula spp. of Côte d’Ivoire: total phenolic content, HPLC-profiles of phenolic compounds and organic acids, antioxidant activities. J Agric Chem Environ 5:73–84. https://doi.org/10.4236/jacen.2016.52008 CrossRefGoogle Scholar
- Panda MK, Tayung K (2015) Documentation and ethnomedicinal knowledge on wild edible mushrooms among ethnic tribes of northern Odisha, India. Asian J Pharm Clin Res 8(4):139–143Google Scholar
- Ruan-Soto F, Ordaz-Velázquez M, García-Santiago W, Pérez-Ovando EC (2017) Traditional processing and preservation of wild edible mushrooms in Mexico. Ann Food Process Preserv 2(1):1013Google Scholar