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Tenocyte-derived exosomes induce the tenogenic differentiation of mesenchymal stem cells through TGF-β

  • Tianpeng Xu
  • Menglei Xu
  • Jiaxiang Bai
  • Jiayi Lin
  • Binqing Yu
  • Yu Liu
  • Xiaobin Guo
  • Jining Shen
  • Houyi Sun
  • Yuefeng HaoEmail author
  • Dechun GengEmail author
Original Article
  • 39 Downloads

Abstract

Mesenchymal stem cells (MSCs) hold great potential to treat tissue damage based on their multipotent property, and are also considered as suitable cell resources to create tissue-engineered grafts for tendon repair. However, the clinical application of MSCs is still limited by the lack of efficient methods to induce tenogenic differentiation. In this study, by performing the experiments in transwell system, we found that paracrine factors from tenocytes could induce MSCs to undergo the tenogenic differentiation. We further verified that tenocytes could secrete exosomes and these tenocyte-derived exosomes efficiently initiated the tenogenic differentiation of MSCs. Finally, we revealed that the TGF-β existing in tenocyte-derived exosomes mediated the process, as the inhibition of TGF-β signaling abolished the effects of tenocyte-derived exosomes on MSCs. By investigating the effects of tenocytes on MSCs, we found that tenocytes-derived exosomes can induce tenogenic differentiation of MSCs in a TGF-β dependent manner. These studies provided critical information about the multipotency of MSCs and suggested potential strategies for clinical translation.

Keywords

Mesenchymal stem cells Tenocytes Exosomes Tenogenic differentiation 

Notes

Acknowledgements

This research was supported by the National Nature Science Foundation of China (81101399 and 81272018).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  • Tianpeng Xu
    • 1
  • Menglei Xu
    • 1
  • Jiaxiang Bai
    • 2
  • Jiayi Lin
    • 2
  • Binqing Yu
    • 2
  • Yu Liu
    • 2
  • Xiaobin Guo
    • 2
  • Jining Shen
    • 2
  • Houyi Sun
    • 2
  • Yuefeng Hao
    • 1
    Email author
  • Dechun Geng
    • 2
    Email author
  1. 1.Orthopedics and Sports Medicine Center, Suzhou Municipal Hospital (North District)Nanjing Medical University Affiliated Suzhou HospitalSuzhouPeople’s Republic of China
  2. 2.Department of OrthopedicsThe First Affiliated Hospital of Soochow UniversitySuzhouPeople’s Republic of China

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