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Cytotechnology

, Volume 71, Issue 1, pp 1–14 | Cite as

Bone marrow derived endothelial progenitor cells retain their phenotype and functions after a limited number of culture passages and cryopreservation

  • Xianghui GongEmail author
  • Bin Li
  • Yongxing Yang
  • Yan Huang
  • Yan Sun
  • Meili Liu
  • Xiaoling Jia
  • Yubo FanEmail author
Article
  • 116 Downloads

Abstract

A critical limitation for tissue engineering and autologous therapeutic applications of bone marrow derived EPCs is their low frequency, which is even lower in number and activity level in patients with cardiovascular risk factors and other diseases. New strategies for obtaining and reserving sufficient ready-to-use EPCs for clinical use have hit major obstacles, because effects of serial passage and cryopreservation on EPC phenotype and functions are still needed to be explored. The present study aims at investigating effects of a limited number of culture passages as well as cryopreservation on EPC phenotype and functions. We isolated EPCs from rat bone marrow and cultured them up to passage 12 (totaling achievements of 40 population doublings). The phenotype and functions of fresh cultured and post-cryopreserved EPCs at passages 7 and 12, respectively, were evaluated. EPCs at passage 12 maintained the morphological characteristics, marker phenotype, Dil-ac-LDL uptake and FITC-UEA-1 binding functions, enhanced EPCs proliferation, tube formation and migration, but decreased CD133 expression compared with EPCs at passage 7. Cryopreservation caused limited impairment in EPC phenotype and functions. In brief, our results demonstrated that a limited number of culture passages and cryopreservation did not change EPC phenotype and functions, and can be used for the development of robust strategies and quality control criterion for obtaining sufficient and high-quality ready-to-use EPCs for tissue engineering and therapeutic applications.

Keywords

Endothelial progenitor cells Passage Cryopreservation Bone marrow 

Notes

Acknowledgements

This work was supported by the National Natural Science Foundation of China (Nos. 31771019, 11172031, 31470901). The national key research and development plan (No. 2016YFC1101101). International Joint Research Center of Aerospace Biotechnology and 344 Medical Engineering from Ministry of Science and Technology of China, 111 Project 345 (No. B13003).

Compliance with ethical standards

Conflict of interest

The authors have no additional conflict of interest to disclose.

Supplementary material

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Copyright information

© Springer Nature B.V. 2018

Authors and Affiliations

  1. 1.Key Laboratory for Biomechanics and Mechanobiology of Ministry of Education, School of Biological Science and Medical EngineeringBeihang UniversityBeijingPeople’s Republic of China
  2. 2.Beijing Advanced Innovation Centre for Biomedical EngineeringBeihang UniversityBeijingPeople’s Republic of China
  3. 3.National Research Center for Rehabilitation Technical AidsBeijingPeople’s Republic of China

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