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Cytotechnology

, Volume 70, Issue 6, pp 1487–1498 | Cite as

Enhancement of osteogenic differentiation of adipose-derived stem cells by PRP modified nanofibrous scaffold

  • Mandana Kazem-Arki
  • Mahboubeh Kabiri
  • Iman Rad
  • Nasim Hayati Roodbari
  • Hoorieh Hosseinpoor
  • Samaneh Mirzaei
  • Kazem Parivar
  • Hana Hanaee-Ahvaz
Original Article
  • 96 Downloads

Abstract

Recent developments in bone tissue engineering have paved the way for more efficient and cost-effective strategies. Additionally, utilization of autologous sources has been considered very desirable and is increasingly growing. Recently, activated platelet rich plasma (PRP) has been widely used in the field of bone tissue engineering, since it harbours a huge number of growth factors that can enhance osteogenesis and bone regeneration. In the present study, the osteogenic effects of PRP coated nanofibrous PES/PVA scaffolds on adipose-derived mesenchymal stem cells have been investigated. Common osteogenic markers were assayed by real time PCR. Alkaline phosphate activity, calcium deposition and Alizarin red staining assays were performed as well. The results revealed that the highest osteogenic differentiation occurred when cells were cultured on PRP coated PES/PVA scaffolds. Interestingly, direct application of PRP to culture media had no additive effects on osteogenesis of cells cultured on PRP coated PES/PVA scaffolds or those receiving typical osteogenic factors. The highest osteogenic effects were achieved by the simplest and most cost-effective method, i.e. merely by using PRP coated scaffolds. PRP coated PES/PVA scaffolds can maximally induce osteogenesis with no need for extrinsic factors. The major contribution of this paper to the current researches on bone regeneration is to suggest an easy, cost-effective approach to enhance osteogenesis via PRP coated scaffolds, with no additional external growth factors.

Keywords

Bone tissue engineering Adipose-derived mesenchymal stem cell PRP Nanofibrous scaffold 

Notes

Acknowledgements

This work was supported financially by Stem Cell Technology Research Center.

Compliance with ethical standards

Conflict of interest

The authors declare no conflict of interest.

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Copyright information

© Springer Science+Business Media B.V., part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of Biology, Science and Research BranchIslamic Azad UniversityTehranIran
  2. 2.Department of Biotechnology, College of ScienceUniversity of TehranTehranIran
  3. 3.Stem Cell Technology Research CenterTehranIran

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