Protective skin aging effects of cherry blossom extract (Prunus Yedoensis) on oxidative stress and apoptosis in UVB-irradiated HaCaT cells

  • Yaning Wang
  • Weixuan LiEmail author
  • Sika Xu
  • Rong Hu
  • Qingting Zeng
  • Qiaoyuan Liu
  • Shan Li
  • Hayeon Lee
  • Moonsik Chang
  • Lei GuanEmail author


Extracts of the cherry blossom plant have been reported to exert various biological effects on human cells. However, no previous investigations have examined the antioxidant and anti-apoptotic effects of these extracts on ultraviolet B (UVB) radiation-induced skin aging. This study explores the underlying mechanisms of the antioxidant and anti-apoptotic effects of cherry blossom extract (CBE) in human keratinocyte (HaCaT) cells. HaCaT cells were treated with CBE at concentrations of 0.5, 1.0, and 2.0% for 24 h and then irradiated with UVB (40 mJ/cm2). CBE effectively and dose-dependently decreased the levels of reactive oxygen species and malondialdehyde, while increasing the activities of superoxide dismutase and glutathione peroxidase. Pretreatment with 1 and 2% CBE attenuated UVB-induced DNA damage by reducing the formation of cyclobutane pyrimidine dimers and 8-hydroxy-20-deoxyguanosine. Furthermore, CBE also prevented UVB-induced apoptosis and significantly downregulated B cell lymphoma 2 (Bcl-2)-associated X, cytochrome-c, and caspase-3 expression, while upregulating Bcl-2 expression. Taken together, these results indicate that CBE protects HaCaT cells from UVB-induced oxidative stress and apoptosis and suggest that CBE could be a potent antioxidant against skin aging.


Skin aging Cherry blossom extract Antioxidant Anti-apoptosis HaCaT cells 



We are grateful to The Garden of Natural Solution Co., Ltd. for supporting this work.

Compliance with ethical standards

Conflict of interests

The authors have declared that no competing interests exist.

Supplementary material

10616_2018_215_MOESM1_ESM.docx (331 kb)
Supplementary material 1 (DOCX 330 kb)


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Copyright information

© Springer Science+Business Media B.V., part of Springer Nature 2019

Authors and Affiliations

  1. 1.Skin Research CenterLandproof Testing Technology Co., LtdGuangzhouChina
  2. 2.Clinical LaboratoryThe First People Hospital of FoshanFoshanPeople’s Republic of China
  3. 3.The Garden of NaturalsolutionGyeonggi-DoRepublic of Korea

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