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Enhancement of monoclonal antibody production in CHO cells by exposure to He–Ne laser radiation

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Abstract

This study tested the effectiveness of laser biostimulation in small-scale cultures in vitro. We investigated the response of recombinant CHO cells, which are used for the production of monoclonal antibody, to low level laser radiation. The cells were irradiated using a 632.8 nm He–Ne laser in a continuous wave mode at different energy doses. We incubated the irradiated cells in small batch cultures and assessed their proliferation and productivity at various time intervals. Compared to untreated cells, the irradiated cells showed a significant increase in antibody production. Moreover, the results showed that laser irradiation did not affect viability and slightly enhanced proliferation rate.

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Acknowledgments

This project was partially sponsored by the Ministry of Higher Education and Scientific Research, Iraq.

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Authors and Affiliations

  1. School of Chemical and Bioprocess Engineering, Conway Institute for Biomolecular and Biomedical Research, University College Dublin, Belfield, Dublin, Ireland

    Rana Ghaleb, Mariam Naciri, Rasoul Al-Majmaie & Mohamed Al-Rubeai

  2. Institute of Laser for Postgraduate Studies, University of Baghdad, Baghdad, Iraq

    Rana Ghaleb & Amel Maki

Authors
  1. Rana Ghaleb
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  2. Mariam Naciri
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  3. Rasoul Al-Majmaie
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  4. Amel Maki
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  5. Mohamed Al-Rubeai
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Correspondence to Mohamed Al-Rubeai.

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Ghaleb, R., Naciri, M., Al-Majmaie, R. et al. Enhancement of monoclonal antibody production in CHO cells by exposure to He–Ne laser radiation. Cytotechnology 66, 761–767 (2014). https://doi.org/10.1007/s10616-013-9625-8

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  • DOI: https://doi.org/10.1007/s10616-013-9625-8

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