Cytotechnology

, Volume 51, Issue 2, pp 45–50 | Cite as

Identity tests: determination of cell line cross-contamination

  • C. M. Cabrera
  • F. Cobo
  • A. Nieto
  • J. L. Cortés
  • R. M. Montes
  • P. Catalina
  • A. Concha
Review

Abstract

Cell line cross-contamination is a phenomenon that arises as a result of the continuous cell line culture. It has been estimated that around 20% of the cell lines are misidentified, therefore it is necessary to carry out quality control tests for the detection of this issue. Since cell line cross-contamination discovery, different methods have been applied, such as isoenzyme analysis for inter-species cross-contamination; HLA typing, and DNA fingerprinting using short tandem repeat and a variable number of tandem repeat for intra-species cross-contamination. The cell banks in this sense represent the organizations responsible for guaranteeing the authenticity of cell lines for future research and clinical uses.

Keywords

Cell lines Cross-contamination Fingerprinting Isoenzyme analysis Short tandem repeat Variable number tandem repeat 

Abbreviations

AFLP

Amplified fragment length polymorphism

MCB

Master cell bank

STR

Short tandem repeat

SBT

Sequence based typing

SSO

Sequence specific oligonucleotide

SSOP

Sequence specific oligonucleotide probe

VNTR

Variable number tandem repeat

WCB

Working cell bank

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Copyright information

© Springer Science+Business Media B.V. 2006

Authors and Affiliations

  • C. M. Cabrera
    • 1
  • F. Cobo
    • 1
  • A. Nieto
    • 1
  • J. L. Cortés
    • 1
  • R. M. Montes
    • 1
  • P. Catalina
    • 1
  • A. Concha
    • 1
  1. 1.Stem Cell Bank of Andalucia (Spanish Central Node)Hospital Universitario Virgen de las NievesAvenida de las Fuerzas Armadas No. 2GranadaSpain

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