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Genetic structure and diversity of the Iberian populations of the freshwater blenny Salaria fluviatilis (Asso, 1801) and its conservation implications

  • Laura Méndez
  • Anabel PerdicesEmail author
  • Annie Machordom
Research Article

Abstract

The freshwater blenny Salaria fluviatilis has a circum-Mediterranean distribution, but it is restricted to highly specific habitats and is particularly susceptible to human disturbances, leading to the decline of Iberian populations of the species, resulting in their classification as Vulnerable. The aim of this study is to evaluate the genetic diversity and structure of the Iberian populations of S. fluviatilis to facilitate the design of robust conservation programs for the species. Potential barriers among populations and recent bottlenecks were also identified. Eleven Iberian populations (N = 337) of S. fluviatilis were genotyped for twelve polymorphic microsatellites. Our results showed strong genetic structure among Iberian populations, clustering them into four groups: north-eastern Iberian group (Fluviá, Segre, Matarraña and Cabriel rivers and Albufera Lagoon), southern Iberian group (Verde, Guadaíza and Guadalmansa rivers), Atlantic group (Zújar River and Ruidera Lakes) and Bañolas Lake (north-eastern Spain). Genetic diversity was higher in the north-eastern Iberian group (average observed heterozygosity Ho = 0.5 and number of alleles Na = 4.9) compared with in the southern Iberian group (average Ho = 0.42 and Na = 2.3), although the lowest observed heterozygosity was found in the Atlantic group (Ruidera Lakes, Ho = 0.324 ± 0.106). The analyses also revealed a recent bottleneck in the southern Iberian group. Our results not only provide essential information for future conservation plans of the Iberian populations of S. fluviatilis but also for the maintenance of the species’ genetic pool in general, for which the four clusters identified in this study should be considered.

Keywords

Fragmented populations Iberian Peninsula Microsatellites Peripheral populations Salaria (Blennius) fluviatilis 

Notes

Acknowledgements

We are grateful to F. Alonso, A. Pradillo, P. Risueño, D. Vinyoles, J. M. Ortíz, Q. Pou and J. Vukic for their assistance with the field work. S. Rubio provided most of the Southern samples (Centro de Cría Los Villares, Agencia Medio Ambiente y Agua, Junta de Andalucía). We thank P. Ochoa and R. García for their help in the laboratory, V. López and G. Sánchez-Montes for their assistance in the analyses, and V. González Cascón for her assistance in maps reconstruction. Thanks to Melinda Modrell who conscientiously revised the language.

Funding

Funding was provided by Ministerio de Economía y Competitividad (ES) (Grant No. CTM2014-57949-R).

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© Springer Nature B.V. 2019

Authors and Affiliations

  1. 1.German Centre for Integrative Biodiversity Research (iDiv) Halle-Jena-LeipzigLeipzigGermany
  2. 2.Department of Biodiversity and Evolutionary BiologyMuseo Nacional de Ciencias Naturales (MNCN-CSIC)MadridSpain

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