Hypermethylation-mediated inactivation of miR-124 predicts poor prognosis and promotes tumor growth at least partially through targeting EZH2/H3K27me3 in ESCC
Accumulating evidences indicated that some microRNAs (miRNAs) play a critical role during the carcinogenesis. In the present study, we found that miR-124 is down-regulated in esophageal squamous cell carcinoma (ESCC) tissues. Three miR-124 encoding genes, including mir-124-1, mir-124-2, and mir-124-3, harboring CpG islands undergo methylation-mediated miR-124 inactivation in ESCC tissues. The methylation status of all these three genes was negatively associated with the expression of miR-124. The low expression of miR-124 and the hypermethylation of mir-124-1 and mir-124-3 were associated with the clinico-pathological parameters indicating the poor prognosis. In addition, promoter methylation of all three genes plus low expression of miR-124 was the independent poor prognostic marker for ESCC patients. In conclusion, miR-124 may function as a tumor suppressive miRNA, and hypermethylation-mediated inactivation of miR-124 may be useful for a poor prognostic marker for ESCC patients.
KeywordsEsophageal squamous cell carcinoma miR-124 Methylation mir-124-1 mir-124-2 mir-124-3
Esophageal squamous cell carcinoma
3′ Untranslated regions
This work was supported by the Grants from the Ordinary University Considerable Distinctive Subjects Foundation of Hebei Province (No. 200552).
This work was supported by National Nature Science Foundation of China (No. 81001178), and Science Foundation of Hebei Province (No. 16967788D).
Compliance with ethical standards
Conflict of interest
The authors had no conflicts of interest to declare in relation to this article.
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