MiR-153 regulates cardiomyocyte apoptosis by targeting Nrf2/HO-1 signaling
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MicroRNAs (miRNAs) play various roles in the regulation of human disease, including cardiovascular diseases. MiR-153 has been previously shown to be involved in regulating neuron survival during cerebral ischemia/reperfusion (I/R) injury. However, whether miR-153 is involved in I/R-induced cardiomyocyte apoptosis remains to be elucidated. In this study, we aimed to explore the role of miR-153 in the regulation of I/R-induced cardiomyocyte apoptosis and to investigate the miR-153-mediated molecular signaling pathway responsible for its effect on cardiomyocytes using an oxygen-glucose deprivation and reoxygenation (OGD/R) cellular model. We found that OGD/R treatment induced significant upregulation of miR-153 in cardiomyocytes causing reactive oxygen species (ROS) production and cell apoptosis signaling activation and subsequently leading to cardiomyocyte apoptosis. Suppression of miR-153 protected cardiomyocytes against OGD/R treatment. We further identified that nuclear factor-like 2 (Nrf2) is a functional target of miR-153. Nrf2/ heme oxygenase-1 (HO-1) signaling plays a critical role in miR-153 regulated OGD/R-induced cardiomyocyte apoptosis. Our study indicates that the inhibition of miR-153 or restoration of Nrf2 may serve as a potential therapeutic strategy for ischemia/reperfusion injury prevention.
KeywordsIschemia/reperfusion miR-153 Nrf2 Cardiomyocytes Apoptosis
Oxygen-glucose deprivation and reoxygenation
Nuclear factor-like 2
XTZ and YLZ were contributed equally to this work. Conception and design: XTZ, YLZ, and WH. Collection and assembly of data: YLZ and WH. Data analysis and interpretation: YLZ and WH. Contribution of reagents, materials, and analysis tools wrote the paper: LG. All authors read and approved the final manuscript.
Compliance with ethical standards
Conflicts of interest
The authors declare that they have no conflict of interest.
Research involving human participants and/or animals
This study was approved by the Ethics Committee of the Yidu Central Hospital of Wei Fang.
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