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Chromosome Research

, Volume 26, Issue 4, pp 243–253 | Cite as

Clonal reproduction assured by sister chromosome pairing in dojo loach, a teleost fish

  • Masamichi KurodaEmail author
  • Takafumi Fujimoto
  • Masaru Murakami
  • Etsuro Yamaha
  • Katsutoshi Arai
Original Article
First Online:

Abstract

Wild-type dojo loach (Misgurnus anguillicaudatus) commonly reproduces bisexually as a gonochoristic diploid (2n = 50), but gynogenetically reproducing clonal diploid lines (2n = 50) exist in certain districts in Japan. Clones have been considered to develop from past hybridization event(s) between two genetically diverse groups, A and B, within the species. Fluorescence in situ hybridization analyses using the repetitive sequence “ManDra” as a probe clearly distinguished 25 chromosomes derived from group B out of a total of 50 diploid chromosomes of the clone, providing strong molecular cytogenetic evidence of its hybrid origin. In meiosis, diploid wild-type showed 25 bivalents, while diploid clones revealed 50 bivalents, indicating the presence of 100 chromosomes. In meiotic chromosome spreads in sex-reversed clonal males, ManDra signals were detected in 25 out of 50 bivalents, and only one out of two bivalents possessing major ribosomal RNA coding regions exhibited two positive ManDra signals. In clonal females, ManDra signals were detected in approximately 25 out of 50 bivalents. Thus, unreduced gametes should be generated by the pairing between sister chromosomes doubled from each ancestral chromosome from the different groups by premeiotic endomitosis. Sister chromosome pairing should assure production of unreduced isogenic clonal gametes due to the absence of the influence of recombination or crossing over.

Keywords

Clone FISH Hybrid Premeiotic endomitosis Sister chromosome Unreduced gametes 

Abbreviations

DAPI

4′, 6-Diamidino-2-phenylindole, dihydrochloride

FISH

Fluorescence in situ hybridization

GISH

Genomic in situ hybridization

GV

Germinal vesicle

IRBP2

Interphotoreceptor retinoid binding protein 2

mtDNA-CR

Mitochondrial DNA control region

NORs

Nucleolar organizing regions

RAG1

Recombination activating gene 1

rDNA

Ribosomal DNA

SSC

Saline sodium citrate buffer

tRNA

Transfer RNA

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Notes

Acknowledgments

We thank Dr. Atsushi Fujiwara (National Research Institute of Fisheries Science, Japan Fisheries Research and Education Agency) for technical advice. This study was supported by Grants-in-Aid from JSPS (Japan Society for the Promotion of Science) KAKENHI Grant Number 15H02457 and JSPS Research Fellow Number 17J01971.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Informed consent

Informed consent was obtained from all individual participants included in the study.

Ethical approval

This study was performed according to the Guide for the Care and Use of Laboratory Animals at Hokkaido University. All animal experiments were approved by the animal study ethical committee of Hokkaido University (approval number 19-2).

Supplementary material

10577_2018_9581_MOESM1_ESM.pdf (123 kb)
Table S1 (PDF 122 kb)
10577_2018_9581_MOESM2_ESM.pdf (111 kb)
Table S2 (PDF 110 kb)
10577_2018_9581_MOESM3_ESM.pdf (110 kb)
Table S3 (PDF 109 kb)
10577_2018_9581_MOESM4_ESM.pdf (114 kb)
Table S4 (PDF 113 kb)
10577_2018_9581_MOESM5_ESM.pdf (281 kb)
Fig. S1 (PDF 281 kb)

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© Springer Science+Business Media B.V., part of Springer Nature 2018

Authors and Affiliations

  1. 1.Faculty and Graduate School of Fisheries SciencesHokkaido UniversityHakodateJapan
  2. 2.Japan Society for the Promotion of ScienceTokyoJapan
  3. 3.School of Veterinary MedicineAzabu UniversitySagamiharaJapan
  4. 4.Nanae Fresh-Water Laboratory, Field Science Center for Northern BiosphereHokkaido UniversityNanaeJapan

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