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TLR4/MyD88/NF-κB-Mediated Inflammation Contributes to Cardiac Dysfunction in Rats of PTSD

  • Moujie Liu
  • Juhua XieEmail author
  • Yingxian SunEmail author
Original Research
  • 11 Downloads

Abstract

Post-traumatic stress disorder (PTSD) is related with myocardial injury and cardiac dysfunction, while the molecular mechanism has not been clear. This study investigated whether TLR4/MyD88/NF-κB-mediated inflammation involved in myocardial injury of PTSD. Adult male Wistar rats were exposed to single-prolonged stress (SPS), which was used broadly as a animal model of PTSD. Morris Water Maze (MWM) test and forced swimming test (FST) was carried out for behavioral testing. The protein expression of atrial natriuretic peptide (ANP) and brain natriuretic peptide (BNP) in the left ventricular of heart and TLR4/MyD88/NF-κB-mediated inflammation were examined. Our results showed that there were obvious increased in the protein expression of ANP and BNP in heart after exposure to SPS, SPS also significantly enhanced the serum level of IL-1β and TNF-α, and meanwhile, the TLR4/MyD88/NF-κB pathway were activated. These results demonstrated that the TLR4/MyD88/NF-κB pathway were involved in the myocardial injury of PTSD, which might be one of possible molecular mechanism contributed to the pathogenesis of cardiac dysfunction in PTSD.

Keywords

Single-prolonged stress Post-traumatic stress disorder TLR4 MyD88 Nf-κb 

Notes

Acknowledgements

This work was supported by Doctoral Scientific Research Fund from Liao Ning Province (No. 20170520016); Doctoral Scientific Research Fund from Shenyang Medical College (No. 20163047; No. 20171005 and No. 20181005). The authors thank the Laboratory of Cardiology in the First Hospital of China Medical University for valuable help in this experiment.

Author Contributions

All authors mentioned in the paper have significantly contributed to the research. ML and YS conceived and designed the experiments, ML and JX performed the experiment and wrote the article, YS refined the article. All authors read and approved the final manuscript.

Compliance with Ethical Standards

Conflict of interest

The authors declare no potential conflicts of interest.

Ethical Approval

All applicable international, national, and/or institutional guidelines for the care and use of animals were followed.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  1. 1.Department of CardiologyThe First Hospital of China Medical UniversityShenyangPeople’s Republic of China
  2. 2.Department of Histology and Embryology, Basic Medical Sciences CollegeShenyang Medical CollegeShenyangPeople’s Republic of China

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