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Artemisia amygdalina Upregulates Nrf2 and Protects Neurons Against Oxidative Stress in Alzheimer Disease

  • Nasreena Sajjad
  • Abubakar Wani
  • Ankita Sharma
  • Rohaya Ali
  • Sumaya Hassan
  • Rabia Hamid
  • Huma Habib
  • Bashir Ahmad GanaiEmail author
Original Research
  • 37 Downloads

Abstract

Alzheimer disease is a complex neurodegenerative disorder. It is the common form of dementia in elderly people. The etiology of this disease is multifactorial, pathologically it is accompanied with accumulation of amyloid beta and neurofibrillary tangles. Accumulation of amyloid beta and mitochondrial dysfunction leads to oxidative stress. In this study, neuroprotective effect of Artemisiaamygdalina against H2O2-induced death was studied in differentiated N2a and SH-SY5Ycells. Cells were treated with H2O2 to induce toxicity which was attenuated by Artemisia amygdalina. The nuclear factor erythroid 2-related factor 2 (Nrf2) is an emerging regulator of cellular resistance to oxidants. It controls the basal and induced expression of antioxidant response element-dependent genes. Further, we demonstrated that Artemisia amygdalina protects neurons through upregulation of Nrf2 pathway. Moreover, reactive oxygen species and mitochondrial membrane potential loss formed by H2O2 was attenuated by Artemisia amygdalina. Thus, Artemisia amygdalina may have the possibility to be a therapeutic agent for Alzheimer disease.

Keywords

Artemisia amygdalina Antioxidant Oxidative stress Neuroprotection Nrf2 

Notes

Acknowledgements

The authors acknowledge the support from University Grants Commission.

Author Contributions

NS has designed and performed the experiments and wrote the manuscript. ABW performed few experiments. RA helped with some experiments. SH carried few in vitro assays. AS and HH edited revised manuscript. RH and BAG supervised the findings of this work and reviewed the final manuscript.

Compliance with Ethical Standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical Approval

This article does not contain any studies with human participants or animals performed by any of the authors.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of BiochemistryUniversity of KashmirSrinagarIndia
  2. 2.PK-PD- Toxicology and FormulationCSIR- Indian Institute of Integrative MedicineJammuIndia
  3. 3.Centre of Research for DevelopmentUniversity of KashmirSrinagarIndia
  4. 4.Department of BiochemistryIslamia College of Science and CommerceSrinagarIndia

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