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Expression Pattern of Long Non-coding RNAs in Schizophrenic Patients

  • Mohammad Reza Safari
  • Alireza Komaki
  • Shahram Arsang-Jang
  • Mohammad TaheriEmail author
  • Soudeh Ghafouri-FardEmail author
Original Research
  • 47 Downloads

Abstract

The role of long non-coding RNAs (lncRNAs) in the pathogenesis of neurological disorders including schizophrenia has been highlighted by independent studies. In the present study, we compared peripheral blood expression of seven lncRNAs between schizophrenic patients and sex- and age-matched controls using quantitative real-time PCR technique. FAS-AS1, PVT1 and TUG1 were significantly down-regulated in schizophrenic patients compared with healthy individuals (P = 0.007, 0.003 and 0.001, respectively). The association between FAS-AS1 expression and schizophrenia was significant in male subjects aged more than 50 but not in other subgroups. GAS5, NEAT1 and OIP5-AS1 expressions were not significantly different between patients and controls (P = 0.523, 0.739 and 0.267, respectively). The associations between GAS5, NEAT1 and OIP5-AS1 expressions and schizophrenia were significant in female subjects but not in male subjects. THRIL was up-regulated in schizophrenic patients compared with healthy subjects. Based on the results of bootstraped median regression, and after controlling for the effects of age and sex, the difference in its expression between cases and controls was significant (P = 0.014), while the interaction between group and sex was not significant. The expression of lncRNAs was not correlated with age in any study subgroups. In addition, we found sex-based pairwise correlations between PVT1 expression and expression levels of OIP5-AS1, THRIL and NEAT1. We also demonstrated high sensitivity and specificity of GAS5 for diagnosis of schizophrenia in female patients. The current study provides further evidence for the participation of lncRNAs in the pathogenesis of schizophrenia. Future studies are needed to confirm the suitability of lncRNAs as peripheral biomarkers for this psychiatric disorder.

Keywords

Schizophrenia FAS-AS1 PVT1 TUG1 OIP5-AS1 THRIL NEAT1 GAS5 

Notes

Author Contributions

SGF wrote the manuscript. SAJ analyzed the data. MT and AK supervised the study. MRS performed the laboratory tests.

Funding

This study was financially supported by Hamadan University of Medical Sciences (Grant Number 970121264).

Compliance with Ethical Standards

Conflict of interest

The authors declare they have no conflict of interest.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Neurophysiology Research CenterHamadan University of Medical SciencesHamadanIran
  2. 2.Clinical Research Development Center (CRDU)Qom University of Medical SciencesQomIran
  3. 3.Student Research CommitteeShahid Beheshti University of Medical SciencesTehranIran
  4. 4.Urogenital Stem Cell Research CenterShahid Beheshti University of Medical SciencesTehranIran
  5. 5.Department of Medical GeneticsShahid Beheshti University of Medical SciencesTehranIran

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