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Isolation and characterization of cellulosic fibers from ramie using organosolv degumming process

  • Yongshuai Qu
  • Weilun Yin
  • RuiYun ZhangEmail author
  • Shuyuan Zhao
  • Liu Liu
  • Jianyong Yu
Original Research


Degumming bast fibers by organic solvents has been a promising method in recent years due to easy recovery and reuse of organic solvents. In this research, the possibility of ramie fiber degumming by glycol and a combination of acetic acid with glycol was studied, in which two steps were involved in the degumming process: distilled water boiling pretreatment and organosolv treatment by a combination of glycol/acetic acid (100/0, 90/10, 80/20, 70/30, 60/40, 50/50). Results displayed that the pretreatment could remove 6.99% of hemicellulose, 0.59% of lignin and 36.26% of other gums compared with raw ramie. While with organosolv treatment (130 °C, 6 h), fibers treated by glycol/acetic acid (50/50) had the best effect of removing gums. The hemicellulose and lignin content of fibers reduced by 44.81% and 54.12%, respectively (compared with raw ramie), while the residual gum content still failed to meet the requirements of spinning process. Besides, the tenacity of glycol/acetic acid treated fibers was lower than that of only glycol treated fibers (4.67 cN/dtex). Considering that the addition of acid could cause a decrease in fiber tenacity, the step of organosolv (only glycol) treatment was optimized by altering the degumming condition. The tenacity, linear density, non-cellulosic component ratio of fibers treated with the optimized condition (200 °C, 80 min) were 6.53 cN/dtex, 6.06 dtex, 5.78%, respectively, which met the needs of industrial production. Compared with the organosolv treated fibers, these properties of fibers with traditional alkaline treatment were better, but the yield (62.4%) was much lower than that of fibers treated with glycol in two degumming condition (77–82%). Considering impressive properties of the treated ramie, the method of organosolv degumming with high degumming efficiency and environmental protection would bring an innovative thought for natural fiber isolation.

Graphic abstract


Ramie fiber Acetic acid-glycol Glycol Organosolv degumming 



The authors acknowledge the following financial support for the research and authorship of this article: This work was supported by the Fundamental Research Funds for the Central Universities (Grant Number EG2018006), the Shanghai Municipal Natural Science Foundation (Grant Number 14 ZR1401000) and the Fundamental Research Funds for the Central Universities (Grant Number CUSFDH-D-2017014).

Compliance with ethical standards

Conflict of interest

The authors declare no conflicts of interest.


  1. Amiralian N, Annamalai PK, Memmott P, Martin DJ (2015) Isolation of cellulose nanofibrils from Triodia pungens via different mechanical methods. Cellulose 22:2483–2498CrossRefGoogle Scholar
  2. Aslan M, Sørensen BF, Bo M (2011) Strength variability of single flax fibres. J Mater Sci 46:6344–6354CrossRefGoogle Scholar
  3. Balaji AN, Nagarajan KJ (2017) Characterization of alkali treated and untreated new cellulosic fiber from Saharan aloe vera cactus leaves. Carbohydr Polym 174:200CrossRefGoogle Scholar
  4. Bledzki A, Gassan J (1999) Composites reinforced with cellulose based fibres. Prog Polym Sci 24:221–274CrossRefGoogle Scholar
  5. Bozell JJ, Black SK, Myers M, Cahill D, Miller WP, Park S (2011) Solvent fractionation of renewable woody feedstocks: organosolv generation of biorefinery process streams for the production of biobased chemicals. Biomass Bioenergy 35:4197–4208CrossRefGoogle Scholar
  6. Carvalho DMD, Queiroz JHD, Colodette JL (2016) Assessment of alkaline pretreatment for the production of bioethanol from eucalyptus, sugarcane bagasse and sugarcane straw. Ind Crops Prod 94:932–941CrossRefGoogle Scholar
  7. Cheng F, Zhao X, Hu Y (2018) Lignocellulosic biomass delignification using aqueous alcohol solutions with the catalysis of acidic ionic liquids: a comparison study of solvents. Bioresour Technol 249:969–975PubMedCrossRefPubMedCentralGoogle Scholar
  8. Chirayil CJ, Joy J, Mathew L, Mozetic M, Koetz J, Thomas S (2014) Isolation and characterization of cellulose nanofibrils from Helicteres isora plant. Ind Crops Prod 59:27–34CrossRefGoogle Scholar
  9. Choi HY, Lee JS (2012) Effects of surface treatment of ramie fibers in a ramie/poly(lactic acid) composite. Fibers Polym 13:217–223CrossRefGoogle Scholar
  10. Dapía S, Santos V, Parajó JC (2002) Study of formic acid as an agent for biomass fractionation. Biomass Bioenergy 22:213–221CrossRefGoogle Scholar
  11. Dehbari N, Tavakoli J, Zhao J, Tang Y (2017) In situ formed internal water channels improving water swelling and mechanical properties of water swellable rubber composites. J Appl Polym Sci 134:44548CrossRefGoogle Scholar
  12. Deng L et al (2012) Effect of chemical and biological degumming on the adsorption of heavy metal by cellulose xanthogenates prepared from Eichhornia crassipes. Bioresour Technol 107:41–45PubMedCrossRefPubMedCentralGoogle Scholar
  13. Du L, Wang J, Zhang Y, Qi C, Wolcott MP, Yu Z (2017) A co-production of sugars, lignosulfonates, cellulose, and cellulose nanocrystals from ball-milled woods. Bioresour Technol 238:254–262PubMedCrossRefPubMedCentralGoogle Scholar
  14. El Achaby M, El Miri N, Hannache H, Gmouh S, Trabadelo V, Aboulkas A, Youcef HB (2018) Cellulose nanocrystals from Miscanthus fibers: insights into rheological, physico-chemical properties and polymer reinforcing ability. Cellulose 25:6603–6619CrossRefGoogle Scholar
  15. Fahma F, Iwamoto S, Hori N, Iwata T, Takemura A (2011) Effect of pre-acid-hydrolysis treatment on morphology and properties of cellulose nanowhiskers from coconut husk. Cellulose 18:443–450CrossRefGoogle Scholar
  16. Faix O (1991) Classification of lignins from different botanical origins by FT-IR spectroscopy. Holzforschung 45:21–28CrossRefGoogle Scholar
  17. Fan X-S, Liu Z-W, Liu Z-T, Lu J (2010) A novel chemical degumming process for ramie bast fiber. Text Res J 80:2046–2051CrossRefGoogle Scholar
  18. Fernandez EO, Young RA (1996) Properties of cellulose pulps from acidic and basic processes. Cellulose 3:21–44CrossRefGoogle Scholar
  19. Fernández N, Mörck R, Johnsrud SC, Kringstad KP (1990) Carbon-13 NMR study on lignin from bagasse. Holzforschung 44:35–38CrossRefGoogle Scholar
  20. Ferraz A, Mendonça R, da Silva FT (2000) Organosolv delignification of white-and brown-rotted Eucalyptus grandis hardwood. J Chem Technol Biotechnol 75:18–24CrossRefGoogle Scholar
  21. Ferrer A, Vega A, Rodrã-Guez A, Jiménez L (2013) Acetosolv pulping for the fractionation of empty fruit bunches from palm oil industry. Bioresour Technol 132:115–120PubMedCrossRefPubMedCentralGoogle Scholar
  22. French AD (2014) Idealized powder diffraction patterns for cellulose polymorphs. Cellulose 21:885–896CrossRefGoogle Scholar
  23. Gu J, Catchmark JM (2012) Impact of hemicelluloses and pectin on sphere-like bacterial cellulose assembly. Carbohydr Polym 88:547–557CrossRefGoogle Scholar
  24. Hao J, Xu S, Xu N, Li D, Linhardt RJ, Zhang Z (2017) Impact of degree of oxidation on the physicochemical properties of microcrystalline cellulose. Carbohydr Polym 155:483–490PubMedCrossRefPubMedCentralGoogle Scholar
  25. Hubbell CA, Ragauskas AJ (2010) Effect of acid-chlorite delignification on cellulose degree of polymerization. Bioresour Technol 101:7410–7415PubMedCrossRefPubMedCentralGoogle Scholar
  26. Izydorczyk MS, Biliaderis CG (1995) Cereal arabinoxylans: advances in structure and physicochemical properties. Carbohydr Polym 28:33–48CrossRefGoogle Scholar
  27. Jiang W et al (2018) A green degumming process of ramie. Ind Crops Prod 120:131–134CrossRefGoogle Scholar
  28. Kang SY, Epps HH (2009) Effect of scouring and enzyme treatment on moisture regain percentage of naturally colored cottons. J Text Inst 100:598–606CrossRefGoogle Scholar
  29. Kassab Z, Boujemaoui A, Youcef HB, Hajlane A, Hannache H, El Achaby M (2019) Production of cellulose nanofibrils from alfa fibers and its nanoreinforcement potential in polymer nanocomposites. Cellulose 26:1–15CrossRefGoogle Scholar
  30. Keshk SMAS (2015) Effect of different alkaline solutions on crystalline structure of cellulose at different temperatures. Carbohydr Polym 115:658–662PubMedCrossRefPubMedCentralGoogle Scholar
  31. Li Z, Yu C (2014) Effect of peroxide and softness modification on properties of ramie fiber. Fibers Polym 15:2105–2111. CrossRefGoogle Scholar
  32. Li Z et al (2016a) High-efficiency ramie fiber degumming and self-powered degumming wastewater treatment using triboelectric nanogenerator. Nano Energy 22:548–557CrossRefGoogle Scholar
  33. Li Z, Meng C, Zhou J, Li Z, Ding J, Liu F, Yu C (2016b) Characterization and control of oxidized cellulose in ramie fibers during oxidative degumming. Text Res J 87:1828–1840. CrossRefGoogle Scholar
  34. Liu L, Xiang Y, Zhang R, Li B, Yu J (2017) Effect of NaClO dosage on the structure of degummed hemp fibers by 2,2,6,6-tetramethyl-1-piperidinyloxy-laccase degumming. Text Res J. CrossRefGoogle Scholar
  35. Maache M, Bezazi A, Amroune S, Scarpa F, Dufresne A (2017) Characterization of a novel natural cellulosic fiber from Juncus effusus L. Carbohydr Polym 171:163–172PubMedCrossRefPubMedCentralGoogle Scholar
  36. McDonough TJ (1992) The chemistry of organosolv delignification. TAPPI Solvent Pulping SeminarGoogle Scholar
  37. Meng C, Li Z, Wang C, Yu C (2016) Sustained-release alkali source used in the oxidation degumming of ramie. Text Res J 87:1155–1164. CrossRefGoogle Scholar
  38. Meng C, Yang J, Zhang B, Yu C (2018) Rapid and energy-saving preparation of ramie fiber in TEMPO-mediated selective oxidation system. Ind Crops Prod 126:143–150CrossRefGoogle Scholar
  39. Mukhopadhyay A, Dutta N, Chattopadhyay D, Chakrabarti K (2013) Degumming of ramie fiber and the production of reducing sugars from waste peels using nanoparticle supplemented pectate lyase. Bioresour Technol 137:202–208PubMedCrossRefPubMedCentralGoogle Scholar
  40. Nishiyama Y, Langan P, Chanzy H (2002) Crystal structure and hydrogen-bonding system in cellulose Iβ from synchrotron X-ray and neutron fiber diffraction. J Am Chem Soc 124:9074–9082PubMedCrossRefPubMedCentralGoogle Scholar
  41. Przybysz P, Kucner MA, Dubowik M, Przybysz K (2017) Laboratory refining of bleached softwood kraft pulp in water and a series of alcohols of different molecular weights and polarities: effects on swelling and fiber length. BioResources 12:1737–1748CrossRefGoogle Scholar
  42. Rodríguez A, Jiménez L (2008) Pulping with organic solvents other than alcohols. Afinidad LXV 65:188–196Google Scholar
  43. Romaní A, Garrote G, López F, Parajó JC (2011) Eucalyptus globulus wood fractionation by autohydrolysis and organosolv delignification. Bioresour Technol 102:5896–5904PubMedCrossRefPubMedCentralGoogle Scholar
  44. Sarkanen KV (1980) Acid-catalyzed delignification of lignocellulosics in organic solvents. Prog Biomass Convers 2:127–144CrossRefGoogle Scholar
  45. Schwanninger M, Rodrigues JC, Pereira H, Hinterstoisser B (2004) Effects of short-time vibratory ball milling on the shape of FT-IR spectra of wood and cellulose. Vib Spectrosc 36:23–40CrossRefGoogle Scholar
  46. Shen M, Wang L, Long JJ (2015) Biodegumming of ramie fiber with pectinases enhanced by oxygen plasma. J Clean Prod 101:395–403CrossRefGoogle Scholar
  47. Song Y, Jiang W, Zhang Y, Ben H, Han G, Ragauskas AJ (2018) Isolation and characterization of cellulosic fibers from kenaf bast using steam explosion and Fenton oxidation treatment. Cellulose 25:4979–4992CrossRefGoogle Scholar
  48. Sun R, Lawther JM, Banks W (1996) Fractional and structural characterization of wheat straw hemicelluloses. Carbohydr Polym 29:325–331CrossRefGoogle Scholar
  49. Wickholm K, Larsson PT, Iversen T (1998) Assignment of non-crystalline forms in cellulose I by CP/MAS 13 C NMR spectroscopy. Carbohydr Res 312:123–129CrossRefGoogle Scholar
  50. Xu F et al (2006) Characterisation of degraded organosolv hemicelluloses from wheat straw. Polym Degrad Stab 91:1880–1886CrossRefGoogle Scholar
  51. Xuebing Z, Keke C, Dehua L (2009) Organosolv pretreatment of lignocellulosic biomass for enzymatic hydrolysis. Appl Microbiol Biotechnol 82:815–827CrossRefGoogle Scholar
  52. Yang B, Wyman CE (2008) Pretreatment: the key to unlocking low-cost cellulosic ethanol. Biofuels Bioprod Biorefin 2:26–40CrossRefGoogle Scholar
  53. Yawalata D (2001) Catalytic selectivity in alcohol organosolv pulping of spruce wood. University of British Columbia, VancouverGoogle Scholar
  54. Yeping X, Jianyong Y, Liu L, Ruiyun Z, Yongshuai Q, Miaolei J (2018) The chemo-enzymatic modification and degumming of hemp fiber by the laccase-2,2,6,6-tetramethylpiperidine-1-oxyl radical-hemicellulase system and physico-chemical properties of the products. Text Res J. CrossRefGoogle Scholar
  55. Yu T, Ren J, Li S, Yuan H, Li Y (2010) Effect of fiber surface-treatments on the properties of poly(lactic acid)/ramie composites. Compos Part A 41:499–505CrossRefGoogle Scholar
  56. Yuan J, Yu Y, Wang Q, Fan X, Chen S, Wang P (2013) Modification of ramie with 1-butyl-3-methylimidazolium chloride ionic liquid. Fibers Polym 14:1254–1260CrossRefGoogle Scholar
  57. Yunos NSHM et al (2016) Enhanced oil recovery and lignocellulosic quality from oil palm biomass using combined pretreatment with compressed water and steam. J Clean Prod 142:S0959652616316882Google Scholar
  58. Zafeiropoulos NE, Vickers PE, Baillie CA, Watts JF (2003) An experimental investigation of modified and unmodified flax fibres with XPS, ToF-SIMS and ATR-FTIR. J Mater Sci 38:3903–3914CrossRefGoogle Scholar
  59. Zhang J, Zhang J (2010) Effect of refined processing on the physical and chemical properties of hemp bast fibers. Text Res J 80:744–753CrossRefGoogle Scholar
  60. Zhang Y et al (2018) One-step fractionation of the main components of bamboo by formic acid-based organosolv process under pressure. J Wood Chem Technol. CrossRefGoogle Scholar
  61. ZhanYing Z, Harrison MD, Rackemann DW, Doherty WOS, O’Hara IM (2016) Organosolv pretreatment of plant biomass for enhanced enzymatic saccharification. Green Chem 18:360–381CrossRefGoogle Scholar
  62. Zheng L, Du Y, Zhang J (2001) Degumming of ramie fibers by alkalophilic bacteria and their polysaccharide-degrading enzymes. Bioresour Technol 78:89–94PubMedCrossRefPubMedCentralGoogle Scholar

Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  • Yongshuai Qu
    • 1
  • Weilun Yin
    • 1
  • RuiYun Zhang
    • 1
    • 2
    Email author
  • Shuyuan Zhao
    • 1
  • Liu Liu
    • 2
  • Jianyong Yu
    • 2
  1. 1.Key Laboratory of Textile Science and Technology, Ministry of Education, College of TextilesDonghua UniversityShanghaiChina
  2. 2.Innovation Center for Textile Science and TechnologyDonghua UniversityShanghaiChina

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