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Facile synthesis of Cu2+-immobilized imprinted cotton for the selective adsorption of bovine hemoglobin

  • Pei Wang
  • Yankun Yin
  • Jing Xu
  • Shaohua ChenEmail author
  • Hua Wang
Original Research


Degreasing cotton showing selectivity for the bovine hemoglobin (BHb) was developed by an imprinting procedure that is based on metal coordinate interaction. The morphological structure of the as-prepared samples was characterized by Scanning Electron Microscopy, and the chemical modification steps were characterized by Fourier Transform Infrared Spectroscopy. The results of adsorption experiments show that the maximum adsorption capacity of the Cu2+-immobilized molecularly imprinted cotton (Cu2+-MIC) and Cu2+-immobilized non-imprinted cotton (Cu2+-NIC) was 140.33 mg/g and 17.78 mg/g, respectively, at the optimum pH value of 6.2. The adsorption process followed a pseudo-second-order kinetic and the adsorption equilibrium could be achieved in 30 min. The adsorption isotherm data could be well described by a Langmuir model. Moreover, satisfactory reusability is demonstrated by five adsorption–desorption cycles with no significant decrease of the adsorption capacity. The SDS-PAGE analysis demonstrated that the Cu2+-MIC could be applied successfully in separation of BHb from the bovine blood sample. This procedure presents facile, cheap and stable fabrication strategy for efficient separation of proteins.


Degreasing cotton Metal coordinate interaction Molecularly imprinted Bovine hemoglobin 


Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.


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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  1. 1.Key Laboratory of Textile Fibers and Products, Ministry of Education, P. R. C, College of Materials Science and EngineeringWuhan Textile UniversityWuhanChina
  2. 2.High-Tech Organic Fibers Key Laboratory of Sichuan ProvinceSichuan Textile Research InstituteChengduChina

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