Characterization, in vitro antibacterial activity, and toxicity for rat of tetracycline in a nanocomposite hydrogel based on PEG and cellulose Original Research First Online: 16 October 2019 Abstract
Hydrogels are among the drug delivery systems that are used to modify drug release by the oral route. Inclusion of porous nanoparticles and cellulose nanofibers (CNF) in a hydrogel matrix structure improves the mechanical strength of the hydrogel and modifies drug release. CNF have been widely used for the preparation of biomedical systems because of low toxicity, biodegradability, and biocompatibility. Besides, a positive influence on mechanical and physical resistance is shown. In this study, nanocomposite hydrogels containing polyethylene glycol, Acrylamide, N, N′-methylene bis-acrylamide, and CNF are formulated, and then tetracycline was loaded into the hydrogels. Tetracycline release was measured using UV spectrometer. Morphology and microscopic structure of synthesized nanocomposites are studied using FE-SEM, XRD, and FTIR analyses. Moreover, the antibacterial activity of tetracycline nanocomposite hydrogels against
Staphylococcus aureus and Escherichia coli was tested. Nanocomposite hydrogel oral toxicity test was performed in adult male Wistar rats. The results showed that the formulation has no significant statistical effect on the behavioral pattern, body weight, and clinical parameters of the experimental animals. Furthermore, pathological examination showed the normal structure of stomach and intestine. Antibacterial activity study showed that Staphylococcus aureus and E. Coli are sensitive to the formulated compound 3. Therefore, these formulations can be considered for future as oral drug delivery systems. Keywords Cellulose nanofiber Hydrogel Modified release Nanocomposite Polyethylene glycol Tetracycline Notes Acknowledgments
The authors would like to appreciate Baqiyatallah University of Medical Sciences for support of the study and Professor Ali Khamesipour for his language comments and Dr. Sadegh Jamalkandi Azimzadeh for preparing figures.
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Ahmadi R, Kalbasi-Ashtari A, Oromiehie A, Yarmand M-S, Jahandideh F (2012) Development and characterization of a novel biodegradable edible film obtained from psyllium seed (
Plantago ovata Forsk
). J Food Eng 109:745–751.
https://doi.org/10.1016/j.jfoodeng.2011.11.010 CrossRef Google Scholar
Ahmed EM (2015) Hydrogel: preparation, characterization, and applications: a review. J Adv Res 6:105–121.
https://doi.org/10.1016/j.jare.2013.07.006 CrossRef PubMed Google Scholar
Atyabi F, Majzoob S, Iman M, Salehi M, Dorkoosh F (2005) In vitro evaluation and modification of pectinate gel beads containing trimethyl chitosan, as a multi-particulate system for delivery of water-soluble macromolecules to colon. Carbohyd Polym 61:39–51.
https://doi.org/10.1016/j.carbpol.2005.02.005 CrossRef Google Scholar
Barati A (2010) Nano-composite superabsorbent containing fertilizer nutrients used in agriculture. US Pat App. 12/701,613 Google Patents
Barati A, Norouzi H, Sharafoddinzadeh S, Davarnejad R (2010) Swelling kinetics modeling of cationic methacrylamide-based hydrogels. World Appl Sci J 11:1336–1341
Burt A (2015) Roger Cotton Histopathology Prize 2014. Histopathology 66:909
CrossRef Google Scholar
Caldorera-Moore M, Kang MK, Moore Z, Singh V, Sreenivasan S, Shi L et al (2011) Swelling behavior of nanoscale, shape-and size-specific, hydrogel particles fabricated using imprint lithography. Soft Matter 7:2879–2887.
https://doi.org/10.1039/C0SM01185A CrossRef Google Scholar
Caldorera-Moore M, Maass K, Hegab R, Fletcher G, Peppas N (2015) Hybrid responsive hydrogel carriers for oral delivery of low molecular weight therapeutic agents. J Drug Deliv Sci Tec 30:352–359.
https://doi.org/10.1016/j.jddst.2015.07.023 CrossRef Google Scholar
Carlson WB, Phelan GD (2012) Acrylamide hydrogels for tissue engineering. US Pat App. 13/879,976 Google Patents
Chen Y, Gao Y, de Silva LP, Pirraco RP, Ma M, Yang L, Reis RL, Chen J (2018) A thermos-/pH-responsive hydrogel (PNIPAM-PDMA-PAA) with diverse nanostructures and gel behaviors as a general drug carrier for drug release. Polym Chem 9:4063–4072.
https://doi.org/10.1039/C8PY00838H CrossRef Google Scholar
Dong Y, Paukkonen H, Fang W, Kontturi E, Laaksonen T, Laaksonen P (2018) Entangled and colloidally stable microcrystalline cellulose matrices in controlleddrug release. Int J Pharmaceut 548:113–119.
https://doi.org/10.1016/j.ijpharm.2018.06.022 CrossRef Google Scholar
Ekebafe L, Ogbeifun D, Okieimen F (2011) Polymer applications in agriculture. Biokemistri 23:81–89
French AD (2014) Idealized powder diffraction patterns for cellulose polymorphs. Cellulose 21:885–896.
https://doi.org/10.1007/s10570-013-0030-4 CrossRef Google Scholar
French AD, Santiago Cintrón M (2013) Cellulose polymorphy, crystallite size, and the Segal crystallinity index. Cellulose 20:583–588.
https://doi.org/10.1007/s10570-012-9833-y CrossRef Google Scholar
Fu L, Chen S, Yi J, Hou Z (2014) Effects of different fermentation methods on bacterial cellulose and acid production by Gluconacetobacter xylinus in Cantonese-style rice vinegar. Food Sci Technol Int 20:321–331.
https://doi.org/10.1177/1082013213486663 CrossRef PubMed Google Scholar
Fu X, Hosta-Rigau L, Chandrawati R, Cui J (2018) Multi-stimuli-responsive polymer particles, films, and hydrogels for drug delivery. Chem 4:2084–2107.
https://doi.org/10.1016/j.chempr.2018.07.002 CrossRef Google Scholar
Fu L, Qi C, Ma M, Wan P (2019) Multifunctional cellulose-based hydrogels for biomedical applications. J Mater Chem-B 7:1541–1562.
https://doi.org/10.1039/C8TB02331J CrossRef Google Scholar
Guo J, Catchmark JM (2012) Surface area and porosity of acid hydrolyzed cellulose nanowhiskers and cellulose produced by
. Carbohydr Polym 87(2):1026–1037.
https://doi.org/10.1016/j.carbpol.2011.07.060 CrossRef Google Scholar
Hakuta T, Shinzawa H, Ozaki Y (2009) Practical method for the detection of tetracyclines in honey by HPLC and derivative UV-vis spectra. Anal Sci 25:1149–1153.
https://doi.org/10.2116/analsci.25.1149 CrossRef PubMed Google Scholar
Hu X, Wang J, Huang H (2013) Impacts of some macromolecules on the characteristics of hydrogels prepared from pineapple peel cellulose using ionic liquid. Cellulose 20:2923–2933.
https://doi.org/10.1007/s10570-013-0075-4 CrossRef Google Scholar
Huh AJ, Kwon YJ (2011) “Nanoantibiotics”: a new paradigm for treating infectious diseases using nanomaterials in the antibiotics resistant era. J Control Release 156:128–145.
https://doi.org/10.1016/j.jconrel.2011.07.002 CrossRef PubMed Google Scholar
Ibrahim MM, El-Zawawy WK (2015) Extraction of cellulose nanofibers from cotton linter and their composites. Handbook of polymer nanocomposites processing, performance and application. Springer, Berlin, pp 145–164
Isogai A, Bergstrom L (2018) Preparation of cellulose nanofibers using green and sustainable chemistry. Curr Opin Green Sustain Chem 12:15–21.
https://doi.org/10.1016/j.cogsc.2018.04.008 CrossRef Google Scholar
Joshy KS, Snigdha S, George A, Kalarikkal N, Pothen LA, Thomas S (2017) Core-shell nanoparticles of carboxy methyl cellulose and compritol-PEG for antiretroviral drug delivery. Cellulose 24:4759–4771.
https://doi.org/10.1007/s10570-017-1446-z CrossRef Google Scholar
Kost J, Langer R (2012) Responsive polymeric delivery systems. Adv Drug Deliv Rev 64:327–341.
https://doi.org/10.1016/j.addr.2012.09.014 CrossRef Google Scholar
Ling Z, Wang T, Makarem M, Santiago Cintrón M, Cheng HN, Kang X et al (2019) Effects of ball milling on the structure of cotton cellulose. Cellulose 26:305–328.
https://doi.org/10.1007/s10570-018-02230-x CrossRef Google Scholar
Liu H, Wang C, Li C, Qin Y, Wang Z, Yang F, Li Z, Wang J (2018) A functional chitosan-based hydrogel as a wound dressing and drug delivery system in the treatment of wound healing. RSC Adv 8:7533–7549.
https://doi.org/10.1039/C7RA13510F CrossRef Google Scholar
Lu J, Zhu W, Dai L, Si C, Ni Y (2019) Fabrication of thermos- and pH-sensitive cellulose nanofibrils reinforced hydrogel with biomass nanoparticles. Carbohyd Polym 215:289–295.
https://doi.org/10.1016/j.carbpol.2019.03.100 CrossRef Google Scholar
Mahinroosta M, Jomeh Farsangi Z, Allahverdi A, Shakoori Z (2018) Hydrogels as intelligent materials: a brief review of synthesis, properties and applications. Mater Today Chem 8:42–55.
https://doi.org/10.1016/j.mtchem.2018.02.004 CrossRef Google Scholar
Niu J, Wang J, Dai X, Shao Z, Huang Z (2018) Dual physically crosslinked healable polyacrylamide/cellulose nanofibers nanocomposite hydrogels with excellent mechanical properties. Carbohy Polym 193:73–81.
https://doi.org/10.1016/j.carbpol.2018.03.086 CrossRef Google Scholar
Ristic T, Zabret A, Zemljic LF, Persin Z (2017) Chitosan nanoparticles as a potential drug delivery system attached to viscose cellulose fibers. Cellulose 24:739–753.
https://doi.org/10.1007/s10570-016-1125-5 CrossRef Google Scholar
Segal L, Creely JJ, Martin AE, Conrad CM (1959) An empirical method for estimating the degree of crystallinity of native cellulose using the X-ray diffractometer. Text Res J 29(10):786–794.
https://doi.org/10.1177/004051755902901003 CrossRef Google Scholar
Shalla AH, Bhat MA, Yaseen Z (2018) Hydrogels for removal of recalcitrant organic dyes: a conceptual overview. J Environ Chem Eng 6:5938–5949.
https://doi.org/10.1016/j.jece.2018.08.063 CrossRef Google Scholar
Shunmugaperumal T, Kaur V, Thenrajan RS (2015) Lipid-and polymer-based drug delivery carriers for eradicating microbial biofilms causing medical device-related infections. Biofilm-based healthcare-associated infections. Springer, Berlin, pp 147–189
Spagnol C, Rodrigues FH, Neto AG, Pereira AG, Fajardo AR, Radovanovic E et al (2012) Nanocomposites based on poly (acrylamide-co-acrylate) and cellulose nanowhiskers. Eur Polym J 48:454–463.
https://doi.org/10.1016/j.eurpolymj.2011.12.005 CrossRef Google Scholar
Taghizadeh MT, Ashassi-Sorkhabi H, Afkari R, Kazempour A (2019) Cross-linked chitosan in nano and bead scales as drug carriers for betamethasone and tetracycline. Int J Biol Macromol 131:581–588.
https://doi.org/10.1016/j.ijbiomac.2019.03.094 CrossRef PubMed Google Scholar
Wada M, Okano T (2001) Localization of Iα and Iβ phases in algal cellulose revealed by acid treatments. Cellulose 8:183–188.
https://doi.org/10.1023/A:1013196220602 CrossRef Google Scholar
Zahedi MJ, Heidari M, Mohajeri M (2004) Study the effect of Valeriana Officinalis and Echium Amoenum on the liver and renal function tests in rats. J Kerman Univ Med Sci 11:22–27
Zendehdel M, Barati A, Alikhani H (2010) Synthesis and characterization of poly (AAm-co-AAc)/NaA nanocomposite and removal of methylene blue with it. J Iran Chem Res 3:161–165
Zhang L, Pornpattananangkul D, Hu C-M, Huang C-M (2010) Development of nanoparticles for antimicrobial drug delivery. Curr Med Chem 17:585–594.
https://doi.org/10.2174/092986710790416290 CrossRef PubMed Google Scholar Copyright information
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