, Volume 26, Issue 18, pp 9439–9446 | Cite as

Effects of alkaline hydrogen peroxide treatment on cellulose accessibility of switchgrass pretreated by acidic deep eutectic solvent

  • Zhu Chen
  • Yisheng Sun
  • Caixia WanEmail author
Original Research


Choline chloride: lactic acid (ChCl:LA) is a promising green acidic deep eutectic solvent for biomass fractionation and lignin extraction. This study reported an efficient ChCl:LA pretreatment for delignification and xylan solubilization from biomass. Under a mild condition (30 min, 130 °C), 83.4% lignin and 80.4% xylan were selectively removed from switchgrass. The pretreated switchgrass was highly enriched with cellulose (71.4%), but showed slow release of sugar. Its enzymatic hydrolysis efficiency was much improved by alkaline hydrogen peroxide (AHP) post-treatment. Synergistic effects of AHP suggested that additional physical barrier to cellulose accessibility could be created through side reactions between LA and cellulose. After AHP post-treatment, cellulose became highly accessible and digestible, with 94.1% glucose yield even at a high solid loading (20%). ChCl:LA can be recycled and reused without conditioning although pretreatment effectiveness diminished gradually. Overall, an efficient ChCl:LA pretreatment was developed for biomass pretreatment, and possible interaction between LA and cellulose during the pretreatment can be decoupled by AHP post-treatment.


Deep eutectic solvent Choline chloride Lactic acid Pretreatment Enzymatic hydrolysis Lignocellulosic biomass Cellulose 


Compliance with ethical standards

Conflict of interest

The authors declare that they have no competing interests.

Supplementary material

10570_2019_2759_MOESM1_ESM.pdf (459 kb)
Supplementary material 1 (PDF 460 kb)


  1. Alvarez-Vasco C, Ma R, Quintero M, Guo M, Geleynse S, Ramasamy KK, Wolcott M, Zhang X (2016) Unique low-molecular-weight lignin with high purity extracted from wood by deep eutectic solvents (DES): a source of lignin for valorization. Green Chem 18:5133–5141. CrossRefGoogle Scholar
  2. Banerjee G, Car S, Liu T, Williams DL, Meza SL, Walton JD, Hodge DB (2012) Scale-up and integration of alkaline hydrogen peroxide pretreatment, enzymatic hydrolysis, and ethanolic fermentation. Biotechnol Bioeng 109:922–931. CrossRefPubMedGoogle Scholar
  3. Caspeta L, Caro-Bermúdez MA, Ponce-Noyola T, Martinez A (2014) Enzymatic hydrolysis at high-solids loadings for the conversion of agave bagasse to fuel ethanol. Appl Energy 113:277–286. CrossRefGoogle Scholar
  4. Chen Z, Wan C (2018) Ultrafast fractionation of lignocellulosic biomass by microwave-assisted deep eutectic solvent pretreatment. Bioresour Technol 250:532–537. CrossRefPubMedGoogle Scholar
  5. Chen Z, Bai X, Lusi A, Wan C (2018) High-solid lignocellulose processing enabled by natural deep eutectic solvent for lignin extraction and industrially relevant production of renewable chemicals. ACS Sustain Chem Eng 6:12205–12216. CrossRefGoogle Scholar
  6. David K, Ragauskas AJ (2010) Switchgrass as an energy crop for biofuel production: a review of its ligno-cellulosic chemical properties. Energy Environ Sci 3:1182–1190. CrossRefGoogle Scholar
  7. French A (2014) Idealized powder diffraction patterns for cellulose polymorphs. Cellulose 21:885–896. CrossRefGoogle Scholar
  8. Ho MC, Ong VZ, Wu TY (2019) Potential use of alkaline hydrogen peroxide in lignocellulosic biomass pretreatment and valorization—a review. Renew Sustain Energy Rev 112:75–86. CrossRefGoogle Scholar
  9. Jiang W, Peng H, Li H, Xu J (2014) Effect of acetylation/deacetylation on enzymatic hydrolysis of corn stalk. Biomass Bioenergy 71:294–298. CrossRefGoogle Scholar
  10. Kim KH, Dutta T, Sun J, Simmons B, Singh S (2018) Biomass pretreatment using deep eutectic solvents from lignin derived phenols. Green Chem 20:809–815. CrossRefGoogle Scholar
  11. Kumar L, Chandra R, Saddler J (2011) Influence of steam pretreatment severity on post-treatments used to enhance the enzymatic hydrolysis of pretreated softwoods at low enzyme loadings. Biotechnol Bioeng 108(10):2300–2311. CrossRefPubMedGoogle Scholar
  12. Kumar AK, Parikh BS, Pravakar M (2016) Natural deep eutectic solvent mediated pretreatment of rice straw: bioanalytical characterization of lignin extract and enzymatic hydrolysis of pretreated biomass residue. Environ Sci Pollut Res 23:9265–9275. CrossRefGoogle Scholar
  13. Kumar AK, Shah E, Patel A, Sharma S, Dixit G (2018) Physico-chemical characterization and evaluation of neat and aqueous mixtures of choline chloride + lactic acid for lignocellulosic biomass fractionation, enzymatic hydrolysis and fermentation. J Mol Liq 271:540–549. CrossRefGoogle Scholar
  14. Liu T, Williams DL, Pattathil S, Li M, Hahn MG, Hodge DB (2014) Coupling alkaline pre-extraction with alkaline-oxidative post-treatment of corn stover to enhance enzymatic hydrolysis and fermentability. Biotechnol Biofuels 7:48. CrossRefPubMedPubMedCentralGoogle Scholar
  15. Nguyen TY, Cai CM, Osman O, Kumar R, Wyman CE (2016) CELF pretreatment of corn stover boosts ethanol titers and yields from high solids SSF with low enzyme loadings. Green Chem 18:1581–1589. CrossRefGoogle Scholar
  16. Pan X, Gilkes N, Saddler JN (2006) Effect of acetyl groups on enzymatic hydrolysis of cellulosic substrates. Holzforschung 60:398–401. CrossRefGoogle Scholar
  17. Rasmussen H, Sørensen HR, Meyer AS (2014) Formation of degradation compounds from lignocellulosic biomass in the biorefinery: sugar reaction mechanisms. Carbohydr Res 385:45–57. CrossRefPubMedGoogle Scholar
  18. Segal L, Creely J, Martin A Jr, Conrad C (1959) An empirical method for estimating the degree of crystallinity of native cellulose using the X-ray diffractometer. Text Res J 29:786–794. CrossRefGoogle Scholar
  19. Shen X-J, Wen J-L, Mei Q-Q, Chen X, Sun D, Yuan T-Q, Sun R-C (2019) Facile fractionation of lignocelluloses by biomass-derived deep eutectic solvent (DES) pretreatment for cellulose enzymatic hydrolysis and lignin valorization. Green Chem 21:275–283. CrossRefGoogle Scholar
  20. Sluiter A, Hames B, Ruiz R, Scarlata C, Sluiter J, Templeton D, Crocker D (2008) Determination of structural carbohydrates and lignin in biomass. National Renewable Energy Laboratory, Technical Report NREL/TP-510-42618Google Scholar
  21. Tang S, Liu R, Sun FF, Dong C, Wang R, Gao Z, Zhang Z, Xiao Z, Li C, Li H (2017) Bioprocessing of tea oil fruit hull with acetic acid organosolv pretreatment in combination with alkaline H2O2. Biotechnol Biofuels 10:86. CrossRefPubMedPubMedCentralGoogle Scholar
  22. Wu R, Zhao X, Liu D (2016) Structural features of formiline pretreated sugar cane bagasse and their impact on the enzymatic hydrolysis of cellulose. ACS Sustain Chem Eng 4:1255–1261. CrossRefGoogle Scholar
  23. Yang B, Boussaid A, Mansfield SD, Gregg DJ, Saddler JN (2002) Fast and efficient alkaline peroxide treatment to enhance the enzymatic digestibility of steam−exploded softwood substrates. Biotechnol Bioeng 77:678–684. CrossRefPubMedGoogle Scholar
  24. Zhang C-W, Xia S-Q, Ma P-S (2016) Facile pretreatment of lignocellulosic biomass using deep eutectic solvents. Bioresour Technol 219:1–5. CrossRefGoogle Scholar

Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  1. 1.Department of Biomedical, Bioengineering, and Chemical EngineeringUniversity of MissouriColumbiaUSA

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