, Volume 26, Issue 18, pp 9423–9438 | Cite as

Polysaccharide extraction from sugarcane leaves: combined effects of different cellulolytic pretreatment and extraction methods

  • Pei Ling Tang
  • Erwei Hao
  • Zhengcai Du
  • Jiagang Deng
  • Xiaotao HouEmail author
  • Jianfeng Qin
Original Research


This research was conducted to determine the combined effects of different cellulolytic pretreatments (cellulase vs. mixed enzymes) and extraction methods (water vs. deep eutectic solvent, DES) on the yield, chemical and functional properties of polysaccharide (PS) from sugarcane leaves (SCLs). The DESs used were choline chloride-1,4-butanediol (DESB) and choline chloride-urea (DESU). The SCLs were initially enzyme-pretreated, followed by extraction using water and DES respectively. The produced crude polysaccharide extracts (CPSs) were characterized via FTIR, total phenolics, DPPH free radical scavenging activity and in vitro simulated gastrointestinal analysis. The results indicated that cellulolytic pretreatment improved the PS yield by 14–16%, but reduced the solubility, DPPH activity and gastrointestinal digestibility of CPS. DES-CPSs possessed higher solubility and DPPH activity than water-CPSs. FTIR analysis unveiled that lignin–carbohydrate-complex was likely the component that restricted the solubility and probably the digestibility of water-CPSs. This study concluded that pretreatment and extraction procedures distinctively affected the chemical characteristics, and subsequently the functional properties of CPS.

Graphic abstract


Sugarcane leaves Polysaccharide Cellulolytic pretreatment Antioxidant activity Extraction method Gastrointestinal digestibility 



The authors would like to thank China-ASEAN Technology Transfer Center, Guangxi Science and Technology Department for the financial support in this research under the China-ASEAN Talented Young Scientist Guangxi Program.

Compliance with ethical standards

Conflict of interest

We declared there is no conflict of interest in this study.


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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  1. 1.Guangxi Key Laboratory of Efficacy Study on Chinese Materia MedicaGuangxi University of Chinese MedicineNanningChina
  2. 2.Department of Bioscience, Faculty of Applied SciencesTunku Abdul Rahman University CollegeSetapak, W. P. Kuala LumpurMalaysia
  3. 3.Collaborative Innovation Center for Research on Functional Ingredients of Agricultural ResiduesGuangxi University of Chinese MedicineNanningChina

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