Advertisement

Cellulose

, Volume 26, Issue 18, pp 9423–9438 | Cite as

Polysaccharide extraction from sugarcane leaves: combined effects of different cellulolytic pretreatment and extraction methods

  • Pei Ling Tang
  • Erwei Hao
  • Zhengcai Du
  • Jiagang Deng
  • Xiaotao HouEmail author
  • Jianfeng Qin
Original Research
  • 100 Downloads

Abstract

This research was conducted to determine the combined effects of different cellulolytic pretreatments (cellulase vs. mixed enzymes) and extraction methods (water vs. deep eutectic solvent, DES) on the yield, chemical and functional properties of polysaccharide (PS) from sugarcane leaves (SCLs). The DESs used were choline chloride-1,4-butanediol (DESB) and choline chloride-urea (DESU). The SCLs were initially enzyme-pretreated, followed by extraction using water and DES respectively. The produced crude polysaccharide extracts (CPSs) were characterized via FTIR, total phenolics, DPPH free radical scavenging activity and in vitro simulated gastrointestinal analysis. The results indicated that cellulolytic pretreatment improved the PS yield by 14–16%, but reduced the solubility, DPPH activity and gastrointestinal digestibility of CPS. DES-CPSs possessed higher solubility and DPPH activity than water-CPSs. FTIR analysis unveiled that lignin–carbohydrate-complex was likely the component that restricted the solubility and probably the digestibility of water-CPSs. This study concluded that pretreatment and extraction procedures distinctively affected the chemical characteristics, and subsequently the functional properties of CPS.

Graphic abstract

Keywords

Sugarcane leaves Polysaccharide Cellulolytic pretreatment Antioxidant activity Extraction method Gastrointestinal digestibility 

Notes

Acknowledgments

The authors would like to thank China-ASEAN Technology Transfer Center, Guangxi Science and Technology Department for the financial support in this research under the China-ASEAN Talented Young Scientist Guangxi Program.

Compliance with ethical standards

Conflict of interest

We declared there is no conflict of interest in this study.

References

  1. Brienzo M, Fikizolo S, Benjamin Y, Tyhoda L, Gorgens J (2017) Influence of pretreatment severity on structural changes, lignin content and enzymatic hydrolysis of sugarcane bagasse samples. Renew Energy 104:271–280CrossRefGoogle Scholar
  2. Carvalho DM, Martinez-Abad A, Evtuguin DV, Colodette JL, Lindstrom ME, Vilaplana F, Sevastyanova O (2017) Isolation and characterization of acetylated glucuronoarabinoxylan from sugarcane bagasse and straw. Carbohydr Polym 156:223–234CrossRefGoogle Scholar
  3. Chen C, Huang Q, You L, Fu X (2017) Chemical property and impacts of different polysaccharide fraction from Fructus Mori on lipolysis with digestion model in vitro. Carbohydr Polym 178:360–367CrossRefGoogle Scholar
  4. Conag AT, Villahermosa JER, Cabatingan LK, Go AW (2018) Energy densification of sugarcane leaves through torrefaction under minimized oxidative atmosphere. Energy Sustain Dev 42:160–169CrossRefGoogle Scholar
  5. Das AK, Sharma M, Mondal D, Prasad K (2015) Deep eutectic solvent as efficient solvent system for the extraction of κ-carrageenan from Kappaphycus alvarezii. Carbohydr Polym 136:903–935Google Scholar
  6. Gao H, Yu Z, He Q, Tang S, Zeng W (2018) A potentially functional yogurt co-fermentation with Gnaphalium affine. LWT Food Sci Technol 91:423–430CrossRefGoogle Scholar
  7. Getachew AT, Cho YJ, Chun BS (2018) Effect of pretreatments on isolation of bioactive polysaccharides from spent coffee grounds using subcritical water. Int J Biol Macromol 109:711–719CrossRefGoogle Scholar
  8. Gusakov AV, Kondratyeva EG, Sinitsyn AP (2011) Comparison of two methods for assaying reducing sugars in the determination of carbohydrase activities. Int J Anal Chem.  https://doi.org/10.1155/2011/283658 CrossRefPubMedPubMedCentralGoogle Scholar
  9. Hamlaoui I, Bencheraiet R, Bensegueni R, Bencharif M (2018) Experimental and theoretical study on DPPH radical scavenging mechanism of some chalcone quinoline derivatives. J Mol Struct 1156:385–389CrossRefGoogle Scholar
  10. Hao E, He Y, Hou X, Du Z, Deng J (2018) Preventive effects and the mechanism of the polysaccharide from sugarcane leaves on non-obese diabetic mice. West China J Pharma 33:481–484Google Scholar
  11. He T, Hu S, Hou X, Qin C (2016) The effects of sugarcane leaves polysaccharide on the ECG and angiogenesis in rats with myocardial infarction. J Guangxi Med Univ 33:229–231Google Scholar
  12. He L, Yan X, Liang J, Li S, He H, Xiong Q, Lai X, Hou S, Huang S (2018) Comparison of different extraction methods for polysaccharides from Dendrobium officinale stem. Carbohydr Polym 198:101–108CrossRefGoogle Scholar
  13. Herrera-Marquez O, Fernandez-Serrano M, Pilamala M, Jacome MB, Luzon G (2019) Stability studies of an amylase and a protease for cleaning processes in food industry. Food Bioprod Process 117:64–73CrossRefGoogle Scholar
  14. Hu Z, Wang P, Zhou H, Li Y (2018) Extraction, characterization and in vitro antioxidant activity of polysaccharides from Carex meyeriana Kunth using different methods. Int J Biol Macromol 120:2155–2164CrossRefGoogle Scholar
  15. Jutakanoke R, Leepipatpiboon N, Tolieng V, Kitpreechavanich V, Srinorakutara T, Akaracharanya A (2012) Sugarcane leaves: pretreatment and ethanol fermentation by Saccharomyces cerevisiae. Biomass Bioenergy 39:283–289CrossRefGoogle Scholar
  16. Kumar KP, Reddy VR, Prakash MG, Kumar KP (2018) In vitro estimation of total phenolics and DPPH radicals scavenging activity of Withania somnifera extract. Pharma Innov J 7:588–590Google Scholar
  17. Lee C, Chen Z, Yu P, Yen W, Lin K, Duh P (2013) Comparison of protective effects of three varieties of sugarcane leaves on oxidative stress in Clone 9 cells. J Funct Food 5:878–887CrossRefGoogle Scholar
  18. Li Z, Ge Y (2012) Antioxidant activities of lignin extracted from sugarcane bagasse via different chemical procedures. Int J Biol Macromol 51:1116–1120CrossRefGoogle Scholar
  19. Lin K, Liu D, He T, Hou X (2018) The effect and mechanism of sugarcane leaves polysaccharide on dynamic electrocardiogram and cardiac function in rats with myocardial infarction. J Guangxi Med Univ 35:640–643Google Scholar
  20. Malaeke H, Housaindokht MR, Monhemi H, Izadyar M (2018) Deep eutectic solvent as an efficient molecular liquid for lignin solubilization and wood delignification. J Mol Liq 263:193–199CrossRefGoogle Scholar
  21. Martins MTB, Souza WRD, Cunha BADBD, Basso MF, Oliveira NGD, Vinecky F, Martins PK, Oliveira PAD, Arenque-Musa BC, Souza APD, Buckeridge MS, Kobayashi AK, Quirino BF, Molinari HBC (2016) Characterization of sugarcane (Saccharum spp.) leaf senescence: Implications for biofuel production. Biotechnol Biofuels 9:153CrossRefGoogle Scholar
  22. Masuko T, Minami A, Iwasaki N, Majima T, Nishimura S, Lee YC (2005) Carbohydrate analysis by a phenol-sulfuric acid method in microplate format. Anal Biochem 339:69–72CrossRefGoogle Scholar
  23. Patel AK, Singhania RR, Sim SJ, Pandey A (2019) Thermostable cellulases: current status and perspectives. Bioresour Technol 279:385–392CrossRefGoogle Scholar
  24. Peng Y, Liu R, Cao J (2015) Characterization of surface chemistry and crystallization behavior of polypropylene composites reinforced with wood flour, cellulose and lignin during accelerated weathering. Appl Surf Sci 332:252–259CrossRefGoogle Scholar
  25. Rein MJ, Renouf M, Cruz-Hernandez C, Actis-Goretta L, Thakkar SK, Pinto MS (2013) Bioavailability of bioactive food compounds: a challenging journey to bioefficacy. Br J Clin Pharmacol 75:588–602CrossRefGoogle Scholar
  26. Rozi P, Abuduwaili A, Mutailifu P, Gao Y, Rakhmanberdieva R, Aisa HA, Yili A (2019) Sequential extraction, characterization and antioxidant activity of polysaccharides from Fritillaria pallidiflora Schrenk. Int J Biol Macromol 131:97–106CrossRefGoogle Scholar
  27. Santos ABD, Bottcher A, Vicentini R, Mayer JLS, Kiyota E, Landell MAG, Creste S (2015) Lignin biosynthesis in sugarcane is affected by low temperature. Environ Exp Bot 120:31–42CrossRefGoogle Scholar
  28. Sharazi AM, Heiningen ARP, Sumerskii I, Bacher M (2018) Sugarcane straw lignin obtained by sulfur-dioxide-alcohol-water (SAW) fractionation: effect of solvent. Ind Crop Prod 115:235–242CrossRefGoogle Scholar
  29. Sindhu R, Gnansounou E, Binod P, Pandey A (2016) Bioconversion of sugarcane crop residue for value added products—an overview. Renew Energy 98:203–215CrossRefGoogle Scholar
  30. Sluiter A, Hames B, Hyman D, Payne C, Ruiz R, Scarlata C, Sluiter J, Templeton D, Wolfe J (2008a) Determination of total solids in biomass and total dissolved solids in liquid process samples. Technical report NREL/TP-510-42621. National Renewable Energy Laboratory, ColoradoGoogle Scholar
  31. Sluiter A, Hames B, Ruiz R, Scarlata C, Sluiter J, Templeton D (2008b) Determination of ash in biomass. Technical report NREL/TP-510-42622. National Renewable Energy Laboratory, ColoradoGoogle Scholar
  32. Sluiter A, Ruiz R, Scarlata C, Sluiter J, Templeton D (2008) Determination of extractives in biomass. Technical report NREL/TP-510-42619. National Renewable Energy Laboratory, ColoradoGoogle Scholar
  33. Sluiter A, Hames B, Ruiz R, Scarlata C, Sluiter J, Templeton D, Crocker D (2012) Determination of structural carbohydrates and lignin in biomass. Technical report NREL/TP-510-42618. National Renewable Energy Laboratory, ColoradoGoogle Scholar
  34. Souza APD, Leite DCC, Pattathil S, Hahn MG, Buckeridge MS (2013) Composition and structure of sugarcane cell wall polysaccharides: implications for second generation bioethanol production. Bioenergy Res 6:564–579CrossRefGoogle Scholar
  35. Sunthornvarabhas J, Liengprayoon S, Suwonsichon T (2017) Antimicrobial kinetic activities of lignin from sugarcane bagasse for textile product. Ind Crop Prod 109:857–861CrossRefGoogle Scholar
  36. Tadayoni M, Sheikh-Zeinoddin M, Soleimanian-Zad S (2015) Isolation of bioactive polysaccharide from acorn and evaluation of its functional properties. Int J Biol Macromol 72:179–184CrossRefGoogle Scholar
  37. Wang F, Yang G, Ke W, Ma S (2018a) Studies on suspension property of sugarcane components. IFAC Pap OnLine 51–17:526–531CrossRefGoogle Scholar
  38. Wang Y, Li Y, Ma X, Ren H, Fan W, Leng F, Yang M, Wang X (2018b) Extraction, purification and bioactivities analyses of polysaccharides from Glycyrrhiza uralensis. Ind Crop Prod 122:596–608CrossRefGoogle Scholar
  39. Wu Y, Wei Z, Zhang F, Linhardt RJ, Sun P, Zhang A (2019) Structure, bioactivities and applications of the polysaccharides from Tremella fuciformis mushroom: a review. Int J Biol Macromol 121:1005–1010CrossRefGoogle Scholar
  40. Yuan Y, Li C, Zheng Q, Wu J, Zhu K, Shen X, Cao J (2019) Effect of simulated gastrointestinal digestion in vitro on the antioxidant activity, molecular weight and microstructure of polysaccharides from a tropical sea cucumber (Holothuria leucospilota). Food Hydrocoll 89:735–741CrossRefGoogle Scholar
  41. Zhang L, Wang M (2017) Optimization of deep eutectic solvent-based ultrasound-assisted extraction of polysaccharides from Dioscorea opposita Thunb. Int J Biol Macromol 95:675–681CrossRefGoogle Scholar
  42. Zhao Z, Yan H, Zheng R, Saeed KM, Fu X, Tao Z, Zhang Z (2018) Anthocyanins characterization and antioxidant activities of sugarcane (Saccharum officinarum L.) rind extracts. Ind Crop Prod 113:38–45CrossRefGoogle Scholar
  43. Zheng R, Su S, Li J, Zhao Z, Wei J, Fu X, Liu RH (2017) Recovery of phenolics from the ethanolic extract of sugarcane (Saccharum officinarum L.) bagasse and evaluation of the antioxidant and antiproliferative activities. Ind Crop Prod 107:360–369CrossRefGoogle Scholar
  44. Zietsman AJJ, Moore JP, Fangel JU, Willats WGT, Vivier MA (2017) Combining hydrothermal pretreatment with enzymes de-pectinates and exposes the innermost xyloglucan-rich hemicellulose layers of wine grape pomace. Food Chem 232:340–350CrossRefGoogle Scholar

Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  1. 1.Guangxi Key Laboratory of Efficacy Study on Chinese Materia MedicaGuangxi University of Chinese MedicineNanningChina
  2. 2.Department of Bioscience, Faculty of Applied SciencesTunku Abdul Rahman University CollegeSetapak, W. P. Kuala LumpurMalaysia
  3. 3.Collaborative Innovation Center for Research on Functional Ingredients of Agricultural ResiduesGuangxi University of Chinese MedicineNanningChina

Personalised recommendations