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Cellulose

pp 1–13 | Cite as

Synthesis of a polydopamaine nanoparticle/bacterial cellulose composite for use as a biocompatible matrix for laccase immobilization

  • Rui Zhai
  • Xiangxue Chen
  • Mingjie JinEmail author
  • Jinguang HuEmail author
Original Research

Abstract

Bacterial cellulose has attracted attention as a scaffolding material for enzyme immobilization because of its excellent mechanical properties, nanoporous structure, high purity, and super hydrophilicity. However, the lack of reactive group and the relatively low binding capacity toward biomolecules limit its application. In this study, we have synthesized a polydopamine nanoparticle/bacterial cellulose hybrid material with hierarchical multiscale architecture for enzyme immobilization, via a simple in situ self-assembly of polydopamine nanoparticles on cellulose surface. Commercial enzyme laccases were employed to evaluate the enzyme immobilizability of this hybrid material, and various key parameters of enzyme immobilization such as reaction time, enzyme concentration, temperature and pH were also systematically studied. This hybrid composite displayed excellent enzyme immobilization efficiency (~ 165 mg laccase per g composite), and the immobilized laccase also exhibited better thermostability and operational stability over a broad temperature range as compared with free laccase. By using the immobilized laccase, fast and efficient dye removal ability and good recyclability were achieved during the dye decolorization test, which demonstrated its potential application in various industrial relevant wastewater treatment processes.

Graphic abstract

Keywords

Bacterial cellulose Polydopamine nanoparticles Laccase Enzyme immobilization Wastewater treatment 

Notes

Acknowledgments

This work was financially supported by National Key R&D Program of China (Grant No. 2016YFE0105400), Natural Science and Engineering Research Council (NSERC) Canada First Research Excellence Fund, National Natural Science Foundation of China (Grant No. 21606132), Natural Science Foundation of Jiangsu Province (Grant Nos. BK20160823, BK20170037 and BK20170832), the Fundamental Research Funds for the Central Universities (Grant No. 30916011202), the Foundation of Jiangsu Specially-Appointed Professor and the Foundation of Jiangsu Innovative and Entrepreneurial Doctors. The authors thank Prof. Qiang Zhang from Nanjing University of Science and Technology for his assistance in FT-IR measurement.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  1. 1.School of Environmental and Biological EngineeringNanjing University of Science and TechnologyNanjingChina
  2. 2.Department of Chemical and Petroleum EngineeringUniversity of CalgaryCalgaryCanada

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